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Method for promoting monascus to produce monascus yellow pigment by adding alcohol substance

A technology of monascus yellow pigment and monascus, applied in microorganism-based methods, adding compounds to stimulate growth, biochemical equipment and methods, etc., can solve complex post-processing steps, difficult to reuse, and high cost of extractants problems, achieve significant economic benefits, do not pollute the environment, and improve the effect of synthesis

Active Publication Date: 2020-06-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the synthesis pathway of Monascus yellow pigment produced by fermentation of Monascus has not been studied in depth. It is difficult to achieve the purpose of high yield of Monascus yellow pigment by using common liquid fermentation medium components
Although in-situ extraction and fermentation are effective, the cost of the extraction agent is high, and it is difficult to reuse, and the post-processing steps are complicated

Method used

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  • Method for promoting monascus to produce monascus yellow pigment by adding alcohol substance
  • Method for promoting monascus to produce monascus yellow pigment by adding alcohol substance
  • Method for promoting monascus to produce monascus yellow pigment by adding alcohol substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Fermentative production of pigments by different bacterial strains

[0027] Seed liquid medium (g / L): cornstarch 60, ammonium sulfate 4, sodium nitrate 2, magnesium sulfate 0.5, potassium dihydrogen phosphate 2, dipotassium hydrogen phosphate 2, calcium chloride 0.1.

[0028] Fermentation medium (g / L): corn starch 60, ammonium sulfate 4, sodium nitrate 2, magnesium sulfate 0.5, potassium dihydrogen phosphate 2, dipotassium hydrogen phosphate 2, calcium chloride 0.1.

[0029] (1) Preparation of seed solution

[0030] Select Monascus purple sjs-6 (disclosed in the paper titled "Efficient Biosynthesis of Natural Yellow Pigments by Monascus purpureus in a Novel Integrated Fermentation System"), Monascus purple CICC 40225, Monascus purple CICC 40937, Monascus purple Bacteria CICC5042, and Monascus ruberus CGMCC 3.4701, Monascus ruberus CGMCC 3.4652, Monascus ruberus CGMCC 3.465, Monascus ruberus CGMCC 3.4649 are shake flask strains, after activation, they are ...

Embodiment 2

[0036] Embodiment 2: Adding different alcohols to ferment and produce pigments

[0037] Seed liquid medium (g / L): cornstarch 60, ammonium sulfate 4, sodium nitrate 2, magnesium sulfate 0.5, potassium dihydrogen phosphate 2, dipotassium hydrogen phosphate 2, calcium chloride 0.1.

[0038] Fermentation medium (g / L): corn starch 60, ammonium sulfate 4, sodium nitrate 2, magnesium sulfate 0.5, potassium dihydrogen phosphate 2, dipotassium hydrogen phosphate 2, calcium chloride 0.1.

[0039] (1) Preparation of seed solution

[0040] Monascus purple sjs-6 was selected as the shake flask strain after activation and inoculated into the seed liquid medium. Under the condition of rotation speed of 180r / min and temperature of 30°C, shake flask fermentation culture was carried out for 2 days.

[0041] (2) Fermentation culture

[0042]The Monascus liquid seeds in the logarithmic growth phase were inserted into a 500mL Erlenmeyer flask containing 50mL of fermentation medium at an inoculum...

Embodiment 3

[0045] Example 3: Pigment production by fermentation with different additions of ethanol

[0046] Seed liquid medium (g / L): cornstarch 60, ammonium sulfate 4, sodium nitrate 2, magnesium sulfate 0.5, potassium dihydrogen phosphate 2, dipotassium hydrogen phosphate 2, calcium chloride 0.1.

[0047] Fermentation medium (g / L): corn starch 60, ammonium sulfate 4, sodium nitrate 2, magnesium sulfate 0.5, potassium dihydrogen phosphate 2, dipotassium hydrogen phosphate 2, calcium chloride 0.1.

[0048] (1) Preparation of seed solution

[0049] Monascus purple sjs-6 was selected as the shake flask strain after activation and inoculated into the seed liquid medium. Under the condition of rotation speed of 180r / min and temperature of 30°C, shake flask fermentation culture was carried out for 2 days.

[0050] (2) Fermentation culture

[0051] The Monascus liquid seeds in the logarithmic growth phase were inserted into a 500mL Erlenmeyer flask containing 50mL of fermentation medium at ...

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Abstract

The invention discloses a method for promoting monascus to produce a monascus yellow pigment by adding an alcohol substances, belonging to the technical field of microbial fermentation. According to the invention, the alcohol substance is added at the initial stage of monascus fermentation, so the increase of the amount of the monascus and the synthesis of a monascus pigment are promoted. The method is simple to operate, economical and highly-efficient, does not pollute the environment, does not change the structure of a pigment, does not add additional labor and equipment, can enhance the synthesis of the monascus yellow pigment only by lower additional investment, enables the color value of the monascus yellow pigment to reach 415 U / mL, and has great economic benefits.

Description

technical field [0001] The invention relates to a method for adding alcohol substances to promote the production of monascus yellow by monascus bacteria, and belongs to the technical field of microbial fermentation. Background technique [0002] At present, microbial fermentation and plant extraction are the two main methods for producing natural yellow pigment. Plant-derived natural yellow pigments such as gardenia yellow, safflower yellow, curcumin, corn yellow, orange peel yellow, pumpkin yellow, etc. These plant-derived pigments face limitations in various aspects during the production process. On the one hand, organic reagents are used in the extraction process, the cost is high, and the output and application are limited. On the other hand, its seasonality is very strong, and its production will lead to low utilization of equipment under the influence of seasons, resulting in waste of resources. On the contrary, the use of microbial fermentation to produce natural yel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12N1/38A23L5/46C12R1/645
CPCA23L5/46C12N1/38C12P17/181
Inventor 张薄博钱高飞
Owner JIANGNAN UNIV
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