42 results about "Monascus sp." patented technology

The invention belongs to the technical field of microbial fermentation engineering, and provides a monascus immunity enhancing effect fermentation optimization system and method based on data analysis, and the method comprises the following steps: a multi-source data edge preprocessing module deploys a sensor to collect dissolved oxygen, temperature and other data, and performs edge calculation filtering normalization to obtain an edge data preprocessing module; the thallus oxygen uptake rate prediction model training module establishes an oxygen uptake rate prediction model based on historical data by using LSTM in combination with an attention mechanism, and RMSE is less than 5%; the dissolved oxygen dynamic control strategy module fuses MPC and fuzzy control, the ventilation capacity and the stirring speed are dynamically adjusted according to predicted oxygen consumption, the control period is 30 seconds, an execution mechanism and a feedback correction module execute control through a mass flow controller and the like, and model parameters are corrected according to DO deviation; the whole-process closed-loop optimization module is provided with dual early warning, control parameters are updated based on a Bayesian optimization algorithm, the system achieves accurate control over dissolved oxygen in the fermentation process, and the monascus biomass and the yield of active ingredients are increased.

The invention belongs to the technical field of microorganisms, and particularly relates to a monascus capable of resisting seawater and application of the monascus in high-yield protein. According to the specific technical scheme, the monascus purpureus HXJ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the monascus purpureus HXJ5 is CGMCC No.42056, and the preservation date of the monascus purpureus HXJ5 is July 14, 2025. The content of protein in the monascus purpureus is as high as 37%. And the monascus purpureus can tolerate salt with the concentration of 15%, and can normally grow in a high-salt environment and continuously produce the microbial protein at high yield.

The invention provides monascus purpureus and application thereof. The invention provides monascus purpureus XK-G-1 which is preserved in the China Center for Type Culture Collection, and the preservation number of the monascus purpureus XK-G-1 is CCTCC (China Center for Type Culture Collection) NO: M20232397. The invention also provides a leavening agent which contains the monascus purpureus. The invention further provides red yeast rice decoction pieces and red yeast rice, the red yeast rice decoction pieces and the red yeast rice are prepared from the leavening agent, and the content of ring-opening lovastatin in the red yeast rice decoction pieces is 15-17mg / g; the total color value of the red yeast rice is 3655-3855 u / g, and the color value of the orange pigment is 1300-1426 u / g. The content of ring-opening lovastatin in the red yeast rice decoction pieces prepared by the method is stabilized at 15-17mg / g, citrinin is not detected, and the red yeast rice decoction pieces are stable in quality and safe. The monascus purpureus and the leavening agent thereof are suitable for various fermentation substrates and compositions thereof such as rice, # imgabs0 oat, highland barley, maize and sticky rice, the fermentation substrates are wide, and a new choice is provided for high added value of agricultural and sideline products.

The present invention relates to a preparation method for a monascus polysaccharide, comprising: mycelium preparation: activating a monascus strain, then carrying out inoculation to obtain a monascus seed solution, further fermenting the monascus seed solution, collecting mycelia, and drying the mycelia for later use, wherein the monascus strain is monascus pilosus, and the strain number of monascus pilosus is CICC 5045; crude polysaccharide extraction: mixing the dried mycelia with alkaline liquor, then carrying out leaching to obtain a leaching solution, carrying out ultrafiltration concentration on the leaching solution to obtain a concentrated solution, and using a sevag reagent to carry out deproteinization on the concentrated solution so as to obtain a monascus crude polysaccharide; preliminary purification: using a DEAE-52 cellulose chromatography column to carry out preliminary separation and purification on the monascus crude polysaccharide so as to obtain a polysaccharide solution; and secondary purification: using a Sephadex G-100 glucan gel chromatography column to further carry out separation and purification on the polysaccharide solution so as to obtain a monascus polysaccharide product. The purity of the monascus polysaccharide in the product prepared by the present invention reaches 95.23±3.66%, and the product has good economic value.

