Extracting and separating method for various active ingredients of coix seeds

Abstract

The invention discloses an extracting and separating method for various active ingredients of coix seeds. The extracting and separating method includes the steps that after the coix seeds are smashed, prilled and dried, coix seed ester, coix seed oil and degreased coix seed particles are extracted with the supercritical CO2 extraction technology, wherein the yield of the coix seed ester is 0.54%, and the purity of the coix seed ester is 89.97%; assisted aqueous extracting is carried out on the degreased coix seed particles through thermostable amylase, coix seed starch is hydrolyzed, and water extract and coix seed proteins are obtained at the same time, wherein the yield of the coix seed proteins is 21.5%, and the purity of the coix seed proteins is 57.70%; enzymolysis is carried out on the water extract through saccharifying enzymes, and enzymatic hydrolysate is obtained; the enzymatic hydrolysate is separated with the membrane separation technology, and retention fluid and filtered fluid are obtained; the retention fluid is dried, and coix seed extract is obtained, wherein the yield of the coix seed extract is 0.92%, the yield of polysaccharide in the coix seed extract is 0.53%, and the purity of the polysaccharide is 57.61%. The obtained coix seed ester, the obtained coix seed oil, the obtained coix seed proteins and the obtained coix seed extract can be directly used as raw materials of common food, health food or special medical application formula food, and the coix seeds are comprehensively used.

Description

technical field [0001] The invention belongs to the field of food industry processing and natural product separation, and specifically relates to a method for extracting and separating various active components of Coix seed. Background technique [0002] Coix seed referred to in the present invention is grass coix (coix seed) Coixlacryma-jobi L.var.meyuan (Romen.)Stapf ) dry mature seed kernels. [0003] Coix seed contains starch (45-48%), protein (14-16%), lipid (2-6%), active polysaccharide (trace amount) and crude fiber and other substances: coixenyl in coix seed lipid contains antioxidant Tumor, immune regulation and other pharmacological components; coix seed protein contains 8 essential amino acids needed by the human body, which is a high-quality protein; coix seed also contains neutral glucan 1-7 with anti-complement activity and acidic polysaccharide CA -1, CA-2, and coixanA / B / C, which has hypoglycemic effect. [0004] At present, one kind of utilization of coix ...

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Problems solved by technology

[0004] At present, one kind of utilization of coix seed is direct consumption after rough processing, including pulverization, puffing (patent CN201410580960. The production method of coix seed), puffing and high temperature and high pressure will destroy the active structure of coix seed polysaccharide; in addition, the starch which accounts for half of the weight of coix seed will generate a lot of glucose in the process of human digestion, so it is not suitable for special groups such as overweight, obesity and diabetes.

[0005] The other is to extract only the single active ingredient of coix seed, for example: 1) dietary fiber (patent CN201410580563.2 production method of coix seed dietary fiber and coix seed dietary fiber), 2) protein (patent CN201410580846.7 coix seed protein production method and coix seed protein), 3) polysaccharide (patent CN201210512343.7 a preparation method and application of coix seed polysaccharide and document 1007-8924 (2007) 05-0078-04 ultrafiltration membrane method to concentrate coix seed polysaccharide extract) and 4) Sugars (patent CN201410580830.6 production method of coix seed sugar and CN200910264178.6 production method of coix seed enzymatic hydrolyzate), etc., each of the above methods only obtains one component, and the rest of the components are discarded as waste, resulting in resources great waste of

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