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Toluidine blue O with effect of screening rhodotorula as well as application thereof

A technology of toluidine blue and red yeast, which is applied in the directions of fermentation, microbial-based methods, microbial measurement/inspection, etc., can solve the problems of cumbersome mutagenesis conditions, low efficiency, high cost, etc., and achieve low cost, high efficiency, and process simple effect

Inactive Publication Date: 2018-05-25
博益德(北京)生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ARTP mutagenesis conditions are cumbersome and costly, and the mutagenized strains are cultured in single colonies one by one under natural conditions, which is inefficient.

Method used

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  • Toluidine blue O with effect of screening rhodotorula as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Activate Rhodotorula, inoculate in yeast extract powder peptone glucose (YPD) liquid medium, and culture at 200 rpm at 28°C for 2 days as a mutagenic strain;

[0049] (2) irradiating the mutagenic strain described in step (1) under a 40W ultraviolet lamp for 5min to obtain mutagenic red yeast;

[0050] (3) Using yeast extract powder peptone glucose liquid medium added with 1mmol / mL toluidine blue O, cultivate the mutagenized Rhodotorula yeast described in step (2) at 28°C for 24h, and the rotating speed of the shaker is 200rpm;

[0051](4) Spreading the rhodotorula suspension on a solid medium and culturing at 28° C. for 4 days to obtain the screened rhodotorula.

Embodiment 2

[0053] (1) Activate Rhodotorula, inoculate in yeast extract powder peptone glucose (YPD) liquid medium, and culture at 200 rpm at 28°C for 2 days as a mutagenic strain;

[0054] (2) irradiating the mutagenic strain described in step (1) under a 40W ultraviolet lamp for 3min to obtain mutagenic red yeast;

[0055] (3) Using yeast extract powder peptone glucose liquid medium added with 1.5mmol / mL toluidine blue O, cultivate the mutagenized Rhodotorula yeast described in step (2) at 28°C for 24h, and the rotating speed of the shaker is 200rpm;

[0056] (4) Spreading the rhodotorula suspension on a solid medium and culturing at 28° C. for 4 days to obtain the screened rhodotorula.

Embodiment 3

[0058] (1) Activate Rhodotorula, inoculate in yeast extract powder peptone glucose (YPD) liquid medium, and culture at 200 rpm at 28°C for 2 days as a mutagenic strain;

[0059] (2) irradiating the mutagenic strain described in step (1) under a 40W ultraviolet lamp for 7min to obtain mutagenic red yeast;

[0060] (3) Using yeast extract powder peptone glucose liquid medium added with 0.5mmol / mL toluidine blue O, cultivate the mutagenized Rhodotorula yeast described in step (2) at 28°C for 24h, and the rotating speed of the shaking table is 200rpm;

[0061] (4) Spreading the rhodotorula suspension on a solid medium and culturing at 28° C. for 4 days to obtain the screened rhodotorula.

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Abstract

The invention provides toluidine blue O with effect of screening rhodotorula as well as application thereof. The toluidine blue O is added in a culture medium of the rhodotorula to cause an oxygen stress environment, the toluidine blue O and ultraviolet mutagenesis jointly cooperate to rapidly and effectively screen out rhodotorula capable of producing high-yield carotenoid, and important significance in the field of production of carotenoid is achieved.

Description

technical field [0001] The invention belongs to the technical field of yeast screening, and relates to a toluidine blue O capable of screening red yeast and an application thereof. Background technique [0002] Carotenoids are red, yellow or orange fat-soluble pigments that widely exist in nature, including β-carotenoids, astaxanthin, lutein and lycopene. As precursor substances, carotenoids can generate a variety of derivatives with important physiological functions, such as retinoids, plant hormones, pigments and aromatic compounds, etc., which have the functions of enhancing the body's immunity, anti-oxidation, anti-radiation, anti-tumor and therapeutic It plays an important role in photosensitivity diseases and has a wide range of applications in food, cosmetics, medicine and feed industries. [0003] The industrial production methods of carotenoids mainly include chemical synthesis, plant extraction and biological fermentation. The chemical synthesis method is complex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12N1/14C12P23/00C12R1/645
CPCC12N1/14C12P23/00C12Q1/04
Inventor 田子罡陈达王春雨
Owner 博益德(北京)生物科技有限公司
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