The invention relates to the technical field of microbial fermentation engineering, and particularly discloses natto red yeast rice herb bean flour and a preparation method thereof. The preparation method comprises the following steps: respectively activating specifically preserved bacillus natto and monascus to obtain secondary seed solutions; inoculating the two seed solutions into a solid-state fermentation culture medium for co-fermentation; and inactivating, drying and crushing the fermentation product to obtain a finished product. The solid-state fermentation culture medium is prepared by compounding a bean matrix (soybeans, black beans or chickpeas) and herb tea components (roses, lotus leaves, chrysanthemums, mulberry leaves, haws, cassia seeds and the like). Through the synergistic effect of the bacillus natto and the monascus, nattokinase and monacolin K are efficiently enriched in a single fermentation system at the same time, conversion of soy isoflavone to a high-bioavailability aglycone type is remarkably promoted, and the obtained product has multiple health-care functions of thrombolysis, lipid reduction, efficient absorption and the like.

The invention discloses a fungal preparation for improving the fermentation performance of monascus purpureus and application of the fungal preparation. The fungus preparation is prepared by compounding Aspergillus niger MQM20136, Monascus purpureus 40269 and Saccharomyces cerevisiae JH301 direct vat set fungicide, the Aspergillus niger MQM20136 is preserved in the China Center for Type Culture Collection in Wuhan, the preservation time is September 25, 2025, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20252120. According to the invention, a fungus preparation taking the aspergillus niger MQM20136 as a main body is constructed, a mutualism and synergistic interaction microbial system is formed in the fungus preparation, and when the fungus preparation is used for pure compound preparation of the monascus purpureus, the quality stability, the esterification capacity, the wine yield and other fermentation properties of the monascus purpureus can be improved.

The invention discloses a method for increasing the yield of monascus pigment through liquid fermentation, the monascus pigment is obtained through spore culture, seed culture, liquid fermentation and monascus pigment extraction by taking monascus as a production strain, and macroporous resin is added into a fermentation culture medium. According to the method, the yield of liquid fermentation of the monascus pigment can be greatly increased, the monascus pigment is concentrated in precipitates, namely resin and hyphae after solid-liquid separation is carried out on the fermentation product, a water phase of fermentation liquor does not contain pigment, separation and extraction of downstream fermentation products and treatment of fermentation waste liquor are facilitated, and the method has good market prospects and market value.

The invention relates to the field of biological fermentation, and discloses a preparation method of a fingered citron monascus fermentation composition, which comprises the following steps: 1) removing essential oil components in fingered citron in a drying mode; (2) preparing the fingered citron obtained in the step (1) and rice into powder; (3) mixing fingered citron powder, rice flour and water to prepare a solid-state fermentation substrate; 4, the monascus is inoculated into the solid-state fermentation substrate for fermentation, and the fermentation product is the fingered citron and monascus fermented composition.The monascus is used for solid-state fermentation with fingered citron and rice flour as raw materials for the first time, it is found that fingered citron can promote and increase the content of effective components including polysaccharide, Monacolin K, flavone and the like in the fermentation product, and therefore the effect of the fingered citron and monascus fermented composition is improved.

The invention provides application of RHPS4 in inhibiting monascus to produce citrinin, a biological agent and application of the biological agent, and belongs to the technical field of microbial fermentation. The invention discloses an application of RHPS4 in inhibiting production of citrinin from monascus, the RHPS4 is named as 3, 11-difluoro-6, 8, 13-trimethyl-8H-quinolo [4, 3, 2-kl] acridine onium methyl sulfate salt, the RHPS4 can reduce citrinin synthesized in the growth process of monascus, when co-fermentation culture is carried out to the tenth day, the concentration of the RHPS4 is 5 [mu] mol / L, detection finds that the citrinin is reduced by 99.7%, and the content of citrinin is reduced by 99.7%. The synthesis of citrinin is effectively inhibited. According to the method, RHPS4 and monascus are co-cultured, so that the content of citrinin is reduced, and popularization and application of monascus products are promoted.

The present invention discloses the application of Monascus purpureus and selenium-enriched Monascus purpureus prepared therefrom, belonging to the technical field of microbiology. The provided Monascus purpureus 1322 is taxonomically named Monascus purpureus 1322, and was deposited at the China Center for Type Culture Collection on August 9, 2024. The deposit address is Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, and the deposit number is: CCTCC NO: M20241767. The method for preparing selenium-enriched Monascus purpureus: inoculate the spore suspension of Monascus purpureus 1322 into a medium containing sodium selenite and culture to obtain selenium-enriched Monascus purpureus. The application of selenium-enriched Monascus purpureus in the preparation of products with lipid-lowering efficacy is provided. The present invention ferments with Monascus purpureus 1322 with strong selenium tolerance, and the obtained selenium-enriched Monascus purpureus has a significant effect of reducing hyperlipidemia.

