50results about How to "Simplifies isolation" patented technology

Disclosed are immortalized human embryonic retina cells, having a nucleic acid sequence encoding an adenoviral E1A protein integrated into the genome of the cells, and further comprising a nucleic acid sequence encoding an enzyme involved in post-translational modification of proteins, such as a sialyltransferase, wherein said nucleic acid sequence encoding the enzyme involved in post-translational modification of proteins is under control of a heterologous promoter. Methods for producing recombinant proteins from such cells and obtaining such recombinant proteins having increased sialylation are provided as are novel compositions of isoforms of erythropoietin .

Dehydrohalogenation processes for the preparation of fluoropropenes from corresponding halopropanes, in which the fluoropropenes have the formula CF3CY═CXNHP, wherein X and Y are independently hydrogen or a halogen selected from fluorine, chlorine, bromine and iodine; and N and P are independently integers equal to 0, 1 or 2, provided that (N+P)=2.

Dehydrohalogenation processes for the preparation of fluoropropenes from corresponding halopropanes, in which the fluoropropenes have the formula CF3CY═CXNHP, wherein X and Y are independently hydrogen or a halogen selected from fluorine, chlorine, bromine and iodine; and N and P are independently integers equal to 0, 1 or 2, provided that (N+P)=2.

To conduct a fluid, the transducer has a flow tube which in operation vibrated by an excitation assembly. Inlet-side and outlet-side vibrations of the flow tube are sensed by means of a sensor arrangement. To produce shear forces in the fluid, the flow tube is at least intermittently excited into torsional vibrations about a longitudinal flow-tube axis. An internal portion of the transducer, formed at least by the flow tube, an antivibrator, the sensor arrangement, and the excitation assembly and mounted at least on the inlet and outlet tube sections, has a centroid which is located inside the flow tube. The transducer is suitable for use in viscometers or Coriolis mass flowmeter-viscometers. In spite of using only a single straight flow tube, it is dynamically well balanced in operation, and the development of bending moments by the torsionally vibrating flow tube is largely prevented. This also effectively prevents the transducer case or the connected pipe from being excited into sympathetic vibration. Measurement signals representative of mass flow rate are readily distinguishable from measurement signals representative of viscosity, particularly if the sensors used for the viscosity measurement are also used for the mass flow measurement.

The invention describes a process for preparing acetone starting from acetyl-coenzyme A comprising process steps A. enzymatic conversion of acetyl-CoA into acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA and C. decarboxylation of acetoacetate to acetone and CO2, which is characterized in that the coenzyme A is not transferred in process step B to an acceptor molecule. In addition, process step B is surprisingly catalysed by enzymes of the classes of acyl-CoA thioesterase, acyl-CoA synthetase or acyl-CoA thiokinase.A completely novel metabolic pathway is concerned, because the enzymatic hydrolysis of acetoacetyl-CoA without simultaneous transfer of CoA to a receptor molecule has never previously been described for any microbial enzyme.

Disclosed are immortalized human embryonic retina cells, having a nucleic acid sequence encoding an adenoviral E1A protein integrated into the genome of the cells, and further comprising a nucleic acid sequence encoding an enzyme involved in post-translational modification of proteins, such as a sialyltransferase, wherein said nucleic acid sequence encoding the enzyme involved in post-translational modification of proteins is under control of a heterologous promoter. Methods for producing recombinant proteins from such cells and obtaining such recombinant proteins having increased sialylation are provided as are novel compositions of isoforms of erythropoietin.

A commercial scale continuous process for the production of a bisphenol comprising reacting a phenol and a ketone in the presence of a modified ion exchange resin catalyst, wherein said modified ion exchange resin comprises a crosslinked gellular acid functionalized polystyrene resin modified by neutralization of with a mercapto promoter.

The present application is directed to stabilized HIV envelope glycoprotein trimers. The trimers are stabilized by introducing trimeric motifs, preferably the GCN4 coiled coil or the fibritin trimeric domain, at certain sites, for example in the gp41 ectodomain. These stabilized trimers or DNA molecules encoding such trimers can be used to generate an immunogenic reaction. The trimers can also be used in assays to screen for molecules that interact with them—and to identify molecules that interact with specific sites.

In a semiconductor laser module with an optical isolator where the form of a metal holder for holding a fiber stub varies, optical-isolator parts are miniaturized and unified, simplifying product management, reducing delivery time and cost. A fiber stub with an optical isolator is provided in such a manner that a stepped surface is formed on an end surface of a ferrule, where an optical fiber has been inserted and secured to a through hole formed at the center of the ferrule. The fiber stub is formed by attaching an optical isolator element composed of a polarizer and a Faraday rotator to the stepped surface.

