25 results about "Apolipoproteins A" patented technology

The invention provides a cardiac targeting nano drug delivery carrier based on recombinant apolipoprotein, a system, a preparation method and application. The cardiac targeting nano drug delivery carrier comprises a nano carrier core and a functional layer covalently coupled to the surface of the nano carrier core, the nano carrier core comprises an amphiphilic copolymer formed by polylactic acid-glycolic acid and polyethylene glycol; the functional layer comprises recombinant apolipoprotein A-I and heart targeting peptide; the recombinant apolipoprotein A-I contains mutation sites E191A and F225W, and the recombinant apolipoprotein A-I contains mutation sites F225W; the amino acid sequence of the recombinant apolipoprotein A-I is as shown in SEQ ID NO: 1. The cardiac targeting nano drug delivery system based on the recombinant apolipoprotein has the functions of long circulation, cardiac targeting and coordinated regulation and control of metabolism and inflammation, and can be used for precise treatment of chronic heart failure.

The invention relates to medicine, and more particularly to experimental pharmacology, and can be used for creating pharmaceutical compositions and dosage forms for the treatment of traumatic brain injury. For the purpose of broadening the existing range of drugs for the treatment of traumatic brain injury, the use of human apolipoprotein A-I or functionally significant fragments thereof is proposed, wherein the claimed drug contains native apolipoprotein A-I obtained from human blood serum, or recombinant human apolipoprotein A-I, or a functionally significant fragment of human apoA-I. The functionally significant fragment of human apoA-I has a sequence consisting of the amino acid sequence of human apoA-I truncated at the N terminal, and more particularly the amino acid sequence of human apoA-I truncated at the N terminal by 2 amino residues (amino acid residues 3-243). The drug is provided in the form of a pharmaceutical composition containing human apolipoprotein A-I or the functionally significant fragment thereof, and a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier is water for injection or saline solution. A method for treating traumatic brain injury includes locally or parenterally administering said drug to a subject in need thereof in therapeutically effective doses.

Provided are: lipid-containing nanoparticles that replace a reconstituted high-density lipoprotein (rHDL); and a composition comprising said lipid-containing nanoparticles. This complex comprises a complex lipid and an apolipoprotein A-I-derived helix 4 (H4) peptide, wherein the H4 peptide has an amino acid sequence of YLDDFQKKWQEEMELYRQKVE (SEQ NO. 1) and has a C-terminus that may be amidated. This composition comprises a complex according to the present invention. The composition can be for cosmetic use or percutaneous absorption drug use, or for the treatment of inflammatory diseases.

The application belongs to the technical field of biological detection, and particularly relates to the use of apolipoprotein A-I in the preparation of a diagnostic reagent or system for predicting the risk of severe acute hepatitis E. The application first discloses and verifies the significant correlation between the serum apolipoprotein A-I (apoA-I) level and the adverse clinical outcomes (including severe jaundice, liver failure and death) of patients with acute hepatitis E, and establishes the risk interpretation threshold (0.675 g / L and 0.435 g / L) with clinical practical value. The application can objectively and early identify high-risk patients, and provides a powerful auxiliary tool for clinical stratified management and resource optimization.

The invention discloses a model suitable for predicting metabolic syndrome (MetS) of people with polycystic ovarian syndrome (PCOS), a construction method and application, the model is characterized in that values are respectively assigned on the basis of indexes of a plurality of predictive variables, the predictive variables of the model are calculated, and the predictive variables of the predictive variables of the model are calculated according to the predictive variables of the predictive variables of the model, so that the predictive variables of the predictive variables of the model can be calculated according to the predictive variables of the model. The predictive variable is selected from sex hormone binding globulin, acne, maternal family menoxenia incidence history, age, apolipoprotein-B / apolipoprotein-A, fasting insulin and body mass index; and fitting the PCOS with MetS prediction model by adopting Logistic regression, and obtaining the morbidity according to the prediction model. According to the scheme, by optimizing model design, the requirement for the number of indexes is lowered, so that the application difficulty is lowered while the prediction precision is improved, and the prediction efficiency is remarkably improved.