The invention relates to the field of food preparation, in particular to a quality control method of fried monascus, which comprises the following steps: estimating the growth situation of monascus according to character data of monascus in a culture medium so as to adjust activation culture conditions; obtaining biological characteristics of monascus in the culture medium, and carrying out subregion calibration on the culture medium, so as to extract monascus and prepare a monascus strain solution; curing the pretreated rice raw material to obtain an inactivated raw material, and adjusting the inoculation operation of the inactivated raw material according to the internal structure of the inactivated raw material; predicting the fermentation trend according to the fermentation actual condition data of the inactivated raw material so as to adjust the fermentation condition, and screening the fermentation product to obtain a monascus product; and performing adjustable stir-frying operation on the red yeast rice product to obtain the processed red yeast rice. According to the method, the whole process from monascus culture to monascus extraction and strain liquid preparation is optimized, the purity of the monascus strain liquid is improved, the monascus production period is shortened, and a raw material with reliable and stable quality is provided for improving the monascus production quality.

The present application discloses a method, system and equipment for food quality detection based on Monascus fermentation, which relates to the field of microbial technology. The method comprises: performing pre-treatment operations of washing, crushing and homogenizing food samples collected based on an efficiency model to obtain target detection substances; performing detection of pollutants and active ingredients on the target detection substances to generate detection data; and performing integrated analysis on the detection data to obtain quality detection results. Through the scheme of the present application, the detection quality can be improved and detailed analysis results can be provided.

The main purpose of the present invention is to provide a Monascus pilosus and a composition for reducing weight and improving intestinal flora, the composition comprising the Monascus pilosus and / or a monascus fermentation product thereof, or a functional component comprising the monascus fermentation product. The invention also aims to provide application of the monascus in preparation of the composition for reducing weight and improving intestinal flora. The composition disclosed by the invention has the capabilities of reducing body weight and improving intestinal flora, such as reducing the ratio of the phylum firmicutes to the phylum Bacteroides.

The invention provides a method for improving the capacity of an extreme monascus sorghum fermentation product. The method comprises the following steps: S1, liquefying; s2, acidification; s3, fermentation: adding an inoculation culture medium containing monascus purpureus YX-1125 spores into the residual solid phase, carrying out immobilized culture for 24 hours by adopting a reactor which is provided with 60 matrix units to form a honeycomb structure, replacing the inoculation culture medium with a collected liquid phase, continuing to culture for 90-100 hours at 35 DEG C, collecting fermentation liquor, and filtering through a 1000Da nanofiltration membrane to obtain a fermentation product. According to the method, the influence of monascus and sorghum fermentation products on resistance to ultraviolet induced damage is greatly improved based on an acid stress means, and the method has a great promotion effect on research on the anti-inflammatory and anti-light aging potential of polar bacteria fermentation products and industrial application of acid stress in the fermentation process; the obtained product has excellent skin anti-inflammatory and anti-photoaging effects, has the potential to become a functional cosmetic raw material, and is used for protecting the skin from environmental stress.

The main purpose of the present invention is to provide monascus and a composition for preventing or treating non-alcoholic fatty liver disease, the composition comprising the monascus and / or a monascus fermentation product thereof, or a functional component comprising the monascus fermentation product. The invention also aims to provide application of the monascus in preparation of the composition for preventing or treating the non-alcoholic fatty liver disease. The composition not only has the efficacy of reducing accumulation of total cholesterol (TC) and triglyceride (TG) in the non-alcoholic fatty liver disease caused by obesity, but also has the efficacy of reducing serum liver function indexes of asparaginic acid transaminase (AST) and alanine transaminase (ALT), so that the composition has the efficacy of preventing or treating the non-alcoholic fatty liver disease and related metabolic diseases thereof. , for example hyperlipidemia.

