The present invention discloses a preparation method and a detection method of phospholipid tea polyphenol dried fish floss. The preparation method comprises the following steps: firstly, washing fish; secondly cooking the fish; thirdly, cooling the fish and removing fish skin, fat and fish bones; fourthly, deodorizing; fifthly, squeezing; sixthly, adding a phospholipid tea polyphenol compound; seventhly, rubbing the floss and continuously adding edible salt, white granulated sugar and pea powder; eighthly, stir-frying the floss using low heat until a water content is about 12%; and ninthly, stir-frying the floss to be crisp, and conducting cooling, canning and storing. The prepared phospholipid polyphenol dried fish floss by the preparation method can release tea polyphenols slowly, thusa long-term anti-oxidation effect is played, and a storage period of the dried crucian floss is improved. Besides, the detection method can quickly and accurately detect a fatty acid content in the dried fish floss and further determines an oxidation situation of meat quality of the dried fish floss.