Composite extracting agent and method for promoting production of monascus pigments by using of composite extracting agent

A compound extraction agent and monascus pigment technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve difficult problems, increase the production of monascus pigment, etc., achieve economic benefits and good application prospects , the effect of increased pigment production

Inactive Publication Date: 2018-07-06
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the existing methods such as strain screening and optimization of fermentation conditions have reached the bottleneck, and it is difficult to further increase the yield of red yeast rice pigments, and then seek a new type of fermentation metho

Method used

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  • Composite extracting agent and method for promoting production of monascus pigments by using of composite extracting agent

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0048] Example one

[0049] Slant medium: potato dextrose agar medium (PDA)

[0050] Seed liquid culture medium: glucose 20-40g / L, soybean powder 10-30g / L, glycerol 60-80g / L, peptone 5-15g / L, MgSO 4 ·7H 2 O 0.5-3g / L, KH 2 PO 4 1-4g / L, NaNO 3 1-3g / L.

[0051] Fermentation broth medium: glucose 30-60g / L, soybean powder 15-35g / L, glycerol 70-100g / L, peptone 5-20g / L, MgSO 4 ·7H 2 O 0.5-2g / L, KH 2 PO 4 1.5-5g / L, NaNO 3 1-5g / L, ZnSO 4 ·7H 2 O 1-4g / L.

[0052] Determination and calculation of the color value of the three pigments: take a certain volume of fermentation broth, centrifuge, filter, and dilute the filtrate to an appropriate multiple with deionized water, and use a spectrophotometer to measure at 390-420nm, 430-480nm, and 490-520nm wavelengths. The OD value, the OD value at the three wavelengths, multiplied by the dilution factor respectively, is the color value of the extracellular yellow, orange, and red pigments. The pellet after centrifugation was mixed with 50-90% ethanol ...

Example Embodiment

[0059] Embodiment two:

[0060] The bacteria culture and fermentation process steps are the same as in the above example 1, except that the fermentation medium is prepared according to the following formula. The basic medium is rice flour 30-60g / L, glycerol 70-100g / L, and peptone 5- 20g / L, MgSO 4 ·7H 2 O 0.5-2g / L, KH 2 PO 4 1.5-5g / L, NaNO 3 1-5g / L, ZnSO 4 ·7H 2 O 1-4g / L. The extraction phase is still nonionic surfactant Span80 and peanut oil, and the addition time is the same as in Example 1. The total color values ​​of the red, orange and yellow pigments measured after 5-10 days of fermentation under the same fermentation conditions as in Example 1 were 180-190 U / mL, 160-170 U / mL, and 180-190 U / mL, respectively.

Example Embodiment

[0061] Embodiment three:

[0062] The steps and fermentation medium formula are the same as those of the above-mentioned Example 1, except that the strains are different. The Monascus RP2 preserved in our laboratory is selected.

[0063] The fermentation steps, fermentation process and conditions are the same as in the first embodiment. After the fermentation, the total color values ​​of the red, orange, and yellow pigments measured by an ultraviolet spectrophotometer were 150-160 U / mL, 140-150 U / mL, 140-150 U / mL, respectively.

[0064] It can be seen from the above examples that the composite extractant combined with nonionic surfactant Span80 and peanut oil has good biocompatibility and pigment extraction ability for Monascus. The invention can efficiently produce monascus pigment and has important practical value and economic value in the field of monascus pigment production.

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Abstract

The invention belongs to the field of fermentation engineering, and discloses a composite extracting agent and a method for promoting production of monascus pigments by using of the composite extracting agent, wherein the method comprises the steps of: inoculating a monascus liquid seed in the logarithmic growth phase into a fermentation medium according to the inoculum size of 3%-8% by volume, adding peanut oil into the fermentation medium according to the amount of 70 mL/L fermentation liquid to 180 mL/L fermentation liquid; performing shake-flask culture at temperature of 27 DEG C to 33 DEGC; adding non-ionic surfactant Span80 into the fermentation system containing the Monascus liquid seed and the peanut oil according to the amount of 2.8 g/L fermentation liquid to 3.4 g/L fermentation liquid, performing shake-flask culture again, and the like. The final yields of red, orange and yellow pigments in the fermentation liquid with the composite extracting agent added are increased by76%, 85% and 89%, respectively, compared with the control group.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and in particular relates to a compound extractant and a method for promoting the production of monascus pigment by using the compound extractant. Background technique [0002] Monascus pigment is a safe natural pigment, which is relatively stable to acid-base, oxidant and reducing agent. It has the characteristics of good thermal stability, strong protein coloring power, soft tone, stable pH and anti-oxidation. The color agent nitrite is used in the coloring of meat products, puffed food, aquatic products and seasoning cans, so the application of monascus pigment has a huge market prospect. [0003] At present, the production method of monascus pigment is mainly liquid fermentation method, but this traditional liquid fermentation method faces two problems: the negative feedback inhibition caused by the high concentration of the product in the cell during the fermentation process; Pigment...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/645
CPCC12P1/02C12N1/145C12R2001/645
Inventor 杨雪莲曹雁平王成涛董晔
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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