Stable controllable high-quality Pu'er tea and fermentation production method thereof
A production method and technology for Pu'er tea, applied in the field of high-quality Pu'er tea and its fermentation production, can solve the problems of lack of control of tea pile temperature, humidity, etc., inability to guide Pu'er tea parameters, and inability to control air flow, etc., and achieve complete fermentation process. Controllable, uniform and controllable fermentation process, and the effect of eliminating unknown microbial contamination
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Embodiment 1
[0030] In this embodiment, a stable and controllable high-quality Pu'er tea and its fermentation production method comprise the following steps:
[0031] (1) Strain preparation:
[0032] The microorganisms used for the fermentation of Pu’er tea that can be used in food production refer to Aspergillus niger (Aspergillus niger3.350), Aspergillus oryzae (Aspergillus oryzae AS3.042), Bacillus subtilis (Bacillus subtilis1389), Saccharomyces cerevisiae, Candida rubenosa and lactic acid bacteria, A small amount of preserved microorganisms can be picked under aseptic conditions and cultured on the corresponding agar medium. Among them, Aspergillus niger, Aspergillus oryzae, Saccharomyces cerevisiae, and Candida ruberens can be inserted into PDA solid medium and cultured at 28°C Cultivate under certain conditions; Bacillus subtilis can be inserted into LB solid medium and cultured at 37°C; lactic acid bacteria can be inserted into MRS solid medium and cultured at 37°C. After culturing...
Embodiment 2
[0053] In this embodiment, a stable and controllable high-quality Pu'er tea and its fermentation production method comprise the following steps:
[0054] (1) Strain preparation:
[0055] The microorganisms used for the fermentation of Pu’er tea that can be used in food production refer to Aspergillus niger (Aspergillus niger3.350), Aspergillus oryzae (Aspergillus oryzae AS3.042), Bacillus subtilis (Bacillus subtilis1389), Saccharomyces cerevisiae, Candida rubenosa and lactic acid bacteria, A small amount of preserved microorganisms can be picked under aseptic conditions and cultured on the corresponding agar medium. Among them, Aspergillus niger, Aspergillus oryzae, Saccharomyces cerevisiae, and Candida ruberens can be inserted into PDA solid medium and cultured at 28°C Cultivate under certain conditions; Bacillus subtilis can be inserted into LB solid medium and cultured at 37°C; lactic acid bacteria can be inserted into MRS solid medium and cultured at 37°C. After culturing...
Embodiment 3
[0073] In this embodiment, a stable and controllable high-quality Pu'er tea and its fermentation production method comprise the following steps:
[0074] (1) Strain preparation:
[0075] The microorganisms used for the fermentation of Pu’er tea that can be used in food production refer to Aspergillus niger (Aspergillus niger3.350), Aspergillus oryzae (Aspergillus oryzae AS3.042), Bacillus subtilis (Bacillus subtilis1389), Saccharomyces cerevisiae, Candida rubenosa and lactic acid bacteria, A small amount of preserved microorganisms can be picked under aseptic conditions and cultured on the corresponding agar medium. Among them, Aspergillus niger, Aspergillus oryzae, Saccharomyces cerevisiae, and Candida ruberens can be inserted into PDA solid medium and cultured at 28°C Cultivate under certain conditions; Bacillus subtilis can be inserted into LB solid medium and cultured at 37°C; lactic acid bacteria can be inserted into MRS solid medium and cultured at 37°C. After culturing...
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