The invention discloses a novel application of emodin and a high-density lipoprotein analogue in preparation of a medicine. The high-density lipoprotein analogue comprises apolipoprotein A-1 single mimetic peptide and natural phospholipid. The invention has the advantages of long-term stability and no immunogenicity and organ toxicity risk, thereby providing a clinically convertible solution for precise treatment of nerve injury.

The invention discloses a method for identifying VOCs biomarkers in expiration of a physiologically abnormal crowd based on machine learning, and the method comprises the steps: obtaining expiration VOCs data of the physiologically abnormal crowd and a normal crowd through a PTR-TOF-MS online sampling technology, and carrying out the recognition of the VOCs biomarkers in the expiration of the physiologically abnormal crowd and the normal crowd through a propensity matching scoring method in combination with mixed factors of the physiologically abnormal crowd and the normal crowd. Hybrid factor interference of VOCs data in the crowd is eliminated, and the VOCs biomarkers in expiration of the physiologically abnormal crowd are obtained through screening; the crowds with physiological abnormality are crowds with abnormal transglutaminase, crowds with abnormal glucose and / or crowds with abnormal apolipoprotein A; the mixed factor information is age, gender, body mass index and / or living habits. And a model capable of distinguishing the physiological abnormality crowd is constructed based on the screened VOCs biomarkers in the expiration of the physiological abnormality crowd, and the physiological abnormality crowd can be distinguished quickly, noninvasively and accurately by using the model.

The invention discloses a novel application of emodin and a high-density lipoprotein analogue in preparation of a medicine for treating brain nerve injury, and the high-density lipoprotein analogue comprises apolipoprotein A-1 single mimetic peptide and natural phospholipid. The invention has the advantages of long-term stability and no immunogenicity and organ toxicity risk, thereby providing a clinically convertible solution for precise treatment of nerve injury.

Disclosed is a method for diagnosing a cancer health state, or a change in cancer health state in a patient, or for diagnosing a risk of the change or presence of a cancer in a patient, comprising determining, in a plasma sample from said patient, one or more biomarker values that corresponds to apolipoprotein A-II (ApoA2)-containing complex structures, and assigning the patient as having or not having cancer, or having or not having a change in cancer health state, or having or not having a risk of cancer based on said biomarker values, wherein said cancer is selected from the group consisting of hepatocellular carcinoma (HCC), ovarian cancer (OC), and breast cancer (BC).

Peptide identified as SEQ ID NO: 1, for use in the manufacture of a medicine for treatment of a disease involving an affection of apolipoprotein A-I (Apo-AI) or transthyretin (TTR). In particular, the peptide may be useful for treatment of a disease characterized by an impairment in lipid homeostasis or in the stability of TTR. Method of treatment of a disease involving an affection in Apo-AI or TTR, which is characterized by administration of a therapeutically effective amount of a pharmaceutical composition comprising the peptide identified as SEQ ID NO: 1.

The present invention relates to a peptide identified as SEQ ID NO: 1 for use in the manufacture of a medicament for the treatment of diseases involving impairment in apolipoprotein A-I (ApoAI) or transthyretin (TTR). In particular, the peptides may be useful for the treatment of diseases characterized by impairment in intra-lipid environmental stability or TTR stability. The invention also relates to a method of treating a disease involving impairment in ApoAI or TTR, characterized in that a therapeutically effective amount of a pharmaceutical composition comprising a peptide identified as SEQ ID NO: 1 is administered.

