Evaluation method of safety of genetically modified insect resistant rice relative to parasite anagrus nilaparvatae

A technology of rice lice wasps and safety evaluation, which is applied in animal husbandry and other fields

Inactive Publication Date: 2014-07-23
HUAZHONG AGRI UNIV
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Problems solved by technology

[0003] However, while the use of insect-resistant transgenic plants brings benefits to humans, there are also certain potential ecological risks.

Method used

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  • Evaluation method of safety of genetically modified insect resistant rice relative to parasite anagrus nilaparvatae
  • Evaluation method of safety of genetically modified insect resistant rice relative to parasite anagrus nilaparvatae
  • Evaluation method of safety of genetically modified insect resistant rice relative to parasite anagrus nilaparvatae

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Embodiment Construction

[0029] The Bt gene-transformed insect-resistant rice of the present invention selects the cry2Aa gene-transformed rice T2A-1, which expresses the Cry2Aa protein, which is a kind of Bt protein, which has high resistance to Lepidoptera pests, and the non-transgenic parents are selected for lighting Hui 63 rice , and cry2Aa transgenic rice T2A-1 seeds were provided by the State Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University. Minghui 63 rice and cry2Aa transgenic rice T2A-1 were grown to a height of about 15 cm in Yoshida nutrient solution in a cultivation tray of length × width × height = 25 cm × 20 cm × 3 cm, and then used for evaluation experiments. The conditions for cultivating rice seedlings are temperature (26±2°C) and relative humidity (80%).

[0030] Due to the limited content of Cry2Aa protein expressed by transgenic rice, the applicant purchased the Cry2Aa protein produced by Beijing Leshining Technology Co., Ltd. The protein is the same B...

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Abstract

The invention relates to an evaluation method of safety of genetically modified insect resistant rice relative to parasite anagrus nilaparvatae. The evaluation method includes three parts of Tier-1 toxicity determination test, wherein the potential toxicity of Bt proteins expressed by Bt-gene modified insect resistant rice on non-target organism anagrus nilaparvatae is evaluated; successive generation three-level nutrition test, wherein the influence of the Bt-gene modified insect resistant rice on the safety of the anagrus nilaparvatae through parasitic mediators is evaluated according to the food chain of rice-delphacidae-anagrus nilaparvatae; a protein transfer rule, wherein the way and degree that the anagrus nilaparvatae is exposed in the Bt proteins expressed by the Bt-gene modified insect resistant rice are identified through an ELISA detection means; finally, the ecological consequences generated by genetically modified crop cultivation on non-target organisms are evaluated synthetically according to the data of the three parts. The evaluation method is systematic and scientific, and has great significance for the research of the influence of genetically modified crops on the non-target organisms and ecological safety of genetically modified crop cultivation.

Description

technical field [0001] The invention belongs to the technical field of ecological risk assessment of transgenic crops, and in particular relates to a method for evaluating the safety of transgenic insect-resistant rice against the parasitic natural enemy, the rice locust wasp. Background technique [0002] Rice is one of the most important food crops in the world, and more than 3 billion people in the world depend on rice for food. At the same time, rice is one of the most pest-damaged food crops. Every year, due to the damage of rice leaf rollers, borer borers and other Lepidoptera insects, major yield losses are caused (Cheng, 1996; Nathan, 2006). In actual production, the control of lepidopteran pests has long relied on chemical pesticides. The abuse of chemical pesticides not only increases the production cost of rice, but also pollutes the environment. It is also easy to cause pests to develop resistance and reduce the efficacy of pesticides (Su et al. , 2003). Theref...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 华红霞韩宇何月平赵景蔡万伦
Owner HUAZHONG AGRI UNIV
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