Maize kernel size mutant ks and its application

A mutant and grain technology, applied in the field of plant genetic engineering and molecular biology, can solve the problems of grain shrinkage, small grain size, and hollow peel.

Inactive Publication Date: 2017-12-22
MAIZE RES INST SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mu transposon insertion mutation of the ZmSWEET4c gene reported by Sosso et al. led to shrunken grains, hollow pericarp, and reduced fertility, while the ks mutant only showed reduced grain size and normal fertility, indicating that the mutation of the ZmSWEET4c gene and the ZmSWEET4 gene There are differences in cluster mutations

Method used

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  • Maize kernel size mutant ks and its application
  • Maize kernel size mutant ks and its application
  • Maize kernel size mutant ks and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The acquisition of embodiment 1 mutant material

[0039] In 2000, a natural mutant with small kernels was found in the process of conventional breeding in the field, and it was named ks (kernelsmall). After selfing the mutant, it was found that F 1 F 1 F 2 In the second generation, two phenotypes appeared in the grain, one was consistent with that of the normal parent, and the other was significantly smaller than the normal parent, and the segregation ratio was 3:1. Regardless of the reciprocal cross, the results were consistent, and the traits were stable in different locations and years. Under different genetic backgrounds, ks showed smaller grain size, so the mutation of KS gene directly led to smaller grain size. The traits of the mutant have been stably inherited through multiple generations of self-crossing. When observing and comparing the plant morphology of the mutant and the wild type throughout the growth cycle under field conditions, it was found that the...

Embodiment 2

[0040] Embodiment 2 grain phenotype identification

[0041] 1. Grain shape comparison

[0042] Select representative wild-type fruit ears and the mutant ks fruit ears of the present invention, and its appearance contrast sees attached figure 1 shown. Among them, WT on the left is the wild-type ear, and F in the middle 1 The surrogate ear, the ks on the right is the ear of the mutant ks.

[0043] 2. Scanning electron microscope observation of internal structure

[0044] Select representative wild-type grains and mutant ks grains of the present invention. After the grains are dried, tap lightly in the middle of the grains to make them longitudinally fracture in a natural state, remove both ends, and make a cross-section of about 2mm , stick it on the sample stage with double-sided tape, spray gold coating on the ion sputtering instrument, use JSM-6610LV scanning electron microscope, observe the fault structure of the grain under the acceleration voltage of 15kV, and choose t...

Embodiment 3

[0068] Example 3 Obtaining of the ks mutant gene of corn grain volume mutant

[0069] 1. Construction of isolated groups

[0070] Simultaneously with the genetic analysis of the grain size mutation gene, the construction of segregating populations has been carried out, and the F 2 group. For each group, 60 normal grains and 60 small grains were selected respectively, and seedlings germinated. One young leaf was taken from each seedling for DNA preparation. At the same time, the parental leaves were taken to prepare the parental DNA.

[0071] 2. Extraction and purification of total DNA from maize genome and creation of DNA pool

[0072] The DNA was extracted according to the method of McCouch et al. (1998) and improved, and the RNA was removed and quantified with a standard amount of λDNA. In the segregation population, the DNA of 30 normal grains and small grains were mixed in equal amounts to form the gene pool (BulkN, BulkS) for DNA analysis.

[0073] 3. SSR marker ana...

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Abstract

The invention specifically relates to a maize kernel size mutant ks deleted of a maize hexose transport protein gene (ZmSWEET4) and its application. According to a technical scheme in the invention, a coding gene is cloned from the maize mutant ks, and verification results show that the coding gene can regulate and control the maize kernel filling process so as to influence kernel sizes. Experiments of overexpression of the gene cluster in common maize are carried out; and the kernel output of overexpressed transgenic plants is obviously higher than the kernel output of a control group, and transgenic crops with higher output than the output of wild plants can be obtained. The gene obtained in the invention provides a theoretic basis and gene source for cultivation of new crop varieties and plays an important role in genetic improved breeding of germplasm resources of maize.

Description

technical field [0001] The invention belongs to the technical fields of plant genetic engineering and molecular biology, and in particular relates to a corn grain volume mutant ks lacking a corn hexose transporter gene (ZmSWEET4) and an application thereof. Background technique [0002] Maize is an important food crop and a typical C4 model plant in my country, and plays an important role in the research of food production and functional genomics of monocots. With the rapid growth of global corn demand, the status of corn in the national economy has become increasingly prominent. The demand for corn in the world is increasing year by year, and the corn consumption structure has undergone fundamental changes, that is, from the main food crop that solves food and clothing, it has gradually developed into a diversified pattern of livestock feed, industrial raw materials, table non-staple food, and energy crops. Especially in recent years, the fields of renewable energy and dee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N1/21A01H5/00C12R1/01
CPCC07K14/415C12N15/8261
Inventor 于彦丽孟昭东李艳娇赵勐孙琦李文才李文兰庞凯元
Owner MAIZE RES INST SHANDONG ACAD OF AGRI SCI
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