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Extracting and separating method for various active ingredients of coix seeds

A technology of active ingredients and separation methods, which is applied in the field of extraction and separation of various active ingredients of coix seed, can solve the problems of destroying the active structure of coix seed polysaccharides, waste of resources, etc., and achieve the effects of avoiding solvent residue, low material consumption, and low energy consumption

Active Publication Date: 2015-11-11
仙芝科技(福建)股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, one kind of utilization of coix seed is direct consumption after rough processing, including pulverization, puffing (patent CN201410580960. The production method of coix seed), puffing and high temperature and high pressure will destroy the active structure of coix seed polysaccharide; in addition, the starch which accounts for half of the weight of coix seed will generate a lot of glucose in the process of human digestion, so it is not suitable for special groups such as overweight, obesity and diabetes.
[0005] The other is to extract only the single active ingredient of coix seed, for example: 1) dietary fiber (patent CN201410580563.2 production method of coix seed dietary fiber and coix seed dietary fiber), 2) protein (patent CN201410580846.7 coix seed protein production method and coix seed protein), 3) polysaccharide (patent CN201210512343.7 a preparation method and application of coix seed polysaccharide and document 1007-8924 (2007) 05-0078-04 ultrafiltration membrane method to concentrate coix seed polysaccharide extract) and 4) Sugars (patent CN201410580830.6 production method of coix seed sugar and CN200910264178.6 production method of coix seed enzymatic hydrolyzate), etc., each of the above methods only obtains one component, and the rest of the components are discarded as waste, resulting in resources great waste of

Method used

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  • Extracting and separating method for various active ingredients of coix seeds

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. 10kg of coix seed is crushed and sieved to 60 mesh, added with 5kg of purified water for wet granulation, sieved to 20 mesh and then dried. 2 Extraction technology for extraction: the pressure of the extraction kettle is 25Mpa, the temperature is 40°C, the pressure of the separation kettle is 8Mpa, the temperature is 45°C, the flow rate is 110L / h, and the extraction time is 4h, 8.80kg of defatted coicis granules and 46g of coixene ester (yield 0.46%, purity 90.10%) and coix seed oil 0.502kg (yield 5.02%);

[0037] 2. Add 8.80 kg of defatted coix seed granules to 100 kg of purified water, 5 g of high-temperature amylase, extract at 95°C for 1 hour, and filter to obtain 105 kg of water extract and coix seed protein. The weight of coix seed protein after drying is 2.25 kg (yield 22.5%, purity 55.83%);

[0038] 3. Use hydrochloric acid to adjust the pH of 105kg of water extract to 4.0, add 5g of glucoamylase, enzymolyze at 65°C for 1 hour, and filter to obtain 105kg of ...

Embodiment 2

[0042] 1. 10kg of coix seed is crushed and sieved to 100 mesh, added with 4kg of purified water for wet granulation, sieved to 20 mesh and dried, and supercritical CO 2 Extraction technology for extraction: the pressure of the extraction kettle is 25Mpa, the temperature is 45°C, the pressure of the separation kettle is 8Mpa, the temperature is 40°C, the flow rate is 120L / h, and the extraction time is 3h, 8.85kg of defatted coicis granules and 45g of coixene ester (yield 0.45%, Purity 90.12%) [3] and coix seed oil 0.471kg (yield 4.71%);

[0043] 2. Add 8.85 kg of defatted coix seed granules to 100 kg of purified water, 5 g of high-temperature amylase, extract at 90°C for 2 hours, filter to obtain 106 kg of water extract and coix seed protein, and the weight of coix seed protein after drying is 2.15 kg (yield 21.5%, purity 57.70%) [4] ;

[0044] 3. Use hydrochloric acid to adjust the pH of 106 kg of water extract to 4.2, add 10 g of glucoamylase, enzymatically hydrolyze at 65...

Embodiment 3

[0048] 1. 10kg of coix seed is crushed and sieved to 80 mesh, added with 6kg of purified water for wet granulation, sieved to 10 mesh and then dried. 2 Extraction technology for extraction: the pressure of the extraction kettle is 25Mpa, the temperature is 40°C, the pressure of the separation kettle is 8Mpa, the temperature is 40°C, the flow rate is 120L / h, and the extraction time is 4h, 8.87kg of defatted coicis granules and 54g of coixene ester (yield 0.54%, Purity 89.97%) [3] and coix seed oil 0.520kg (yield 5.20%);

[0049] 2. Add 8.87kg of defatted coix seed granules to 100kg of purified water, 10g of high-temperature amylase, extract at 95°C for 2 hours, filter to obtain 105kg of water extract and coix seed protein, and the weight of coix seed protein after drying is 2.36kg (yield: 23.6%, purity: 52.94%) ) [4] ;

[0050] 3. Use hydrochloric acid to adjust the pH of 105 kg of water extract to 4.1, add 5 g of glucoamylase, enzymolyze at 60 ° C for 2 hours, and filter t...

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Abstract

The invention discloses an extracting and separating method for various active ingredients of coix seeds. The extracting and separating method includes the steps that after the coix seeds are smashed, prilled and dried, coix seed ester, coix seed oil and degreased coix seed particles are extracted with the supercritical CO2 extraction technology, wherein the yield of the coix seed ester is 0.54%, and the purity of the coix seed ester is 89.97%; assisted aqueous extracting is carried out on the degreased coix seed particles through thermostable amylase, coix seed starch is hydrolyzed, and water extract and coix seed proteins are obtained at the same time, wherein the yield of the coix seed proteins is 21.5%, and the purity of the coix seed proteins is 57.70%; enzymolysis is carried out on the water extract through saccharifying enzymes, and enzymatic hydrolysate is obtained; the enzymatic hydrolysate is separated with the membrane separation technology, and retention fluid and filtered fluid are obtained; the retention fluid is dried, and coix seed extract is obtained, wherein the yield of the coix seed extract is 0.92%, the yield of polysaccharide in the coix seed extract is 0.53%, and the purity of the polysaccharide is 57.61%. The obtained coix seed ester, the obtained coix seed oil, the obtained coix seed proteins and the obtained coix seed extract can be directly used as raw materials of common food, health food or special medical application formula food, and the coix seeds are comprehensively used.

Description

technical field [0001] The invention belongs to the field of food industry processing and natural product separation, and specifically relates to a method for extracting and separating various active components of Coix seed. Background technique [0002] Coix seed referred to in the present invention is grass coix (coix seed) Coixlacryma-jobi L.var.meyuan (Romen.)Stapf ) dry mature seed kernels. [0003] Coix seed contains starch (45-48%), protein (14-16%), lipid (2-6%), active polysaccharide (trace amount) and crude fiber and other substances: coixenyl in coix seed lipid contains antioxidant Tumor, immune regulation and other pharmacological components; coix seed protein contains 8 essential amino acids needed by the human body, which is a high-quality protein; coix seed also contains neutral glucan 1-7 with anti-complement activity and acidic polysaccharide CA -1, CA-2, and coixanA / B / C, which has hypoglycemic effect. [0004] At present, one kind of utilization of coix ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C08H1/00C07C69/58C07C67/58C11B1/10
CPCY02P20/54
Inventor 李晔周岩飞朱忠敏姚渭溪
Owner 仙芝科技(福建)股份有限公司
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