Method for producing lovastatin by fermentation and fermentation medium used by same

A fermentation medium and lovastatin technology, which is applied in the field of biopharmaceuticals, can solve the problems of low lovastatin content, high raw material cost, long fermentation cycle and the like, and achieve the effects of shortening the fermentation cycle, reducing the cost of raw materials and reducing the production cost.

Inactive Publication Date: 2012-07-11
PEKING UNIV FOUNDER GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there have been no relevant reports at home and abroad on the fermentation medium that directly uses maltose as the main carbon source and does not contain glucose.
[0007] The method for producing lovastatin by fermentation with glucose as a carbon source has the following defects: the cost of raw materials is high, the content of lovastatin in the fermentation broth is low, and the fermentation cycle is long

Method used

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  • Method for producing lovastatin by fermentation and fermentation medium used by same

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Embodiment 1

[0082] In this embodiment, 5M 3 The fermentation tank is taken as an example to illustrate the process of preparing lovastatin by fermentation of the present invention.

[0083] The formula of the fermentation medium by weight / volume percentage is: 20.0% maltose, 4.0% cottonseed powder, 1.0% peptone, 0.5% solid malt extract, 0.2% sodium chloride, 0.05% potassium dihydrogen phosphate, 0.05% magnesium sulfate, defoaming Agent 0.05%, adjust the pH to 6.5.

[0084] The preparation method of fermentation medium is as follows:

[0085] 1. Add an appropriate amount of drinking water to the batching tank, then put in the raw materials (except maltose) of the formula amount, stir evenly, and pump to 5M 3 In the fermenter, the volume was adjusted to a certain volume with drinking water, and sterilized at 121°C for 30 minutes.

[0086] 2. Add an appropriate amount of drinking water to the batching tank, then put in the formula amount of maltose, stir evenly, pump it into the sugar tan...

Embodiment 2

[0090] In this embodiment, 5M 3 The fermentation tank is taken as an example to illustrate the process of preparing lovastatin by fermentation of the present invention.

[0091] The formula of the fermentation medium is: maltose 18.0%, cottonseed powder 4.0%, peptone 1.0%, solid malt extract 0.5%, sodium chloride 0.2%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, adjust pH is 6.5.

[0092] The preparation method of fermentation medium is as follows:

[0093] 1. Add an appropriate amount of drinking water to the batching tank, then put in the raw materials (except maltose) of the formula amount, stir evenly, and pump to 5M 3 In the fermenter, the volume was adjusted to a certain volume with drinking water, and sterilized at 121°C for 30 minutes.

[0094] 2. Add an appropriate amount of drinking water to the batching tank, then put in the formula amount of maltose, stir evenly, pump it into the sugar tank, use drinking water to set the volume to a certain vo...

Embodiment 3

[0098] In this embodiment, 50M 3 The fermentation tank is taken as an example to illustrate the process of preparing lovastatin by fermentation of the present invention.

[0099] The formula of the fermentation medium by weight / volume percentage is: 20.0% maltose, 4.0% cottonseed powder, 1.0% peptone, 0.5% solid malt extract, 0.2% sodium chloride, 0.05% potassium dihydrogen phosphate, 0.05% magnesium sulfate, defoaming Agent 0.05%, adjust the pH to 6.5.

[0100] The preparation method of fermentation medium is as follows:

[0101] 1. Add an appropriate amount of drinking water to the batching tank, then put in the raw materials (except maltose) of the formula amount, stir evenly, and pump to 50M 3 In the fermenter, the volume was adjusted to a certain volume with drinking water, and sterilized at 121°C for 30 minutes.

[0102]2. Add an appropriate amount of drinking water to the batching tank, then put in the formula amount of maltose, stir evenly, pump it into the sugar ta...

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Abstract

The invention provides a method for producing lovastatin by fermentation and a fermentation medium used by the same. The fermentation medium takes maltose as a carbon source and does not contain glucose. The method for producing lovastatin by fermentation by using the fermentation medium is characterized in that: Aspergillus terreus for producing lovastatin is cultured by fermentation in the fermentation medium provided by the invention. The method provided by the invention improves the fermentation unit of lovastatin by about 10%, simultaneously shortens the fermentation period, lowers the production cost, and is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to a fermentation medium for producing lovastatin by fermentation and a method for producing lovastatin by fermentation using the fermentation medium. Background technique [0002] Lovastatin is a cholesterol-lowering drug derived from microbial hydroxymethylglutaryl-CoA reductase inhibitors, and its chemical structure is shown in the following formula I. Because of its clear mechanism of action and remarkable clinical efficacy, it is currently an important drug for preventing and treating cardiovascular and cerebrovascular diseases. [0003] [0004] Formula I [0005] Lovastatin is a secondary metabolite of fungi, and the main industrial fungi producing it are Aspergillus terreus and Monascus ruber. [0006] In the prior art, the carbon source of the culture medium for the fermentative production of lovastatin is mainly glucose, or a mixed carbon source of glucose and dextrin,...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12R1/66
Inventor 赵德张葵李宏崔健明向中南
Owner PEKING UNIV FOUNDER GRP CO LTD
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