Process for the preparation of 2,3,5-trimethyl-p-benzoquinone by oxidizing 2,3,5-trimethylphenol or 2,3,6-trimethylphenol with oxygen or an oxygen-containing gas mixture in the presence of a catalyst system which can be a copper halide and a transition metal halide; for example iron, chromium, manganese, cobalt, nickel, zinc or a rare earth halide, in a two-phase reaction medium, at elevated temperature.

The present invention relates to novel 1,3-diimine copper complexes and the use of 1,3-diimine copper complexes for the deposition of copper on substrates or in or on porous solids in an Atomic Layer Deposition process. This invention also provides a process for making amino-imines and novel amino-imines.

A test circuit is provided, which is independent of a power supply and which enables a circuit breaker to be tested in a reliable manner. The test circuit includes a test coil which is devoid of electric potential and is wound around a totalizing current transformer. The test coil is preferably short circuited using a test switch and a connectable load. This simulates the occurrence of a residual current. The selection of an appropriate connectable load allows the sensitivity of the current circuit breaker to be tested in an advantageous manner.

In a semiconductor laser module with an optical isolator where the form of a metal holder for holding a fiber stub varies, optical-isolator parts are miniaturized and unified, simplifying product management, reducing delivery time and cost. A fiber stub with an optical isolator is provided in such a manner that a stepped surface is formed on an end surface of a ferrule, where an optical fiber has been inserted and secured to a through hole formed at the center of the ferrule. The fiber stub is formed by attaching an optical isolator element composed of a polarizer and a Faraday rotator to the stepped surface.

Disclosed are compounds which inhibit beta-amyloid peptide release and / or its synthesis, and, accordingly, have utility in treating Alzheimer's disease. Also disclosed pharmaceutical compositions comprising a compound which inhibits beta-amyloid peptide release and / or its synthesis as well as methods for treating Alzheimer's disease both prophylactically and therapeutically with such pharmaceutical compositions.

An aircraft includes a supply system with an electrochemical reactor for generating energy, hydrogen, oxygen and clear water from grey water, a hydrocarbon containing fuel and air, so that the up to now separated systems for supply of clear water, generation of energy and generation of oxygen are combined to a combined system. The necessary redundancies may thereby secured by two or more systems of the same kind, which may be operated completely independently of each other.

The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.

Olopatadine can be obtained by means of a process comprising hydrolysis of a compound of general formula (II), wherein Y is OR1, wherein R1 is C1-C7 alkyl, C3-C7 cycloalkyl, aryl, arylalkyl, or heterocycle; or NR2R3, wherein R2 and R3, independently from each other, are C1-C7 alkyl, aryl, arylalkyl, or R2 and R3 together with the nitrogen atom to which they are bound form a heterocycle of 3 to 7 members, obtained by means of a process comprising reacting the corresponding ester or amide of 6,11-dihydro-11-oxodibenz[b,e]oxepin-2-acetic acid with a suitable Wittig reagent, in the presence of a base in a reaction medium comprising an organic solvent.

An aircraft includes a supply system with an electrochemical reactor for generating energy, hydrogen, oxygen and clear water from grey water, a hydrocarbon containing fuel and air, so that the up to now separated systems for supply of clear water, generation of energy and generation of oxygen are combined to a combined system. The necessary redundancies may thereby secured by two or more systems of the same kind, which may be operated completely independently of each other.

Cytotoxic αβ T cells form an essential component in immunity to infections and tumors, and are also implicated in host defense against these challenges. The present disclosure demonstrates the ability of activated γδ T cells to cross-present exogenous antigens to CD8+αβ T cells, a process previously thought to be mediated best by dendritic cells. In particular, the present disclosure provides a method for cross-presentation of antigen derived from tumor cell or microbial organisms such as viruses, bacteria, yeasts, parasites, and the like, or from cells infected with such organisms, to a CD8+αβ T cell. Still further, the present disclosure provides a method for treatment of a tumor or a chronic or recurrent infectious disease, comprising delivering an antigen-presenting autologous γδ T cell population above into a patient requiring such treatment. Still yet further, a method is described for preparing a peptide-specific effector T cell.

