The invention discloses an aspergillus oryzae aged cheese and a preparation method of the aspergillus oryzae aged cheese. The preparation method of the aspergillus oryzae aged cheese, disclosed by the invention, comprises the following steps: (1), inoculating a lactic acid bacteria starter and Aspergillus Oryzae spore powder on sterilized raw material milk, fermenting until the pH value is 6.3-6.5, wherein the adding amount of the Aspergillus Oryzae spore powder is 0.0001-0.0008 percent the weight of the raw material milk; (2), adding chymosin, coagulating to obtain curd; (3), cutting the curd to obtain curd blocks; and (4), removing whey from the curd blocks, then forming in a mould, curing after demoulding, and ageing, wherein the ageing time is 1-7 days at a temperature of 20-30 DEG C, the ageing time is 7-15 days at a temperature of 15-20 DEG C, and the ageing time is 10-20 days at a temperature of 8-12 DEG C continuously, and the relative humidity of an environment of the ageing is 85-95 percent. The cheese prepared by adopting the preparation method disclosed by the invention is high in nutritive values; and the preparation method is simple, convenient and easy to operate, short in production period and convenient to popularize and apply.