Method for produce monascus by using distillers' grain fermentation

A technology for distiller's grains and red yeast rice, which is applied in the field of producing red yeast rice by using distiller's grains fermentation, can solve the problems of low added value, low efficiency, and no consideration of the health-care effect of red yeast rice production process method, etc., and achieves the effects of good effect and high metabolic efficiency.

Inactive Publication Date: 2013-09-25
ZHEJIANG GUOQU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method provides a way for the utilization of distiller's grains and the recycling of liquor production, but this method is only to obtain red yeast rice with high saccharification power for winemaking, which is still used for winemaking, and the added

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of distiller's grain saccharification liquid matrix:

[0023] Grind the dried distiller's grains to 60-80 mesh, add water and mix evenly according to the weight ratio of distiller's grains to water 1:15, heat to 90°C, add 6% α-amylase, liquefy for about 30 minutes, cool down to 65°C, and then add 8 % of glucoamylase, heat preservation and saccharification for 5 hours;

[0024] (2) Configure solid-state fermentation medium:

[0025] Mix 200 g of distiller's grain saccharification solution obtained in step (1) with 350 g of rice flour, 150 g of rice bran, and 150 g of soybean flour, and then add nutrient solution (containing 20 g / L soybean peptone, 20 g / L sucrose, and 20 g / L yeast extract ), adjust the water content to 50%, mix well and divide into 500mL triangular flasks, 120g per bottle, steam sterilize, sterilize at 121°C for 50min, and then break up the solid fermentation medium materials in the bottle while it is hot;

[0026] (3) Fermentation of Mo...

Embodiment 2

[0030] (1) Preparation of distiller's grain saccharification liquid matrix:

[0031] Grind the dried distiller's grains to 60-80 mesh, add water and mix evenly according to the weight ratio of distiller's grains to water 1:15, heat to 90°C, add 6% α-amylase, liquefy for about 30 minutes, cool down to 65°C, and then add 8 % of glucoamylase, heat preservation and saccharification for 5 hours;

[0032] (2) Configure solid-state fermentation medium:

[0033] Mix 300 g of the distiller's grain saccharification solution obtained in step (1) with 350 g of rice flour, 200 g of rice bran, and 200 g of soybean flour, and then add a nutrient solution (containing 50 g / L of soybean peptone, 50 g / L of sucrose, and 20 g / L of yeast extract ), adjust the water content to 40%, mix well and divide into 500mL triangular flasks, 120g per bottle, steam sterilize, sterilize at 121°C for 50min, and then break up the solid fermentation medium materials in the bottle while it is hot;

[0034] (3) Fer...

Embodiment 3

[0037] (1) Preparation of distiller's grain saccharification liquid matrix:

[0038] Crush the dried distiller's grains to 40-60 mesh, add water and mix evenly according to the weight ratio of distiller's grains to water 1:20, heat to 90°C, add 5% α-amylase, liquefy for about 30 minutes, cool down to 55°C, and then add 9 % glucoamylase, keep warm and saccharify for 6 hours;

[0039] (2) Configure solid-state fermentation medium:

[0040] Mix 320g of distiller's grain saccharification liquid obtained in step (1) with 100g of rice flour, 150g of rice bran, and 150g of soybean flour, and then add nutrient solution (containing soybean peptone 40g / L, sucrose 40g / L, yeast extract 20g / L ), adjust the water content to 35%, mix well and divide into 500mL triangular flasks, 120g per bottle, steam sterilize, sterilize at 121°C for 50min, and then break up the solid fermentation medium materials in the bottle while it is hot;

[0041] (3) Monascus pilosus fermentation and cultivation: Mon...

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Abstract

The present invention relates to a fermentation method, particularly to a method for produce monascus by using distillers' grain fermentation. The method specifically comprises the following steps: (1) preparing a distillers' grain saccharification liquid matrix: crushing dried distillers' grains to achieve 40-80 meshes, adding water to the crushed distillers' grains according to a weight ratio of the distillers' grains to the water of 1:10-20, uniformly mixing, heating to a temperature of 85-95 DEG C, adding alpha-amylase to carry out liquefaction for 20-30 min, cooling to a temperature of 55-65 DEG C, adding glucoamylase, and carrying out thermal insulation saccharification for 4-6 h to obtain a distillers' grain saccharification liquid; (2) preparing a solid state fermentation culture medium: adopting the distillers' grain saccharification liquid obtained from the step (1) to prepare a solid state fermentation culture medium, wherein the addition amount of the distillers' grain saccharification liquid is 15-32% of the total weight of the solid state fermentation culture medium; and (3) carrying out fermentation culture on monascus sp. The monascus prepared by using the method contains a plurality of physiological activity substances, further contains monascus pigment, ergosterol, gamma-aminobutyric acid and unsaturated fatty acids, and has a good health care effect, wherein amino acid content achieves 20%, and MonacolinK content can achieve 4-30 mg/g.

Description

technical field [0001] The invention relates to a fermentation method, in particular to a method for producing red yeast rice by fermenting distiller's grains. Background technique [0002] In 2011, the total output of my country's liquor industry increased by 30.7% year-on-year, reaching 10.256 million kiloliters. The rapid development of the liquor industry has driven the rapid development of liquor production capacity, but behind the rapid increase in production, the treatment of distiller's grains after production has become a difficult problem for enterprises. [0003] Now it is common to use distiller's grains directly as feed to feed livestock, in which a large amount of protein has not been transformed and is difficult to be absorbed and utilized, resulting in a lot of waste. It is also a common mode to use distiller's grains to make high-protein feed by full drying method, but the added value is low and the investment in equipment is large. Distiller's grains are ...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/645
Inventor 甘锋唐建忠汪排岭
Owner ZHEJIANG GUOQU BIO TECH
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