The invention discloses a serology-based rapid diagnosis method for juvenile tuberculosis. The invention provides an economical and efficient method for diagnosing tuberculosis of teenagers. Based on serum proteomics of clinically active teenager tuberculosis patients, a rapid tuberculosis diagnosis method based on serology is found. According to the present invention, through a variety of machine learning algorithms, the combination of the three proteins such as the apolipoprotein A1 (Apolipoprotein A-I, APOA1), the hemoglobin subunit alpha (Hemoglobin subunit alpha-1, HBA1) and the hemoglobin subunit beta (Hemoglobin subunit beta, HBB) has the highest accuracy and the highest sensitivity in the teenager tuberculosis diagnosis process, such that the combination can be adopted as the serological diagnosis marker of the teenager tuberculosis; the invention provides a new method for rapid diagnosis of juvenile tuberculosis.

The present invention relates to a method for assessing myocardial infarction comprising the steps of determining the amount of a first biomarker in a sample of a subject, said first biomarker being cMyBPC, determining the amount of a second biomarker in a sample of the subject, wherein said second biomarker is selected from the group consisting of: a BMP10-type peptide (Bone Morphogenic Protein 10-type peptide), FGF23 (Fibroblast growth factor 23), a BNP-type peptide (Brain natriuretic peptide type peptide), GDF-15 (Growth differentiation factor 15), ANG2 (Angiopoietin 2), CRP (C-reactive protein), ESM1 (endothelial cell specific molecule 1), or a lipid biomarker, such as Cholesterol, LDL (Low Density Lipoprotein) or APOAT (Apolipoprotein A-1) comparing the amounts of the biomarkers to references for said biomarkers and / or calculating a score for assessing myocardial infarction based on the amounts of the biomarkers, and assessing said subject based on the comparison and / or the calculation. The invention also relates to the use of a first biomarker being cMyBPC and a second biomarker selected from the group consisting of: a BMP10-type peptide, FGF23, a BNP-type peptide, GDF15, ANG2, CRP (C-reactive protein), ESM1, or a lipid biomarker, such as Cholesterol or LDL, or at least one detection agent for said first biomarker and at least one detection agent for said second biomarker for assessing myocardial infarction. Moreover, the invention further relates to a computer-implemented method for assessing myocardial infarction and a device and a kit for assessing myocardial infarction.

Disclosed is a method for diagnosing a cancer health state, or a change in cancer health state in a patient, or for diagnosing a risk of the change or presence of a cancer in a patient, comprising determining, in a plasma sample from said patient, one or more biomarker values that corresponds to apolipoprotein A-II (ApoA2) -containing complex structures, and assigning the patient as having or not having cancer, or having or not having a change in cancer health state, or having or not having a risk of cancer based on said biomarker values, wherein said cancer is selected from the group consisting of hepatocellular carcinoma (HCC), ovarian cancer (OC), and breast cancer (BC).

The present invention relates to a method for assessing myocardial infarction comprising the steps of determining the amount of a first biomarker in a sample from a subject, the first biomarker being cMyBPC, and determining the amount of a second biomarker in a sample from a subject, the second biomarker being BMP10-type peptide (bone morphogenetic protein 10-type peptide), FGF23 (fibroblast growth factor 23), BNP-type peptide (brain natriuretic peptide-type peptide), GDF-15 (growth differentiation factor 15), ANG2 (angiopoietin-type peptide), and / or IL-16 (IL-16). The method comprises the steps of: determining the amount of a second biomarker selected from the group consisting of ethinyl 2), CRP (C-reactive protein), ESM1 (endothelial cell specific molecule 1), or lipid biomarkers, such as cholesterol, LDL (low density lipoprotein), or APOAT (apolipoprotein A-1); comparing the amount of the biomarker with a standard for the biomarker and / or calculating a score for assessing myocardial infarction based on the amount of the biomarker; and assessing the subject based on the comparison and / or calculation. The present invention also relates to the use of a first biomarker, cMyBPC, and a second biomarker selected from the group consisting of BMP10-type peptide, FGF23, BNP-type peptide, GDF15, ANG2, CRP (C-reactive protein), ESM1, or lipid biomarkers, such as cholesterol or LDL, or at least one detection agent for the first biomarker, and at least one detection agent for the second biomarker, for assessing myocardial infarction. Additionally, the present invention further relates to a computer implemented method for assessing myocardial infarction, as well as devices and kits for assessing myocardial infarction.

