Functional red yeast rice prepared from germinated brown rice and method
A germinated brown rice, functional technology, applied in the field of red yeast rice fermentation
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Embodiment 1
[0032] a. Conditions for physical mutagenesis: UV mutagenesis, select and cultivate mature strains of spores to prepare a spore suspension, evenly spread in a sterile blank plate, sterile air-dried, use 25 watt UV lamp, distance 30-40 Cm, 60 seconds, its lethality rate is about 80%;
[0033] b. Separation and purification steps: the irradiated spore suspension is cultured on a gradient plate, and single colonies with obvious wrinkles, abundant aerial hyphae, and larger colonies are selected, and the production of the strain is verified by at least three fermentation tests after cultivation. The ability of the target product; after determining the target strain, use 11Be0 wort medium to purify, cultivate and isolate, cultivate at 30°C for 7 days, use slant medium to mature and store at 3°C;
[0034] c. Sprouted brown rice, soak in water for 3 hours at room temperature, then drain for 5 hours and soak for 3 hours, start to germinate, germination temperature 28℃, germination time 4-6 ...
Embodiment 2
[0037] A method for preparing functional red yeast rice from germinated brown rice includes the following steps:
[0038] a. Conditions for physical mutagenesis: UV mutagenesis, select and cultivate mature strains of spores to prepare a spore suspension, use a 25-watt UV lamp, 35 cm distance, 100 seconds, so that its lethality rate is about 85%;
[0039] b. Separation and purification steps: the irradiated spore suspension is cultured on a gradient plate, and single colonies with obvious wrinkles, abundant aerial hyphae and larger colonies are selected, and the enzyme activity is verified by at least three fermentation tests after cultivation. Select high enzyme activity strains, use 11Be0 wort culture medium to purify, culture and isolate, culture temperature 30℃, time 7 days, use slant culture medium to mature and store at 4℃;
[0040] c. Sprouted brown rice, soak in water for 3 hours at room temperature, then drain for 5 hours and soak for 3 hours, start to germinate, germinate te...
Embodiment 3
[0043] a. Conditions for physical mutagenesis: UV mutagenesis, select and cultivate mature strains of spores to make a spore suspension, spread it evenly in a sterile blank plate, sterile air-dry, use a 25-watt UV lamp at a distance of 40 cm, The time is 100 seconds, so that its fatality rate is about 85%;
[0044] b. Separation and purification steps: the irradiated spore suspension is cultured on a gradient plate, and single colonies with obvious wrinkles, abundant aerial hyphae, and larger colonies are selected, and the production of the strain is verified by at least three fermentation tests after cultivation. The ability of the target product; after determining the target strain, use 11Be0 wort medium for purification, culture and isolation, culture temperature 30℃, time 7 days, use slant medium to cultivate mature, and store at 5℃ low temperature;
[0045] c. Germinated brown rice, soak in water for 3 hours at room temperature, then drain for 5 hours and then soak for 3 hours...
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