The invention discloses a detection method of a monacolin compound. According to the application, a non-targeted screening thought is adopted, four key daughter ions of the monacolin compound are found for the first time, and non-targeted screening methods of the monacolin compound based on key daughter ion detection are respectively established in a high-resolution mass spectrum and a triple quadrupole mass spectrum by utilizing the property that the compound is easy to crack in a source in a mass spectrum; the distribution and the content of the monacolin compound can be exclusively presented. Compared with a basic analysis method of original substances, the method has the advantages that secondary mass spectrum analysis is not needed, the retention time of the compounds can be quickly locked, and the monacolin compounds are subjected to non-targeted screening; compared with the original analysis method of the monacolin compound, the method can filter out a large part of interference of other compounds, and specifically shows the distribution and content of the monacolin compound. The method can be applied to fermentation products of monascus, aspergillus terreus and other monacolin compound producing strains and products of the fermentation products to detect distribution and relative content of the monacolin compounds, and can also be applied to quality control methods such as establishment of monacolin compound fingerprints, content determination and the like in the matrix.

The invention belongs to the field of microorganisms, and particularly relates to a construction method and application of genetically engineered bacteria for super-high yield of monascus pigment. The invention discloses a construction method and application of a genetically engineered bacterium with superhigh yield of monascus pigment. The strain has the characteristic of superhigh yield of monascus pigment, and is obtained by taking red monascus as an original strain and screening by knocking out a gene MpSte11A. The invention discloses an application of the genetically engineered bacterium in microbial fermentation preparation of monascus pigment, and a liquid fermentation result shows that the highest intracellular total pigment content of MpSte11A on the 15th day is 112U / mL, which is 22 times of the yield (5.45 U / mL) of an original strain. The recombinant strain obviously promotes the production of monascus pigment under a liquid state fermentation condition, improves the fermentation efficiency, and has good commercial and scientific research values.

The invention belongs to the field of microorganisms, and particularly relates to monascus for high-yield fungus substitute protein and a construction method of the monascus. In order to improve the bacterial protein yield of monascus, the key gene nitric oxide synthetase-MrNOSL responsible for nitric oxide synthesis in monascus is locked from the perspective of genetic breeding. The inventor finds for the first time that the upstream sequence of the nitric oxide synthase mrnosl gene is replaced, so that an original promoter cannot work, namely the expression level of the mrnosl gene can be greatly improved, the content of nitric oxide synthase in monascus is increased, and synthesis of protein is promoted.

The present application belongs to the technical field of fermented food preparation, and particularly relates to a preparation method of red rice Euryale ferox powder and application thereof. The method comprises the following steps: inoculating Monascus into a PDA slant, culturing until the slant is covered with mycelium and spores, washing out the spores with sterile water after the culture is completed to obtain a spore solution; soaking dry Euryale ferox to a state capable of being kneaded, drying and sterilizing to obtain Euryale ferox material; inoculating the spore solution into the Euryale ferox material, and then performing fermentation; drying the fermentation product after the fermentation is completed to obtain red rice Euryale ferox, and screening after crushing to obtain the red rice Euryale ferox powder. The present application provides a preparation method of red rice Euryale ferox powder taking Euryale ferox as a new type of fermentation substrate, and successfully develops a high-value-added new product of deep processing of Euryale ferox. Through Monascus fermentation, two types of active ingredients of red rice and Euryale ferox are enriched, and a synergistic effect possibly generated is explored, thereby creating a compound functional food.

The invention relates to a monascus leavening of cinnamon and application thereof. The leavening is obtained by culturing monascus in a culture medium containing cinnamon or an extract thereof. The invention further relates to a food composition comprising said ferment, and to the use of said ferment for preventing microbial growth and / or maintaining humidity in food products.

The present invention belongs to the technical field of microbial fermentation engineering, and provides a fermentation optimization system and method for enhancing the immune efficacy of Monascus based on data analysis, comprising: a multi-source data edge preprocessing module deploys sensors to collect data such as dissolved oxygen and temperature, which is normalized through edge computing filtering; a bacterial oxygen consumption rate prediction model training module utilizes LSTM combined with an attention mechanism to establish an oxygen consumption rate prediction model based on historical data, with RMSE <5%; a dissolved oxygen dynamic control strategy module integrates MPC and fuzzy control, dynamically adjusts ventilation volume and stirring rate according to predicted oxygen consumption, with a control cycle of 30 seconds; an actuator and feedback correction module executes control through a mass flow controller, etc., and corrects model parameters according to DO deviation; a full-process closed-loop optimization module sets dual warnings, and updates control parameters based on a Bayesian optimization algorithm, so that the system realizes precise control of dissolved oxygen during the fermentation process and improves the biomass and active ingredient yield of Monascus.

