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Application of GmABCA9 gene in improving soybean protein content and grain weight

A protein content and protein technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2019-07-23
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the increase in soybean production in my country cannot meet people's needs for soybean protein, so increasing soybean production while increasing protein content is an important direction for soybean breeding

Method used

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  • Application of GmABCA9 gene in improving soybean protein content and grain weight
  • Application of GmABCA9 gene in improving soybean protein content and grain weight
  • Application of GmABCA9 gene in improving soybean protein content and grain weight

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Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1, GmABCA9 gene improves soybean protein content and grain weight

[0058] The nucleotide sequence of the GmABCA9 gene involved in this example is shown in SEQ ID No.2, and the amino acid sequence of its encoded protein is shown in SEQ ID No.1.

[0059] 1. Construction of overexpression vector

[0060] 1. Intermediate carrier construction

[0061] Using soybean Tianlong No. 1 leaf cDNA as a template, gene cloning primers (CDS-fw and CDS-rw) were amplified with the PrimerStar Max (Takara, Japan) kit, and the reaction solution (50 μl) was prepared as shown in Table 1.

[0062] Table 1 reaction system

[0063] Reagent Usage amount Final concentration PrimerSTAR Max Premix(2×) 25μl 1× CDS-fw 10pmol 0.2μM CDS-rw 10pmol 0.2μM template 200ng Sterile distilled water Up to 50μl

[0064] Denaturation at 98°C for 10 sec, annealing at 58°C for 15 sec, extension at 72°C for 3 min, a total of 30 cycles, ampli...

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Abstract

The invention discloses an application of a GmABCA9 gene in improving soybean protein content and grain weight. The invention provides an application of GmABCA9 protein or a related biomaterial thereof in regulating protein content and / or yield of plant seeds. The related biomaterial is a nucleic acid molecule expressing the GmABCA9 protein or an expression cassette, a recombinant vector, recombinant bacteria or a transgenic cell line containing the nucleic acid molecule. Experiments prove that after the GmABCA9 gene is transferred to soybeans, the protein content and hundred-grain weight of seeds of transgenic plants over-expressed by the gene are significantly higher than those of non-transgenic control plants, which indicates that the GmABCA9 and a coding gene thereof can regulate protein content and grain weight of plant seeds. The GmABCA9 and related biomaterial thereof can be used for improving the soybean quality and increasing the soybean yield. The GmABCA9 has the potential ofbeing applied to breeding and creation of new varieties of soybeans.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of GmABCA9 gene in increasing soybean protein content and grain weight. Background technique [0002] Soybean is one of the important food crops and oil crops, and one of the main sources of vegetable protein for human diet and livestock breeding. Soy protein is a plant-based complete protein. In addition to having the same nutritional value as animal protein, it also has the incomparable advantages of animal protein, that is, it does not contain cholesterol and contains isoflavones with cholesterol-lowering effects. Soybean protein content is one of the important target traits in soybean breeding. At present, the increase of soybean yield in my country cannot meet people's demand for soybean protein, so increasing soybean yield while increasing protein content is an important direction of soybean breeding. The 100-seed weight is an important factor affecting soybea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/10A01H6/54
CPCC07K14/415C12N15/8251C12N15/8261
Inventor 郭葳单志慧张婵娟陈李淼张晓娟袁松丽郝青南邱徳珍黄毅曹东陈水莲油清波代文君包爱丽杨中路陈海峰周新安
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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