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Method for extracting plukenetia volubilis seed protein

A technology of star oil vine and protein, which is applied in the field of protein extraction from star oil vine seeds, and can solve the problems of little research on protein

Inactive Publication Date: 2014-07-02
XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the domestic and foreign researches on star oil rattan are mainly the development and utilization of oil. Star oil rattan oil has the functions of nourishing skin, delaying aging, regulating blood lipids, etc.; but there is little research on protein in seed kernels

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Crush the star oil vine cake, pass through a 100-mesh sieve, degrease with n-hexane for 4 h, and place it in a fume hood for 12 h, and evaporate the solvent to obtain a degreasing powder.

[0012] Weigh 5 g of defatted powder, add 150 ml of sodium hydroxide solution (pH 12), stir magnetically at 50 °C for 120 min, then centrifuge at 4000 r / min for 20 min, repeat the extraction once for the precipitate, and combine the two extractions supernatant. The pH of the above supernatant was adjusted to 3.5 with dilute acid, settled at 4 °C for 10 h, the separated precipitate was filtered and washed three times with distilled water, and finally dried in a freeze dryer to obtain 2.478 g of protein, with an extraction rate of 76.47%.

Embodiment 2

[0014] Crush the star oil vine cake, pass through a 80-mesh sieve, degrease with n-hexane in a fat extractor for 4 hours, and then place it in a fume hood for 12 hours, and evaporate the solvent to obtain a degreased powder.

[0015] Weigh 5 g of defatted powder, add 150 ml of sodium hydroxide solution (pH 9), stir magnetically at 50 °C for 120 min, then centrifuge at 4000 r / min for 20 min, repeat the extraction once for the precipitate, and combine the two extractions supernatant. The pH of the above supernatant was adjusted to 5 with dilute hydrochloric acid, settled at 4 °C for 10 h, the precipitate separated by filtration was washed with distilled water three times, and finally dried in a freeze dryer to obtain 1.812 g of protein, with an extraction rate of 55.92%.

Embodiment 3

[0017] Crush the star oil vine cake, pass through a 60-mesh sieve, degrease with n-hexane in a fat extractor for 4 hours, then place it in a fume hood for 12 hours, and evaporate the solvent to obtain a degreased powder.

[0018] Weigh 5 g of defatted powder, add 150 ml of sodium hydroxide solution (pH 12), stir magnetically at 60 °C for 120 min, then centrifuge at 4000 r / min for 20 min, repeat the extraction once for the precipitate, and combine the two extractions supernatant. The pH of the above supernatant was adjusted to 4 with dilute hydrochloric acid, settled at 4 °C for 10 h, the separated precipitate was filtered and washed with distilled water three times, and finally dried in a freeze dryer to obtain 2.201 g of protein, with an extraction rate of 67.92%.

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Abstract

The invention discloses a method for extracting plukenetia volubilis seed protein. The method comprises the following steps: taking squeezed (extracted) plukenetia volubilis cake as a raw material; screening after drying and crushing; extracting the protein by adopting an alkali-solution and acid-isolation method after degreasing, wherein the technological parameters of alkali-solution and acid-isolation extraction are as follows: the ratio of solid to liquid, namely the mass-to-volume ratio (w / v) of degreased powder to dilute alkali lye is (1:1) to (1:100); the pH of the dilute alkali lye is 7.0-13.0; the extraction temperature is 20-80 DEG C, and the extraction time is 30-3000 minutes; separating out protein sediments after acid isolation, and drying to form protein powder. The method is simple in process, low in cost, and high in protein yield, and guidance is provided for research and development of related products of the plukenetia volubilis seed protein.

Description

technical field [0001] The invention belongs to the field of food processing, and in particular relates to a method for extracting star oil rattan seed protein. Background technique [0002] Star oil vine ( Plukenetia volubilis Linneo), also known as Inca fruit, Inca peanut, American vine fruit, South American oil vine, or Sacha Inchi in Inca, is a perennial woody vine of Euphorbiaceae (Euphorbiaceae), originally growing in the Andes Mountains of South America at an altitude of 80-1700 m area. Introduced to China in 2006, it was planted in Yunnan Province, such as the Xishuangbanna Tropical Botanical Garden of the Chinese Academy of Sciences, and Guangdong Province, such as the South China Botanical Garden of the Chinese Academy of Sciences. The trial planting was successful in 2008. In addition to being rich in polyunsaturated fatty acids, star oil rattan seed kernels are also rich in protein, with a protein content of 25-30%. The protein contains a variety of amino acid...

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K1/30
Inventor 赵旻许又凯
Owner XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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