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Method for determining N-DAMO (nitrite-dependent anaerobic methane oxidation) rate

An anaerobic methane, oxidation rate technology, applied in the field of environmental research, can solve the problems of inability to accurately and quantitatively evaluate the significance of denitrification combined with anaerobic methane oxidation reaction activity, and achieve the effect of convenient operation and simple equipment

Inactive Publication Date: 2015-07-01
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Traditional research on denitrification combined with anaerobic methane oxidation focuses on the use of molecular biology methods to study its existence in nature and its enrichment application in bioreactors, but it is impossible to accurately and quantitatively evaluate the denitrification combined with anaerobic methane oxidation reaction. activity and its significance in the natural carbon cycle and wastewater treatment

Method used

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  • Method for determining N-DAMO (nitrite-dependent anaerobic methane oxidation) rate
  • Method for determining N-DAMO (nitrite-dependent anaerobic methane oxidation) rate

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specific Embodiment approach

[0012] 1. Mixing and packing samples: Weigh 80g of soil, sediment or activated sludge and 160g of soil pore water, sediment overlying water or activated sludge reactor sewage into a 500ml serum bottle (7), and then put a Magnetic stirrer rotor (10). Serum bottle cap (6) is drilled with two diameters with electric drill and is the hole of 5mm, inserts two lengths in the hole and is respectively 3cm and 2cm the plastic tube that outer diameter is 5mm, and uses all-purpose glue and serum bottle cap (6 ) glue even seal. One end of the 3cm-long plastic tube (4) close to the inside of the serum bottle (7) is connected with a micro-aeration head (9) with a silicone tube (3), and the other end is separated from a high-purity helium cylinder (1) with a silicone tube (3). Pressure valve (2) outlet connection. 2cm long plastic pipe (5) is used as aeration outlet. Cover the serum bottle cap (6), place the serum bottle (7) on the magnetic stirrer (11) (model: 85-2A digital speed measuri...

specific Embodiment

[0018] Selective denitrification combined with anaerobic methane oxidation bacteria abundance value higher than 1.0×10 7 copies·g -1 Dry soil Deep soil samples from a paddy field in Jiangyin, Jiangsu Province were used to measure the rate of denitrification combined with anaerobic methane oxidation. Then specifically implement the introduction in the method, mixing, aliquoting, pre-incubating, incubating and measuring samples. It is calculated that 1.57g of soil is arranged in each glass bottle, and the reaction in the upper space of the experimental group Labco glass sampling bottle (12) is finally obtained at 24h, 48h, 72h, 96h and 120h. 45 CO 2 2.17, 2.35, 2.56, 2.91 and 3.20nmol, respectively, in the negative control group 45 CO 2 % of total CO 2The percentage content is kept at a lower level of 1.10% to 1.20%, experimental data are available. The data of the experimental group was linearly regressed to time, with a slope of 0.281 (nmol CO 2 d -1 ), R 2 = 0.973. ...

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Abstract

The invention discloses a method for determining an N-DAMO (nitrite-dependent anaerobic methane oxidation) rate, belongs to the field of environmental research, and discloses a method for determining an N-DAMO rate of soil, sediments or activated sludge by using an isotope tracer technique. The method disclosed by the invention comprises a sample mixing and subpackaging device, an anaerobic environment creation device and an anaerobic environment creation method, a method for pretreating samples before being determined, and a sample determining and calculating method. Under the conditions of high-purity helium protection, samples are uniformly mixed and subpackaged, and then an anaerobic environment is created by using a high-purity helium to blow the sample mixture. After residual NO2<->, NO3<-> and O2 in a reaction system are consumed by pre-culturing, an electron acceptor and a labeled substrate <13>C-CH4 are added into a reactant, and by measuring the contents of <45>CO2 generated by reacting at different time nodes, an N-DAMO rate is calculated. According to the method, equipment is simple and the operation is easy, and a strict anaerobic environment can be created without being operated in an anaerobic tank. An N-DAMO rate is calculated by labeling a reaction substrate by using an isotope and determining a labeled product, and the calculation of the N-DAMO rate is not affected by other physical and chemical changes.

Description

Technical field: [0001] The invention belongs to the field of environmental research and relates to a method for measuring the denitrification combined anaerobic methane oxidation rate of soil, sediment or activated sludge by using isotope tracer technology. Background technique: [0002] Denitrification combined with anaerobic methane oxidation (nitrite-dependent anaerobic methane oxidation, n-damo) means that under anaerobic conditions, NO 2 - and NO 3 - As an electron acceptor is reduced, methane is oxidized as an electron donor, and finally N 2 and CO 2 the process of. The process has attracted a lot of attention since it was first discovered in a bioreactor in 2006. Natural ecosystems such as paddy fields and wetlands are important sources of methane globally, and denitrification combined with anaerobic methane oxidation can convert CH 4 This powerful greenhouse gas is oxidized to less greenhouse CO 2 , which has important scientific significance in global warmi...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 祝贵兵夏超
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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