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High-yield production method of lovastatin

A technology of lovastatin and a production method, applied in the production method and the field of Monascus used in the production method, can solve the problems of staying, not forming an industrialized scale, etc., and achieve the effects of high production capacity and improved quality

Active Publication Date: 2015-04-29
山东中惠生物科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for better yields from certain types of yeasts that produce high levels of esters called lithocholic acid (lactone). It also improves efficiency by allowing these enzymes to be produced at an increased rate compared to other methods such as monacolin or erythroquinoline synthesis.

Problems solved by technology

This patents discusses various methods used during commercial manufacturing of Lovitzinum (lovastatin). One problem addressed in this patented study relates to extracting highly purified forms from natural sources like Aceriaceae plants without causing harmful environmental impacts. Another issue involves producing monacerucleotide compounds called lycopene, another type of molecule found in many types of vegetables including tomatoes.

Method used

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  • High-yield production method of lovastatin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Mutation and preliminary screening of Monascus strain with high lovastatin production

[0043] Wash the spores from the growth slant of the initial strain Monascus purpureus with normal saline to obtain a spore suspension, inoculate the seed medium, and cultivate for about 36 hours. Take the bacterial suspension, filter it with sterile gauze, and shake it with glass beads Disperse to a single spore suspension and collect the single spore suspension for later use.

[0044] Add an equal volume of monospore suspension and 1% lithium chloride solution into a sterile test tube, mix well, let stand for 3 hours, and finally dilute 100 times with sterile phosphate buffer to terminate the reaction.

[0045] Draw 1 mL of the spore dilution treated with lithium chloride and inject it into a sterile small dish, and place it under laser irradiation. The irradiation time is 10 min. Laser wavelength: 632.8 nm, and the irradiation power density is 17.6 mw / cm2.

[0046] After the mut...

Embodiment 2

[0048] Production of Lovastatin by Liquid Fermentation of Monascus

[0049] Fermentation strain: Monascus purpureus

[0050] Seed medium:

[0051] Rice flour 3 g, glucose 2 g, NaNO 3 0.2 g, peptone 1.5 g, MgSO 4 ·7H 2 O 0.05 g, KH 2 PO 4 0.15 g, add water to 100 mL.

[0052] Fermentation medium:

[0053] Rice flour 7 g, glycerin 5 mL, NaNO 3 0.2 g, peptone 1.5 g, MgSO 4 ·7H 2 O 0.05 g, KH 2 PO 4 0.15 g, add water to 100 mL.

[0054] Training conditions:

[0055] Monascus spores were scraped from the plate and transferred to a Erlenmeyer flask containing 50 mL of seed medium, and cultured at 30°C for 3 days. According to the inoculation amount of 15%, the grown seed culture medium was transferred to 150 mL fermentation medium, and cultured on a shaker at 150 r / m at 30°C for 3 days, then cultured on a shaker at 150 r / m at 24°C for 20 days.

[0056] Sample processing and testing:

[0057] After the fermentation broth was centrifuged at 10,000 g for 10 min, 2...

Embodiment 3

[0060] Production of lovastatin by liquid state fermentation of monascus after mutagenesis of the present invention

[0061] Fermentation strain: Monascus purple

[0062] Seed Medium:

[0063] Rice flour 3 g, glucose 2 g, NaNO 3 0.2 g, peptone 1.5 g, MgSO 4 ·7H 2 O 0.05 g, KH 2 PO 4 0.15 g, add water to 100 mL.

[0064] Fermentation medium:

[0065] Rice flour 7 g, glycerin 5 mL, NaNO 3 0.2 g, peptone 1.5 g, MgSO 4 ·7H 2 O 0.05 g, KH 2 PO 4 0.15 g, add water to 100 mL.

[0066] Training conditions:

[0067] The spores of Monascus MPB3 were scraped from the plate and transferred to a Erlenmeyer flask containing 50 mL of seed medium, and cultured at 30°C for 3 days. According to the inoculation amount of 15%, the grown seed culture medium was transferred to 150 mL fermentation medium, and cultured on a shaker at 150 r / m at 30°C for 3 days, and then cultured on a shaker at 150 r / m at 24°C for 20 days.

[0068] Sample processing and testing:

[0069] After th...

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Abstract

The invention belongs to the technical field of microbes, and particularly relates to a high-yield production method of lovastatin and monascus used by the production method. The production method comprises the following steps: strain mutagenesis and fermentation by using the mutagenesis strain. Compared with the conventional monascus production strain and production method, the mutagenesis strain and method for producing lovastatin by using the mutagenesis strain have the following advantages: the lovastatin yield can reach 1650 mg/L above, the lovastatin productivity is higher, and the lovastatin quality is improved.

Description

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Claims

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Application Information

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Owner 山东中惠生物科技股份有限公司
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