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Method for rapidly identifying theabrownin

A theabrownin and rapid technology, applied in the field of chemical identification, can solve the problems of large difference in results, expensive machines, slow speed, etc., and achieve the effect of small detection workload, short detection time, and small batch-to-batch difference

Inactive Publication Date: 2015-05-13
杭州茗褐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Now there is a new method for the detection of theabrownin by high performance liquid chromatography or mass spectrometry or liquid-mass chromatography. The obtained data is accurate, but it also has some defects, such as expensive machines, slow speeds, and limited operator skills. High requirements, high performance liquid chromatography is too precise, and the measured results vary greatly

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  • Method for rapidly identifying theabrownin
  • Method for rapidly identifying theabrownin
  • Method for rapidly identifying theabrownin

Examples

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Embodiment 1

[0028] A method for quickly identifying theabrownin of the present invention comprises the following steps:

[0029] 1) Qualitative detection: After preparing a theabrownin solution with a theabrownin concentration of 1g / L, take 3 μL of theabrownin solution and detect the absorbance of theabrownin at a wavelength of 200-850nm on a k5600 ultra-micro ultraviolet spectrophotometer. Brownin has a characteristic absorption peak at 273nm±2nm, and the UV-VIS full-wavelength scanning peak type is shown in figure 1 ;

[0030] 2) Quantitative detection: After preparing the theabrownin solution to be tested with a concentration of 4g / L, take 2ml of theabrownin solution and detect that the absorption peak of theabrownin is 496062uV.S on a capillary electrophoresis instrument, wherein the electrophoresis condition is: buffer solution It is borax, the capillary length is 50cm, the electrophoresis time is 900s, the injection pressure is 1kpa, the flushing pressure is 40kpa, and the detectio...

Embodiment 2

[0034] A method for quickly identifying theabrownin of the present invention, the method is:

[0035] After preparing a theabrownin solution with a theabrownin concentration of 2 g / L, take 4 μL of theabrownin solution to detect the absorbance of theabrownin at a wavelength of 200-850 nm on a k5600 ultra-micro ultraviolet spectrophotometer, and the absorbance of theabrownin at 273nm ± A characteristic absorption peak appears at 2nm, and the UV-VIS full-wavelength scanning peak type is shown in image 3 ;

[0036] The beneficial effects of a method for quickly identifying theabrownin in this embodiment include: accurate detection results, short detection time; little influence by human factors, small inter-batch difference in determination; small detection workload; standardized detection steps.

Embodiment 3

[0038] A method for quickly identifying theabrownin of the present invention comprises the following steps:

[0039] 1) Qualitative detection: After preparing a theabrownin solution with a theabrownin concentration of 2 g / L, take 2 μL of theabrownin solution and detect the absorbance of theabrownin at a wavelength of 200-850 nm on a k5600 ultra-micro ultraviolet spectrophotometer. Brownin has a characteristic absorption peak at 273nm±2nm, and the UV-VIS full-wavelength scanning peak type is shown in Figure 4 ;

[0040] 2) Quantitative detection: after preparing the theabrownin solution to be tested with a concentration of 6g / L, take 3ml of theabrownin solution and detect the peak height of theabrownin on a capillary electrophoresis instrument as 20679mV, wherein the electrophoresis condition is: the buffer solution is borax , the capillary length is 50cm, the electrophoresis time is 900s, the injection pressure is 1kp, the washing pressure is 40kpa, and the detection wavelen...

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Abstract

The invention discloses a method for rapidly identifying theabrownin. The method comprises the following steps: (1) qualitative detection, namely, after preparing a theabrownin solution, detecting an absorbance of the theabrownin solution by using an ultramicro ultraviolet spectrophotometer to obtain a characteristic peak pattern of the theabrownin; and (2) quantitative detection, namely, after preparing the theabrownin solution, detecting an absorption peak of the theabrownin solution under a specific wavelength of 273nm, and calculating the content of the theabrownin by virtue of the peak area or peak height of the absorption peak. The method for rapidly identifying the theabrownin, disclosed by the invention, has the beneficial effects of accurate detection result, short detection time, little influence of artificial factors, small difference between determined batches, small detection workload, and standardized detecting steps.

Description

technical field [0001] The invention relates to the field of chemical identification, in particular to a method for rapidly identifying theabrownin. Background technique [0002] Existing identification methods of theabrownin (tea pigment) are divided into qualitative and quantitative methods. The traditional UV absorption qualitative method uses an ordinary spectrophotometer, the absorption peak of theabrownin is 273nm ± 20nm, and the traditional colorimetric method based on the standard curve Standards are required for the method. Since theabrownin (tea pigment) is a mixed polymer, there is no real standard, and the operation time is relatively long (more than 1 hour). The standard curve needs to be redone every time. The error of the value determined by this method is relatively large, and the content may even exceed 100%. [0003] Now there is a new method for the detection of theabrownin by high performance liquid chromatography or mass spectrometry or liquid-mass chro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N27/447
Inventor 张进
Owner 杭州茗褐生物科技有限公司
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