Preparation method for camellia seed polypeptide

A technology of camellia oleifera seeds and camellia oleifera seed meal, which is applied in the field of preparation of camellia oleifera seed polypeptides, and achieves the effects of improving utilization rate, good extraction effect and mild extraction process conditions.

Active Publication Date: 2014-03-12
衢州刘家香食品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no domestic and foreign research reports on the polypeptides of camellia oleifera. Therefore, it is of great practical significance to carry out research on the health function and activity of polypeptides of camellia oleifera

Method used

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  • Preparation method for camellia seed polypeptide
  • Preparation method for camellia seed polypeptide
  • Preparation method for camellia seed polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of Camellia oleifera polypeptide, the preparation method is specifically as follows:

[0025] (1) Enzymolysis: Mix the pulverized camellia seed meal powder with 80 mesh sieve with water according to the ratio of material to liquid 1:6, adjust the pH value to 7.25, add 0.04% compound plant hydrolytic enzyme of camellia seed meal powder, Enzyme hydrolyze at 65°C for 3.5h, inactivate the enzyme at 85°C for 10min after enzymolysis, and then centrifuge at 3000rpm for 15min to obtain the residue of Camellia oleifera seed meal;

[0026] (2) Ultrasonic-assisted alkaline extraction: mix the Camellia oleifera seed meal residue (on a dry basis) obtained in step (1) with water at a mass ratio of 1:25, adjust the pH value to 10.0, and conduct alkaline extraction at 40°C for 90 minutes. Ultrasound is used to assist in the extraction process. The power of the ultrasonic wave is 300W, and the ultrasonic time is 20min.

[0027] (3) Acid precipitation: adjust the pH...

Embodiment 2

[0030] A preparation method of Camellia oleifera polypeptide, the preparation method is specifically as follows:

[0031] (1) Enzymolysis: Mix the pulverized Camellia oleifera seed powder through a 100-mesh sieve with water at a material-to-liquid ratio of 1:7, adjust the pH value to 7.30, add 0.05% compound plant hydrolytic enzyme of Camellia oleifera seed powder, Enzymolysis at 70°C for 3 hours, after enzymolysis, inactivate the enzyme at 85°C for 15 minutes, then centrifuge at 3200rpm for 10 minutes to obtain the residue of Camellia oleifera seed meal;

[0032] (2) Ultrasonic-assisted alkaline extraction: mix the Camellia oleifera seed meal residue (on a dry basis) obtained in step (1) with water at a mass ratio of 1:25, adjust the pH value to 10.5, and carry out alkaline extraction at 45°C for 80 minutes. The extraction process is assisted by ultrasonic waves, the power of the ultrasonic wave is 1000W, and the ultrasonic time is 20min. After the alkali extraction is comple...

Embodiment 3

[0036] A preparation method of Camellia oleifera polypeptide, the preparation method is specifically as follows:

[0037] (1) Enzymolysis: Mix the pulverized camellia seed meal powder with 100-mesh sieve with water at a material-to-liquid ratio of 1:6, adjust the pH value to 7.28, add compound plant hydrolytic enzymes with 0.048% of the camellia seed meal powder mass, Enzymolyze at 68°C for 3.25h, inactivate the enzyme at 90°C for 5min after enzymolysis, and then centrifuge at 3000rpm for 20min to obtain the residue of Camellia oleifera seed meal;

[0038] (2) Ultrasonic-assisted alkaline extraction: mix the Camellia oleifera seed meal residue (on a dry basis) obtained in step (1) with water at a mass ratio of 1:25, adjust the pH value to 10.0, and carry out alkaline extraction at 40°C for 80 minutes. Ultrasound is used to assist in the extraction process, the power of the ultrasonic wave is 800W, and the ultrasonic time is 20min. After the alkali extraction is completed, the ...

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Abstract

The invention relates to a preparation method for camellia seed polypeptide. The method comprises the following steps: crushing camellia seed protein, sieving by a 80-100 mesh sieve, and adding water according to the mass-to-volume ratio of 3:(100-120) to prepare a suspending liquid; enabling the suspending liquid to be subjected to heat treatment for 10-20 min at the temperature of 90-95 DEG C, adjusting the pH value to obtain a neutral substance after cooling; adding neutral protease according to the principle that each gram of substrate contains 4300-4400 u of enzyme, then performing enzymolysis for 3.5-4.5 h at the temperature of 53-54 DEG C; performing enzyme deactivation after the termination of the enzymolysis, and obtaining a supernatant fluid through centrifugation; allowing the supernatant fluid to be subjected to desalination and column chromatography, then allowing an eluant to be subjected re-centrifugation to obtain another supernatant fluid, and freeze-drying the supernatant fluid to obtain the camellia seed polypeptide. According to the method, the extraction process condition is mild, the prepared camellia seed meal protein is excellent in shape and color; the camellia seed protein is subjected to enzymolysis of the neutral protease, desalination and column chromatography, then the eluant subjected to the column chromatography is freeze-dried so as to obtain the camellia seed polypeptide. The camellia seed polypeptide is low in impurity content and rich in amino acid content, and can be widely applied to food additives.

Description

technical field [0001] The invention relates to a preparation method of a polypeptide, in particular to a preparation method of a camellia oleifera seed polypeptide. Background technique [0002] Camellia oleifera belongs to the small evergreen trees, is the genus Camellia of the family Theaceae, and is an excellent woody oil plant in southern my country. Camellia oleifera resources in my country are very rich, and the planting area of ​​camellia oleifera forest alone reaches 4 million hectares 2 , is the country with the most varieties of Camellia oleifera, the widest distribution and the highest yield of Camellia oleifera seeds in the world, with an annual output of about 549,800 tons of Camellia oleifera seeds, 150,000 tons of camellia oil, and an average annual output of 397,100 tons of Camellia oleifera seed meal, a by-product of Camellia oleifera seeds Ton. The protein content in Camellia oleifera seed meal is about 12% to 15%, and its amino acid composition is 17 ki...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/30
Inventor 何东平胡传荣双杨刘京伟
Owner 衢州刘家香食品有限公司
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