Genistein hydrogel compound and its preparation method

A technology of genistein and hydrogel, which is applied in the field of genistein composites and its preparation, can solve the problems of inhibition of solubilization effect, insoluble in water, limited application of genistein, etc., and achieve water solubility improvement, The effect of improving water solubility

Inactive Publication Date: 2012-06-13
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Although genistein has many physiological functions, it is insoluble in water, and its application in the food and pharmaceutical industries is limited
Cyclodextrin can be used as a solubilizer, but the solubility of cyclodextrin itself is not high, and the solubilization effect is inhibited
Hydrophilic colloids have good solubility in water and have many physiological effects. However, there are few studies on whether hydrocolloids can solve the problem of limited application of genistein

Method used

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  • Genistein hydrogel compound and its preparation method
  • Genistein hydrogel compound and its preparation method
  • Genistein hydrogel compound and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Weigh 0.2 g of κ-carrageenan and dissolve in 40 mL of distilled water at 80 o C under stirring for 60 min, during the stirring process, the temperature was gradually cooled to 52.12 oC, get kappa-carrageenan gum solution. Weigh 13.5 mg genistein and dissolve in 10 mL, 0.1 mol / L NaOH solution. Add the alkaline solution of genistein to the κ-carrageenan gum solution, so that the concentration of genistein in 50 ml of the mixed solution is 0.27 mg / mL, and stir for 20 s, and then use 5% mass fraction of Adjust the pH to 4.76 with phosphoric acid to obtain an acidic mixture at 50.12 o Stir for 60 min at C, then centrifuge for 25 min at a centrifugal force of 14 000 g, then discard the supernatant, wash the precipitate twice with 50% (v / v) ethanol solution, and collect the composite after lyophilization. things.

[0083] result: E The measured value of c was 50.48 ± 2.35 mg / 100mg.

Embodiment 2

[0085] Weigh 0.2 g of κ-carrageenan and dissolve in 40 mL of distilled water at 80 o C under stirring for 60 min, gradually cooling the temperature to 70 o C, κ-carrageenan gum solution. Weigh 10 mg of genistein and dissolve in 10 mL of 0.1 mol / L NaOH solution. Add the alkaline solution of genistein to the carrageenan solution so that the concentration of genistein in the 50 ml mixed solution is 0.20 mg / mL, stir for 20 s, and then adjust with phosphoric acid with a mass fraction of 5%. When the pH reaches 5, an acidic mixture is obtained at 50.12 o Stir for 60 min at C, then centrifuge for 25 min at a centrifugal force of 14 000 g, then discard the supernatant, wash the precipitate twice with 50% (v / v) ethanol solution, and collect the composite after lyophilization. thing.

[0086] result: E The measured value of c was 3.45 ± 0.12 mg / 100mg.

[0087]

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Abstract

The invention relates to a genistein compound and its preparation method, the genistein hydrogel compound is composed of genistein and hydrogel. The preparation method comprises the following steps: taking hydrogel and adding in an aqueous medium, heating and stirring to fully dissolve to obtain a hydrogel solution; taking genistein and adding in an alkaline aqueous medium to obtain a genistein solution; mixing the obtained hydrogel solution and the obtained genistein solution and stirring, fully drying to obtain the genistein hydrogel compound. According to the invention, composition of a hydrophilic colloid carragheenan and genistein is used for raising the water-solubility of genistein. A single factor experiment is used for respectively inspecting the influence of composite temperature, the composite time, genistein concentration and endpoint pH on composite efficiency. A response surface methodology is used for optimizing the compound preparation process, the obtained technology is capable of producing the compound with the highest composite efficiency, and the water-solubility of genistein in the genistein hydrogel compound can be effectively enhanced.

Description

technical field [0001] The invention relates to the field of food extraction technology, in particular to a genistein compound and a preparation method thereof. Background technique [0002] Genistein, also known as genistein (5,7,4'-trihydroxyisoflavone), is a naturally occurring isoflavone and phenolic compound, widely found in legumes such as soybean, kudzu root, and sophora. Genistein has a variety of biological activities and pharmacological effects, can reduce the risk of cardiovascular and cerebrovascular diseases, reduce the incidence of breast cancer, rectal cancer and other cancers, increase bone density, and prevent osteoporosis, and its structure is similar to estradiol, It can bind to estrogen receptors and sex hormone proteins with low affinity, competitively bind to estradiol receptors, and exert estrogen and anti-estrogen effects, so it is also called phytoestrogens. [0003] Although genistein has many physiological functions, it is insoluble in water, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A61K31/352A61K47/36A61K47/38A61P9/00A61P35/00A61P19/10A61P5/30A61P5/32A23L33/00
Inventor 赵国华彭见林
Owner SOUTHWEST UNIVERSITY
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