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Method for producing lipid-lowering component Monacolin K of monascus by using spent grains

A technology of monascus and wheat grains, which is applied in the field of Monascus using wheat grains to produce MonacolinK, a lipid-lowering component, can solve problems such as insufficient nutrients, reduce production costs, meet green environmental protection and sustainable development, and has great research significance. and economic value

Inactive Publication Date: 2019-10-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its composition is very different from that of rice, which makes solid-state fermentation of wheat grains with Monascus bacterium very difficult, such as insufficient nutrients

Method used

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  • Method for producing lipid-lowering component Monacolin K of monascus by using spent grains
  • Method for producing lipid-lowering component Monacolin K of monascus by using spent grains
  • Method for producing lipid-lowering component Monacolin K of monascus by using spent grains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Fermentative production of Monacolin K by different bacterial strains

[0039] Seed liquid medium: glucose 60g L -1 , fish meal peptone 25g L -1 , sodium nitrate 2g·L -1 , magnesium sulfate heptahydrate 1g·L -1 , dipotassium hydrogen phosphate 1g·L -1 , corn flour 6g·L -1 , the pH was adjusted to 6.0.

[0040] Fermentation medium: wheat grains, 50% water content.

[0041] (1) Preparation of seed solution

[0042] Monascus purpureus 9901 (see Jiang Bingjie's master's degree thesis "Research on Monascus purpureus high-yield Monacolin K by solid-state fermentation", public date: 2015-12-31, in section 2.1.1 of the paper, Monascus purpureus (Monascus purpureus) was recorded 9901, and then sent the bacterium to the Chinese Academy of Sciences for identification as Monascus ruber (Monascus ruber), so it was written as Monascus ruber 9901), CICC number is 41649, CICC number is 41232 Monascus ruber, CICC number is 41601, CICC Monascus purple with the numbe...

Embodiment 2

[0047] Embodiment 2: Fermentative production of Monacolin K with different initial water contents

[0048] Fermentation medium: wheat grains, with different initial moisture contents.

[0049] The liquid seed of Monascus ruberus 9901 in the logarithmic growth phase obtained in Example 1 was inserted into a 250mL Erlenmeyer flask with 15g wheat grains at an inoculum size of 10% by volume, and the initial water content of the fermentation medium was 45% respectively. %, 50%, 55%, 60%, 65%. Under the condition of temperature 30°C, the fermentation culture is 2d; under the condition of temperature 25°C, the fermentation culture is 18d. After the fermentation, the measured Monacolin K content was 6.243mg / g, 5.149mg / g, 4.783mg / g, 2.326mg / g, 1.904mg / g.

[0050] Table 2 The results of different initial water content fermentation

[0051]

Embodiment 3

[0052] Embodiment 3: Fermentative production of Monacolin K with different inoculum sizes

[0053] Fermentation medium: 10g wheat grains, 45% water content.

[0054] The Monascus ruberus 9901 liquid seed that is in the logarithmic growth phase that obtains in embodiment 1 inserts respectively with volume ratio 5%, 10%, 15%, 20%, the 250mL that 15g grains of grain are housed In the triangular flask, the initial water content is 50%. Under the condition of temperature of 30°C, the fermentation culture was carried out for 2 days; under the condition of temperature of 25°C, the fermentation was carried out for 18 days. After the fermentation, the Monacolin K content was measured as 4.58mg / g, 6.085mg / g, 5.248mg / g, 4.306mg / g, 4.506mg / g.

[0055] Table 3 The results of fermentation with different inoculum sizes

[0056]

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Abstract

The invention discloses a method for producing a lipid-lowering component Monacolin K of monascus by using spent grains, and belongs to the technical field of antibacterial fermentation. According tothe method, solid state fermentation is conducted on the spent grains by using the monascus to produce the Monacolin K, and the yield of the Monacolin K reaches 8.704 mg / g. The spent grains are main waste in the brewage process, and are edible and harmless, the spent grains are used for monascus solid state fermentation to produce the lipid-lowering component Monacolin K, the production cost of monascus products can be lowered, the requirements of environmental protection and sustainable development are met at the same time, the situation that waste materials are changed into things of value is achieved, the significant research significance and economic value are achieved, the method can be generalized to utilization of white spirit and yellow rice wine brewing waste spent grains, and thegood economic benefits are achieved in the same way.

Description

technical field [0001] The invention relates to a method for Monacolin K, which is a lipid-lowering component produced by Monascus bacterium from wheat grains, and belongs to the technical field of microbial fermentation. Background technique [0002] Cardiovascular and cerebrovascular diseases have become one of the main killers of human health, which has an important relationship with the excessive accumulation of cholesterol in human blood. As an active substance of fungal metabolism, monacolin is a competitive inhibitor of the key enzyme HMG-CoA reductase in cholesterol synthesis, which can effectively maintain cholesterol at a low density level. In terms of inhibiting cholesterol synthesis, Monacolin K belongs to the Monacolin substances with excellent effect, but according to the existing research, Monacolin K is still mainly produced by fermentation, but its yield is low. In recent years, the morbidity and mortality of cardiovascular diseases have jumped to the first...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06C12R1/645
CPCC12P17/06
Inventor 张薄博钱高飞吕楚君陈磊管宏伟
Owner JIANGNAN UNIV
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