Method for preparing functional minced larimichthys crocea products by using mixed microbe fermentation
A surimi product, mixed bacteria fermentation technology, applied in the field of aquatic product processing, can solve problems such as poor flavor and strong fishy smell, achieve the effects of improving technical level and product added value, simple operation, and enhancing immune function of the body
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Embodiment 1
[0023] (1) Choose fresh large yellow croaker as raw material, after dissection, remove the scales, skin, head, tail, viscera, wash away blood stains and black film, take the meat and mince it to obtain large yellow croaker surimi; the obtained surimi Add 1% salt, 3% starch, and 2% maltose by weight to the mixture, mix at 16°C for 30 minutes, and sterilize at 115°C for 20 minutes before use;
[0024] (2) Monascus purple (CICC 40937) was activated and cultured on malt juice agar slant medium for 5 days, then inserted into liquid medium for shaking flask culture, and liquid strains of Monascus were obtained;
[0025] (3) Lactobacillus paracasei (CICC 20241) was anaerobically activated and cultivated on MRS agar slant medium for 1 day, and then inserted into lactic acid bacteria liquid medium for anaerobic liquid fermentation culture to obtain lactic acid bacteria liquid strains;
[0026] (4) Use the minced surimi obtained in step (1) as the fermentation raw material, add liquid s...
Embodiment 2
[0033] (1) Choose fresh large yellow croaker as raw material, after dissection, remove the scales, skin, head, tail, viscera, wash away blood stains and black film, take the meat and mince it to obtain large yellow croaker surimi; the obtained surimi Add 0.5% salt, 1% starch, and 3% maltose by weight to the mixture, mix at 20°C for 60 minutes, and sterilize at 115°C for 20 minutes before use;
[0034] (2) Monascus sorghum (CBS 302.78) and Monascus comamosa (CICC 40710) were respectively activated and cultured on wort agar slant medium for 5 days, and then added to liquid medium at a weight ratio of 1:1 for shake flask culture , to obtain the Monascus liquid strain;
[0035] (3) Leuconostoc enterococci (CICC 20054) and Pediococcus pentosaceae (ATCC 10791) were anaerobically activated on MRS agar slant medium for 1 day, and then inserted into lactic acid bacteria liquid medium at a weight ratio of 1:1 Carry out anaerobic liquid state fermentation culture, obtain lactic acid bac...
Embodiment 3
[0043] (1) Choose fresh large yellow croaker as raw material, after dissection, remove the scales, skin, head, tail, viscera, wash away blood stains and black film, take the meat and mince it to obtain large yellow croaker surimi; the obtained surimi Add 2% salt by weight, 10% starch, and 10% maltose, chop and mix at 30°C for 40 minutes, and sterilize at 115°C for 20 minutes before use;
[0044] (2) Monascus sorghum (CBS 302.78) was activated and cultivated on a malt juice agar slant medium for 5 days, and then inserted into a liquid medium for shaking flask culture to obtain a liquid strain of Monascus;
[0045] (3) Pediococcus pentosaceae (ATCC 10791) was anaerobically activated and cultivated on MRS agar slant medium for 1 day, and then inserted into lactic acid bacteria liquid medium for anaerobic liquid fermentation culture to obtain lactic acid bacteria liquid strains;
[0046] (4) Use the minced surimi obtained in step (1) as the fermentation raw material, add liquid st...
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