Provided herein is a sphingolipid loaded nanobiologic, comprising: an apolipoprotein A-1 (apoA-1) or a peptidomimetic of apoA-1; sphingolipid; the present invention relates to a nanobiological composition, and cholesterol, and uses thereof, for example, including methods of stimulating innate immune modulation of a training immune response in a subject, and methods of treating cancer, comprising administering the nanobiological composition.

Aspects of the technology relate to a composition comprising: (i) a first RNAi agent that targets Proprotein convertase subtilisin / kexin type 9 (PCSK9) and a second RNAi agent that targets angiotensinogen (AGT); (ii) a first RNAi agent that targets PCSK9 and a third RNAi agent that targets Apolipoprotein A (LPA); (iii) a second RNAi agent that targets AGT and a third RNAI agent that targets LPA, or (iv) a first RNAi agent that targets PCSK9, a second RNAi agent that targets AGT, and the third RNAi agent that targets LPA.

The invention relates to the field of medicine, in particular to experimental pharmacology, and can be used for preparing a pharmaceutical composition and a dosage form for treating craniocerebral injury. In order to expand the reserve of treatment means for craniocerebral injury, the invention provides application of human apolipoprotein A-I or functional important fragments thereof, and the means comprise natural apolipoprotein A-I (extracted from human serum) extracted from human serum, and functional fragments of recombinant human apolipoprotein A-I or human ApoA-I. The functional fragment ApoA-I has a human ApoA-I amino acid sequence truncated from an N terminal, and specifically, the human ApoA-I amino acid sequence has two amino acid residues (amino acid residues 3-243) truncated from the N terminal. The preparation is prepared in the form of a pharmaceutical composition, and comprises human serum lipoprotein AI or a functional fragment thereof and a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier is water for injection or normal saline. A method of treating craniocerebral injury comprises administering the formulation to a patient in need of treatment in a therapeutically effective dose in a topical or parenteral administration manner.

Disclosed herein, are peptides or fragments thereof capable of binding to oxidized lipids, lipid hydroperoxides or lipopolysaccharides, and a pharmaceutical composition containing the peptides or fragments and methods of using to enhance plasma anti-oxidant PON-1 activity, reduce the risk or ameliorating symptoms of a lipid-mediated inflammatory disease or treat a lipid disorder.

The present disclosure provides a therapeutic nanodisk, the therapeutic nanodisk comprising: a lipid-binding polypeptide; a lipid bilayer and a therapeutic agent, wherein the therapeutic agent may be of use for treating, preventing a central nervous system disease, disorder, trauma or injury; or as a diagnostic agent for diagnosing a central nervous system disease, disorder, trauma or injury. The lipid bilayer may be 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and the therapeutic agent may be a nucleic acid polymer. Further provided are methods for administration of the therapeutic nanodisk to treat, prevent or diagnose the central nervous system disease, disorder, trauma or injury and uses of such therapeutic nanodisks.

A new approach to targeting imaging agents to macrophage-rich sites of interest is disclosed. Compositions of the invention are rHDL and HDL-like liposomal compositions, protein constituents of which, apolipoproteins A-I and / or A-II or fragments thereof are used not only as structural but also as targeting agents. This is achieved by certain controlled chemical or enzymatic modification of apolipoproteins A-I or A-II or fragments thereof. Such modification converts these apolipoproteins to substrates for macrophage scavenger receptors and results in the improvement of contrast agent-(HDL / modified apolipoprotein)-particle association with macrophages and / or absorption (uptake) by macrophages when compared to that of the contrast agent-(HDL / apolipoprotein)-particle constructed with non-modified naturally occurring apo A-I. The compositions can be used for noninvasive specific in vivo molecular detection and localization of macrophage-rich sites of interest using imaging techniques such as computed tomography (CT), gamma-scintigraphy, positron emission tomography (PET), single photon emission computed tomography (SPECT), magnetic resonance imaging (MRI).