One aspect of the present invention relates to a reporter system for detection of protein-protein interactions in the periplasm of a prokaryotic host cell. The reporter system includes a first expression system which has a nucleic acid molecule encoding a first fragment of a reporter protein molecule, a nucleic acid molecule encoding a first signal sequence, and a nucleic acid molecule encoding a first member of a putative binding pair, where the nucleic acid molecule encoding the first fragment, the nucleic acid molecule encoding the first signal sequence, and the nucleic acid molecule encoding the first member of the putative binding pair are operatively coupled to permit their expression in a prokaryotic host cell as a first fusion protein. The reporter sys tem also includes a second expression system which has a nucleic acid molecule encoding a second fragment of the reporter protein molecule, a nucleic acid molecule encoding a second signal sequence, and a nucleic acid molecule encoding a second member of the putative binding pair, where the nucleic acid molecule encoding the second fragment, the nucleic acid molecule encoding the second signal sequence, and the nucleic acid molecule encoding the second member of the putative binding pair are operatively coupled to permit their expression in a prokaryotic host cell as a second fusion protein, where, when expressed in a prokaryotic host cell, at least one of the first and the second fusion proteins are co-translationally transported to the periplasm where, when present, the first and second members of the putative binding pair bind together and the first and second fragments of the reporter protein molecule are reconstituted, thereby producing an active reporter protein. The reporter system may be used to carry out methods of identifying candidate compounds which bind to a target protein, identifying a candidate gene which modulates binding between a first protein and second protein, and identifying a candidate compound which modulates binding between a first protein and second protein.

Process for the production of unsaturated ethers, in particular isopropenyl methyl ether (IPM), by pyrolysis of a ketal-containing or acetal-containing mixture, in particular dimethoxypropane (DMP), in the liquid phase in the presence of an organic carboxylic acid, according to the following reaction scheme: wherein R1=H or alkyl with 1-8 C atoms; R2=H, CH3-, C2H5-, or Cl-; R3=alkyl with 1-8 C atoms; R4=H, CH3-, C2H5-, or C3H7-, and R1 and R4 may be joined to form a 5-, 6-, or 7-membered ring. DMP is produced by the process from acetone and methanol by reaction in an acidic heterogeneous ion exchanger, the product being isolated by extraction with aqueous alkaline solution. In particular the process involves combining the IPM reaction product with the DMP reaction product, which permits the execution of a stable recycling process in which the methanol-containing streams of the IPM and DMP stages can be simultaneously extracted. The product may be isolated by the process by simple distillation of IPM from a mixture containing IPM, DMP and acetone.

To conduct a fluid, the transducer has a flow tube which in operation is vibrated by an excitation assembly and whose inlet-side and outlet-side vibrations are sensed by means of a sensor arrangement. To produce shear forces in the fluid, the flow tube is at least intermittently excited into torsional vibrations about a longitudinal flow-tube axis. The transducer further comprises a torsional vibration absorber which is fixed to the flow tube and which in operation covibrates with the torsionally vibrating flow tube, thus producing reactive torques which at least partially balance torques developed in the vibrating flow tube. One of the advantages of the transducer disclosed is that it is dynamically balanced to a large extent even in the face of variations in fluid density or viscosity.

The invention describes a process for preparing acetone starting from acetyl-coenzyme A comprising process steps A. enzymatic conversion of acetyl-CoA into acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA and C. decarboxylation of acetoacetate to acetone and CO2, which is characterized in that the coenzyme A is not transferred in process step B to an acceptor molecule. In addition, process step B is surprisingly catalyzed by enzymes of the classes of acyl-CoA thioesterase, acyl-CoA synthetase or acyl-CoA thiokinase.A completely novel metabolic pathway is concerned, because the enzymatic hydrolysis of acetoacetyl-CoA without simultaneous transfer of CoA to a receptor molecule has never previously been described for any microbial enzyme.

The invention relates to novel polymorphic and hydrate forms and salts of 6-{difluoro[6-(1-methyl-1H-pyrazol-4-yl)[1,2,4]triazolo[4,3-b]pyridazin-3-yl]methyl}quinoline, to methods for their preparation, to pharmaceutical compositions comprising at least one of said polymorphic or hydrate forms or salts, and to the therapeutic and / or prophylactic use of such compositions. The invention also provides new manners for preparing said compound.

Process for the preparation of alpha, beta unsaturated aldehydes by oxidation of alcohols where the oxidant is oxygen in the presence of a catalyst comprising platinum on a support in the presence of a liquid phase which contains at least 25 weight-% water based on the total weight of the liquid phase.

Disclosed is a method for producing L-citrulline using recombinant Corynebacterium crenatum cells as whole-cell biocatalysts. The present invention provides a recombinant C. crenatum that expresses an exogenous arginine deiminase gene from Lactobacillus brevis. The recombinant C. crenatum SDNN403 is used as biocatalysts for converting L-arginine to produce L-citrulline. Using the method of the invention, the concentration of L-citrulline reached 301.4 g / L after a 48 hr conversion reaction, and the molar conversion rate reached 99.9%.