The utility model relates to a fermentation device of monascus for producing broiler feed, which belongs to the technical field of monascus fermentation and comprises a heating barrel, a base plate is fixedly mounted on the inner bottom wall of the heating barrel, a fermentation liner is placed at the top of the base plate, a processing plate is placed at the top of the fermentation liner, and the processing plate is fixedly mounted on the inner bottom wall of the heating barrel. A hollow groove pressing head is fixedly installed at the bottom of the processing plate, and a hole opening assembly is arranged on the inclined face of the hollow groove pressing head in a penetrating mode. According to the monascus fermentation device for producing the broiler feed, a hollow groove pressing head is arranged at the bottom of a treatment plate, the hollow groove pressing head is placed in a fermentation inner container containing a substrate, the treatment plate is pressed, a groove is formed in the top of the substrate through the hollow groove pressing head, then a screw rod is pressed downwards, a top block is driven to press downwards, and a top plate of a hole opening assembly moves outwards; the hole pricking column extends outwards, uniform holes are formed in the surface of the groove of the substrate, and the problems that the holes are not uniform and the efficiency is low due to the fact that workers manually form the groove and prick some holes in the substrate are solved.

The invention belongs to the field of microorganisms, and particularly relates to monascus purpureus with high yield of essential amino acids as well as a screening method and application thereof. The invention provides a novel trans-membrane differentiation ARTP mutagenesis method aiming at filamentous fungi, aiming at the characteristics of multiple cores and thick walls of monascus, protoplast pretreatment and repair liquid components before mutagenesis are optimized, so that the fatality rate and the positive mutation rate are optimally balanced, and the problem of high instability of monascus heredity caused by conventional ARTP is solved. Based on the novel mutagenesis method, the mutant strain AZA5 is obtained through successful mutagenesis screening. The essential amino acid content of AZA5 is as high as 58%. The invention not only provides an excellent strain resource for industrial production of high-quality microbial protein, but also has important scientific value and wide industrial application potential.

The invention provides monascus purpureus, a high-yield lovastatin and low-citrinin culture medium and a method, and belongs to the technical field of microbial fermentation. The method comprises the following steps: performing streak inoculation on Monascus purpureus HBSDYX-026 to a seed culture medium, and culturing to obtain a seed solution; and inoculating the seed solution to a high-yield lovastatin culture medium for fermentation. According to the invention, by optimizing the components of the culture medium, adjusting the variety and proportion of key nutrient substances, namely a carbon source and a nitrogen source, and screening and adding traditional Chinese medicines and an accelerant, a more suitable environment is provided for the growth of monascus and the synthesis of lovastatin; a monascus strain which is stable in heredity, vigorous in growth, high in lovastatin yield and low in citrinin yield is obtained, a culture medium which can efficiently synthesize lovastatin and is low in citrinin is successfully constructed, and the yield of lovastatin in red yeast rice is remarkably increased.

The invention discloses a monascus MpigI gene interference strain as well as a construction method and application thereof, and relates to the technical field of molecular biology, the monascus MpigI gene interference strain is preserved in China Center for Type Culture Collection on November 20, 2025, and the preservation number is CCTCC NO: M 20252614. According to the present invention, the influence of the transcriptional regulation factor in the MPs synthesis gene cluster on the secondary metabolism of fungi is utilized to construct the monascus ruber Ii4 interfering the monascus ruber, the preservation number of the monascus ruber Ii4 is CCTCC NO: M 20252614, and compared with the initial strain monascus ruber M7 (CCAM 070120), the mutant strain can secrete a large amount of monascus ruber, orange pigment and yellow pigment, the intracellular haematochrome yield increase speed of the mutant strain of the red monascus Ii4 is fastest, and is about 3-5 times of the intracellular haematochrome yield of M7.

