Aspergillus clavatus-32 strain capable of highly yielding Lovastatin and application of Aspergillus clavatus-32 strain

A technology of ac-32 and lovastatin, which is applied in the field of biotechnology microorganisms, can solve the problems of lack of excellent industrial strains of Lovastatin, and achieve the effects of large spore production, fast growth speed and simple cultivation

Active Publication Date: 2016-01-06
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In response to the increasing demand for Lovastatin in the market, the lack of excellent industrial strains for producing Lovastatin, etc.

Method used

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  • Aspergillus clavatus-32 strain capable of highly yielding Lovastatin and application of Aspergillus clavatus-32 strain
  • Aspergillus clavatus-32 strain capable of highly yielding Lovastatin and application of Aspergillus clavatus-32 strain
  • Aspergillus clavatus-32 strain capable of highly yielding Lovastatin and application of Aspergillus clavatus-32 strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Aspergillus clavatus ( Aspergillusclavatus ) Isolation, screening and identification of Ac-32 strain

[0028] 1 medium

[0029] ① PDA medium: potato 20%, glucose 2%, agar powder 2.0%, 1L water, pH 5.5~6.0. Used for the separation, purification and identification of Aspergillus strains.

[0030] ②PD medium: potato 20%, glucose 2%, beef extract 0.5%, water 1L, pH 5.5~6.0. Used for the screening of high-yielding Aspergillus strains of Lovastatin.

[0031] 2 Experimental methods

[0032] 2.1 Separation and purification of Aspergillus strains

[0033] Pick a small amount of hyphae from the surface of natural fermentation samples (food, soil, organic matter, etc.) collected from different habitats and connect them to the surface of the PDA medium plate, and incubate at 28°C for 24 hours. After the white fluffy hyphae grow out, take a few hyphae to transfer After being connected to another PDA medium plate and continuing to culture for 3 days to produce spores, the micro...

Embodiment 2

[0046] Example 2 Aspergillus clavatus ( Aspergillusclavatus ) The growth curve of Ac-32 strain in a 5L fermentor and the law of Lovastatin production

[0047] 1 strain: Ac-32 Aspergillus strain.

[0048] 2 medium

[0049] ① PDA culture medium: the formula is the same as in Example 1.

[0050] ②PD medium: the formula is the same as in Example 1.

[0051] ③Seed culture medium: lactose 10%, glycerol 1%, peptone 0.5%, soybean powder 1%, KH 2 PO 4 0.1%, MgSO 4 7H 2 O0.1%, NaNO 3 0.2%, 1L water, pH 5.5.

[0052] ④ Fermentation medium: lactose 20%, glycerol 2%, soy peptone 1%, yeast extract 0.5%, MgSO 4 7H 2 O0.1%, NaNO 3 0.2%, KH 2 PO 4 0.1%, ZnSO 4 0.2%, 1L water, pH 5.2.

[0053] 3 experimental methods

[0054] 3.1 Cultivation of Ac-32 strain

[0055] Pick a small amount of Ac-32 strain hyphae with an inoculating needle, transfer to a PDA medium plate, and activate and culture for 3 days; take 1 bacterial cake (0.8 mm in diameter) from the plate and inoculate it into PD medium, and culture...

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Abstract

The invention belongs to the field of biotechnology microorganisms, and particularly relates to an Aspergillus clavatus-32 strain producing Lovastatin through liquid fermentation, application and a fermentation producing method. The Aspergillus clavatus-32 strain is high in Lovastatin yield and growing speed, large in sporulation quantity and simple to culture. Under the conditions that initial pH of a fermentation culture medium is 5.2 and culture temperature is 28 DEG C, after the liquid fermentation culture medium and fermentation conditions are optimized and the Ac-32 strain is fermented in a 5L fermentation tank for 110-140h, yield of Lovastatin can reach about 200-250mg/L.

Description

technical field [0001] The invention belongs to the field of biotechnology microorganisms, in particular to a strain of Aspergillus clavus that produces Lovastatin by liquid fermentation ( Aspergillus clavatus ) Ac-32 strain, application and fermentation production method. Background technique [0002] In recent years, the prevalence, incidence and risk factors of cardiovascular disease in the Chinese population have continued to rise. According to the latest data from the National Disease Surveillance System, the proportion of cardiovascular disease in total deaths in my country has increased from 37.5% to 40.7%. Lovastatin, a secondary metabolite of Aspergillus, has become one of the best-selling drugs for the treatment of cardiovascular diseases due to its clear mechanism of action and remarkable clinical efficacy in the prevention and treatment of hyperlipidemia, atherosclerosis and cardiovascular and cerebrovascular diseases . [0003] In 1979, Japanese scholar Akira ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/06C12R1/66
Inventor 蒋冬花章婷任浩戴梦怡郑逸舒
Owner ZHEJIANG NORMAL UNIVERSITY
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