Red yeast rice rich in coenzyme Q10 and preparation method thereof
A technology of coenzyme and red yeast rice, applied in the field of red yeast rice, to achieve the effect of promoting synthesis, increasing content, and easy operation
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Embodiment 1
[0023] A preparation method of red yeast rice rich in coenzyme Q10 comprises the following steps:
[0024] (1) Seed liquid preparation: transfer single colonies of activated Monascus comamosa (IFO4521) and Monascus ruber (NBRC32318) to flat PDA medium for 7 days at 30°C, and then use 2wt% Tween- Gently elute the spores grown on the PDA plate with 20 sterilized water respectively, and prepare the spore suspension of Monascus comamosa and Monascus ruberus, the concentration of the spore suspension is 2.4×10 5 Each / mL, the two kinds of spore suspensions were respectively connected to the seed medium, the inoculum amount was 15wt%, and cultured on a shaking table at 30°C and 150rpm for 2 days, and then the two kinds of seed solutions were mixed in equal volumes and set aside;
[0025] The seed medium is 50mL of glycerin, 30g of glucose, 10g of yeast extract, 8g of peptone, 2g of potassium nitrate, 1g of ammonium dihydrogen phosphate, 1g of magnesium sulfate heptahydrate, 20g of ri...
Embodiment 2
[0047] A preparation method of red yeast rice rich in coenzyme Q10 comprises the following steps:
[0048] (1) Seed solution preparation: transfer single colonies of activated Monascus comamosa (IFO4521) and Monascus ruber (NBRC32318) to flat PDA medium for 8 days at 30°C, and then use 2wt% Tween-20 Gently elute the spores grown on the PDA plate with sterile water to prepare the spore suspensions of Monascus comamosa and Monascus ruberus, so that the concentration of the spore suspension is 5.6×10 5 Each / mL, the two kinds of spore suspensions were respectively connected to the seed medium, the inoculum amount was 15wt%, and cultured on a shaking table at 30°C and 150rpm for 3 days, and then the two kinds of seed solutions were mixed in equal volumes and set aside;
[0049] The seed medium is 50mL of glycerin, 30g of glucose, 10g of yeast extract, 8g of peptone, 2g of potassium nitrate, 1g of ammonium dihydrogen phosphate, 1g of magnesium sulfate heptahydrate, 20g of rice flour...
Embodiment 3
[0056] A preparation method of red yeast rice rich in coenzyme Q10 comprises the following steps:
[0057] (1) Seed solution preparation: transfer single colonies of activated Monascus comamosa (IFO4521) and Monascus ruber (NBRC32318) to flat PDA medium for 8 days at 30°C, and then use 2wt% Tween-20 Gently elute the spores grown on the PDA plate with sterile water to prepare the spore suspensions of Monascus comamosa and Monascus ruberus, so that the concentration of the spore suspension is 1.8×10 5 Each / mL, the two kinds of spore suspensions were respectively connected to the seed culture medium, the inoculum amount was 15wt%, and cultured on a shaking table at 30°C and 150rpm for 4 days, and then the two kinds of seed solutions were mixed in equal volumes and set aside;
[0058] The seed medium is 50mL of glycerin, 30g of glucose, 10g of yeast extract, 8g of peptone, 2g of potassium nitrate, 1g of ammonium dihydrogen phosphate, 1g of magnesium sulfate heptahydrate, 20g of ri...
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