The present invention proposes an application of RHPS4 in the production of red pigment by fermentation of Monascus ruber and the application of Monascus fermentation liquid, and relates to the field of microbial fermentation technology, wherein the RHPS4 is 3,11-difluoro-6,8,13-trimethyl-8H-quinoline and [4,3,2-kl] acridinium methyl sulfate. The technical scheme of the present invention co-cultivates RHPS4 with Monascus ruber, and finds that RHPS4 can promote the increase of the unit yield of Monascus ruber through the determination of biomass and Monascus ruber content. When cultured to the 10th day, at a concentration of 2µmol / L, the biomass of Monascus ruber decreased by 49.9%, while the yellow pigment of Monascus ruber increased by 260.5 U / mL in the unit yield, the orange pigment of Monascus ruber increased by 130.3 U / mL in the unit yield, and the red pigment of Monascus ruber increased by 97.2 U / mL in the unit yield.

The present invention provides a method for increasing the yield of yellow pigment in the liquid fermentation of yam powder with Monascus spp., and belongs to the technical field of microbial fermentation. The present invention provides the application of flavonoid compounds in increasing the yield of yellow pigment in Monascus fermentation, wherein the flavonoid compounds include luteolin and / or genistein. The present invention adds flavonoid compounds to the liquid fermentation medium of Monascus spp., and after the liquid fermentation of Monascus spp., the yield of yellow pigment is significantly increased. Therefore, the method provided by the present invention realizes the maximization of flavonoid resource utilization while improving the yellow pigment production efficiency, and has important application value.

The invention discloses a natto fermentation device for promoting the solid-state fermentation of Monascus, comprising a heating source located in a box body, a supporting member, a rotating member and a lifting mechanism are arranged in the box body, the supporting member comprises a plurality of supporting plates and a plurality of supporting rods, each supporting plate corresponds to each supporting rod one by one, the supporting plate is connected to the box body, the supporting rod is coaxially arranged with the supporting plate and is slidably connected with the supporting plate, the rotating member comprises a rotating plate, a rotating shaft and a rotating motor, the rotating plate is located at the bottom of the supporting rod and is connected with the supporting rod, a driving member for driving the supporting rod to rotate is arranged on the rotating plate, the rotating shaft is located at the bottom of the rotating plate and is connected with the rotating plate, the rotating motor is located at the bottom of the box body, and the output shaft of the rotating motor is coaxially connected with the rotating shaft; the invention has a simple structure and a reasonable design, and the distance between the natto on the supporting plate and the heating source is adjusted by the rotating member, the lifting mechanism and the driving member, so that the natto is evenly fermented, thereby improving the processing quality of the natto.

This invention discloses a Monascus purpureus strain that produces high levels of Monascus pigment and low levels of citrinin, and its applications. The Monascus purpureus strain producing high levels of Monascus pigment and low levels of citrinin is classified as *Monascus purpureus* FAAS-M34 (…). Monascus purpureus The purple Monascus strain FAAS-M34 has been deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 2022959 and a deposit date of June 24, 2022. This strain exhibits a strong ability to produce natural red yeast rice pigment and low production of citrinin, making it suitable for preparing high-quality red yeast rice. The resulting red yeast rice product has a high color value, and the citrinin content does not exceed relevant national regulations, with a high product yield. Therefore, the purple Monascus strain FAAS-M34 can be safely used in the food processing industry. Compared with existing strains of the same type, it can produce higher-quality red yeast rice products and has high industrial application value.

The invention provides a special monascus culture medium capable of resisting infectious microbe pollution and a preparation method of the special monascus culture medium, and relates to the technical field. Comprising the following raw materials in parts by weight: 50 to 70 parts of rice flour, 20 to 30 parts of corn flour, 10 to 20 parts of soybean meal, 10 to 15 parts of bran, 5 to 10 parts of glucose, 3 to 5 parts of peptone, 2 to 4 parts of yeast extract, 0.5 to 1 part of monopotassium phosphate, 0.3 to 0.5 part of magnesium sulfate, 0.2 to 0.4 part of sodium chloride, 0.1 to 0.3 part of potassium sorbate and 500 to 800 parts of water. Infectious microbes in the culture medium can be effectively killed, and the sterile state of the culture medium is ensured; other ingredients are added at a proper temperature, so that the damage of high temperature to nutritional ingredients and infectious microbe resistant agents is avoided; the pH value is adjusted to a range suitable for the growth of the monascus, so that the growth and reproduction of the monascus are facilitated.