By systematically screening the protective agent of the composite quality control product, the optimal protective agent type and concentration of apolipoprotein A-I and transferrin are determined, so that the stability of the apolipoprotein A-I and the transferrin is improved, the degradation rate of the apolipoprotein A-I and the transferrin is reduced, and the composite quality control product can be stored for 4 weeks under an accelerated condition, so that the degradation rate of the apolipoprotein A-I and the transferrin is reduced. The contents of apolipoprotein A-I and transferrin are only slightly reduced; by optimizing a freeze-drying process, the appearance of the composite quality control product after freeze-drying, the stability of the composite quality control product after redissolution and the uniformity of the quality control product are improved.

The invention relates to application of apolipoprotein ApApoD2 in regulation and control of pea aphid lipid synthesis, the research finds that the ApApoD2 is specifically expressed in the epidermis of pea aphid, and after the ApApoD2 gene is effectively silenced by injecting the dsRNA of the ApApoD2, the epidermis lipid coating of the ApApoD2 gene is obviously reduced, the drying tolerance of the ApApoD2 gene is obviously reduced, and the apolipoprotein ApApoD2 can be applied to regulation and control of pea aphid lipid synthesis. By combining transcriptome and non-targeted metabolome analysis, it is found that the ApApoD2 gene regulates horny layer lipid synthesis of the pea aphid and accumulation of various nutrient substances, and the apApoD2 gene is very important for maintaining the lipid homeostasis of the body wall. By inhibiting the expression of the ApApoD2 gene, the lipid synthesis of the pea aphid can be effectively inhibited, so that the drying tolerance of the pea aphid is remarkably reduced, and a new thought and a new method are provided for green prevention and treatment of the pea aphid.

The present application relates to the biomedical field, and particularly to a clinical marker for monitoring CRS progress after CAR-T treatment and application thereof, the cytokine release syndrome (CRS) progress is judged by the expression of apolipoprotein A-1, so that effective treatment can be carried out and new treatment methods can be developed. The present application discloses APOA1 for the first time to monitor the cytokine release syndrome (CRS) progress after CAT-T treatment, and it is found through mass spectrometry and biochemical detection that APOA1 is expressed the lowest when the inflammation of the cytokine release syndrome (CRS) patient is the most serious, and with the recovery of the inflammation, its expression is also gradually recovered to the pre-treatment, and the cytokine release syndrome (CRS) progress of the patient has good consistency. These results suggest that APOA1 is an important clinical indicator for monitoring the cytokine release syndrome (CRS).

The invention relates to the technical field of biomedicine nanotechnologies, and discloses degreasing nanoparticles simulating a high-density lipoprotein structure and a preparation method thereof.The nanoparticles are formed by assembling human recombinant apolipoprotein A-I and 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphorylcholine according to the molar ratio of 90: 1-120: 1. The method comprises the following steps: incubating apolipoprotein in a polyethylene glycol molecule crowded environment to induce dominant conformation; constructing a phospholipid-supercritical carbon dioxide system, pumping protein liquid, and realizing instant self-assembly by utilizing a synergistic effect of ultrasonic cavitation and rapid pressure relief expansion; and finally, carrying out tangential flow ultrafiltration purification through a polyethersulfone membrane subjected to surface grafting hydrophilic modification. According to the process, protein aggregation and inactivation are effectively avoided, the problem of membrane pollution is solved, and the assembly efficiency, the particle size uniformity, the degreasing biological activity and the product yield of the nanoparticles are improved.