Stable liquid lipid calibrator

A technology for calibrators and liquids, applied in material inspection products, biological tests, etc., can solve the problems of long stabilization time, high calibration results, and high price, and achieve the effect of improving stability

Active Publication Date: 2014-12-10
NINGBO RUI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If you do not pay attention to use, the calibration result will be high, and the actual test results of patient samples will also affect clinical judgment.
The blood lipid calibrator developed by well-known foreign diagnostic reagent companies such as Roche Diagnostics and Randox has a long stable time, good accuracy, and strong product traceability, but the price is relatively expensive
Considering factors such as cost and operation in clinical practice, it is not very convenient for small and medium-sized hospitals to use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) The influence of different stabilizers on the stability of calibrators

[0039] Prepare the serum matrix liquid containing the test target apolipoprotein A-I and apolipoprotein B according to the following formula, add stabilizers of different concentrations into the matrix, and place it in a 37°C water bath for 16 hours, before and after the water bath. Use Ruiyuan Company to sell apolipoprotein A-I and apolipoprotein B detection kit (immunoturbidimetric method) and its calibrators, and detect the content of ApoA-I and ApoB in serum matrix fluid according to their respective instructions. Determine the change of the content to investigate the stability and analyze the effect of different stabilizers added. The four additives in Table 1 can all improve the stability of ApoA-I and ApoB in the serum matrix, and are positively correlated with the concentration of each stabilizer.

[0040] Serum matrix fluid containing ApoA-I and ApoB: pH7.4

[0041]

[0042]

[0043] Table ...

Embodiment 2

[0058] The difference between this embodiment and embodiment 1 is only: the lipid calibrator preparation part of the present invention:

[0059] The lipid calibrator is a composite calibrator containing high-value apolipoprotein A-I and apolipoprotein B. The formula is as follows:

[0060]

[0061] In this example, after the standard product is refrigerated at 2-8°C for one year, the recovery rate of apolipoprotein A-I is greater than 93%, apolipoprotein B is greater than 92%, and the calibration product contains apolipoprotein. The calibration accuracy of protein A-I and apolipoprotein B content is good, and the similar serum matrix and additives in the calibrator do not interfere with the detection of apolipoprotein A-I and apolipoprotein B.

Embodiment 3

[0063] The difference between this embodiment and embodiment 1 is only: the lipid calibrator preparation part of the present invention:

[0064] The lipid calibrator is a composite calibrator containing high-value apolipoprotein A-I and apolipoprotein B. The formula is as follows:

[0065]

[0066] In this example, after the standard product is refrigerated at 2-8°C for one year, the recovery rate of apolipoprotein A-I is greater than 93%, apolipoprotein B is greater than 92%, and the calibration product contains apolipoprotein. The calibration accuracy of protein A-I and apolipoprotein B content is good, and the similar serum matrix and additives in the calibrator do not interfere with the detection of apolipoprotein A-I and apolipoprotein B.

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Abstract

The invention discloses a stable liquid lipid calibrator which comprises 20-200mmol/L of buffer liquid, 2-10% (w/v) of protein stabilizer, 110-200mmol/L of electrolyte NaC, 5-100mmol/L of anti-oxidation synergist, 10-100g/L of sugar stabilizer, 0.09% (w/v) of preservative, 0.02-100mmpl/L of antioxidant, 0.1-5mmol/L of protease inhibitor, 2.0-3.0g/L of apolipoprotein A-I and 2-2.5g/L of apolipoprotein B. By adopting the stable liquid lipid calibrator, the stability of the apolipoprotein A-I and the apolipoprotein B is effectively improved, the apolipoprotein A-I and the apolipoprotein B can be stably frozen at 2-8 DEG C for one year and are relatively convenient and efficient to use and preserve, and the accuracy in measuring the contents of the apolipoprotein A-I and apolipoprotein B in a sample is improved.

Description

Technical field [0001] The invention relates to a stable calibrator for the determination of the content of apolipoprotein A-I and apolipoprotein B, in particular to a stable liquid lipid calibrator. Background technique [0002] In traditional clinical testing, to make the test results reliable or have a basis, there is often a standard. Taking the colorimetric determination of clinical chemistry testing as an example, three tests are often done: blank, standard, and determination. Adjust the absorbance to zero with the blank solution, read the absorbance of the measured colorimetric solution and the standard colorimetric solution, which are As and Au respectively; the concentration of the known standard solution is Cs. Within a certain range, a certain analyte concentration and absorbance are in a good proportional relationship. In order to overcome the matrix difference between the pure standard solution and the patient sample, a calibrator similar to the patient sample matr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/92
Inventor 陈媛张闻周海滨王建飞
Owner NINGBO RUI BIO TECH
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