Anti-aging composition derived from space breeding desert algae as well as preparation method and application of anti-aging composition

Abstract

The invention discloses an anti-aging composition derived from space breeding desert algae as well as a preparation method and application of the anti-aging composition, and relates to the technical field of application of desert algae. The anti-aging composition is an extract of Hassalliasp.P06 after space breeding, and the preservation number of the Hassalliasp.P06 is CCTCC (China Center for Type Culture Collection) M20242054, and the preservation number of the Hassalliasp.P06 is CCTCC M20242054. The anti-aging composition derived from the space breeding desert algae comprises the following components: polypeptide, free amino acid, polysaccharide and cyclic adenosine monophosphate. The skin care product has very strong moisturizing capacity, can promote the proliferation of skin fibroblasts and remarkably improve the skin compactness, has a soothing effect and oxidation resistance, and can be applied to skin care products or cosmetics to be used as a compactness agent or an anti-aging agent so as to improve the skin aging symptom.

Description

Technical Field

[0001] The present invention relates to the technical field of desert algae applications, and in particular to an anti-aging composition derived from space-bred desert algae, and a preparation method and application thereof. Background Art

[0002] Space breeding is a relatively efficient artificial breeding method. It uses cosmic radiation, microgravity, and complex electromagnetic environments to induce mutations in microalgae cells, causing genetic mutations in microalgae cells. A large number of mutant strains are obtained at one time, and then strains with fast growth rate, high biomass, strong bioactive ingredients, stable genetic traits, and development value are selected from the mutant strains. Compared with traditional breeding, the biggest advantage of space mutation breeding is the high probability of mutation, wide range of mutation, and short breeding cycle. It can create high-quality germplasm resources in a relatively short period of time. After inquiry, there has never been any report that algae species can survive after space station breeding, nor has it been reported that Hassalli asp. has been used in space breeding research.

[0003] For the Hassalliasp.P06 strain in application number: 202411381158.8, its deposit number is: CCTCCM 20242054. The Hassalliasp.P06 strain was obtained through space breeding and multiple screenings. The strain has the advantages of high photosynthesis efficiency, fast growth rate, strong outdoor adaptability, and high biomass yield.

[0004] Desert algae are a type of algae that can survive in the desert, and Hassallidin A is one of the species of desert algae distributed in desert soil. Experiments have shown that the high content and high activity of CGF (green algae cell growth factor), alkaline polysaccharides, potassium ions, ω-3 unsaturated fatty acids and other ingredients contained in desert algae are helpful in lowering blood lipids and blood pressure and assisting the treatment of cardiovascular and cerebrovascular diseases. A study has isolated a glycosylated lipopeptide, hassallidin A, from the cyanobacteria of the genus Hassallidin, which has strong antifungal activity.

[0005] The desert algae extract in application number: 202311283558.0 has a strong ability to promote the proliferation of skin fibroblasts, can promote the production of cell collagen, significantly improve skin elasticity, and promote the proliferation of fibroblasts. It can be used in the preparation of collagen promoters, agents for improving skin elasticity, agents for improving skin firmness, agents for promoting fibroblast proliferation, or anti-aging agents.

[0006] Cyclic adenosine-3', 5'-monophosphate (cAMP), also known as 3', 5'-cyclic adenosine, is a second messenger in cells. It is formed by the activation of adenylate cyclase to catalyze the cyclization of ATP due to the stimulation of certain hormones or other molecular signals. Chemical formula C 10 H 12 N5O6P, with a molar mass of 329.206 g / mol, is relatively stable, heat-resistant, and is a white or off-white crystal. cAMP acts through protein kinase and plays an important role in regulating sugar, fat, and protein metabolism, gene expression, cell differentiation and proliferation, immune function, and animal production performance.

[0007] The previous inventors discovered the existence of cyclic adenosine monophosphate in the metabolome study of the Hassalliasp.P06 strain; Professor Kandel of Columbia University in the United States found through research that cyclic adenosine monophosphate plays a very important role in repairing brain cells, activating brain cells, and regulating brain cell functions, converting short-term memory into long-term memory, and relieving brain cell fatigue and delaying brain cell aging. Therefore, Kandel won the 2000 Nobel Prize in Medicine, providing technical support for the application of desert algae in skin care. Because there are currently few reports on the efficacy of desert algae for external use on the skin, there are no reports on the extraction of cyclic adenosine monophosphate from desert algae. Due to different algae species, the application number: 202311283558.0 also fails to clarify the anti-aging components of desert algae.

[0008] In view of this, the present invention is proposed in order to broaden the application scope of desert algae. Summary of the invention

[0009] The purpose of the present invention is to provide an anti-aging composition derived from space-bred desert algae, and a preparation method and application thereof.

[0010] The present invention is achieved in that:

[0011] In a first aspect, the present invention provides an anti-aging composition derived from space-bred desert algae, wherein the anti-aging composition derived from space-bred desert algae is an extract of Hassallia sp. P06 bred in space, and the preservation number of Hassallia sp. P06 is CCTCC M 20242054; the components of the anti-aging composition derived from space-bred desert algae include polypeptides, free amino acids, polysaccharides and cyclic adenosine monophosphate, and based on the total mass of the anti-aging composition derived from space-bred desert algae, the content of the polypeptides is 10-63%, the content of the free amino acids is 3-20%, the content of the polysaccharides is 3-20%, and the content of the cyclic adenosine monophosphate is 0.001-0.4%.

[0012] In an optional embodiment, based on the total mass of the anti-aging composition derived from space-bred desert algae, the content of the polypeptide is 18-50%, the content of the free amino acid is 4-15%, the content of the polysaccharide is 4-18%, and the content of the cyclic adenosine monophosphate is 0.008-0.3%;

[0013] Preferably, based on the total mass of the anti-aging composition derived from space-bred desert algae, the content of the polypeptide is 22-36%, the content of the free amino acid is 8-12%, the content of the polysaccharide is 5-12%, and the content of the cyclic adenosine monophosphate is 0.01-0.1%.

[0014] In a second aspect, the present invention provides a method for preparing an anti-aging composition derived from space-bred desert algae as described in any one of the aforementioned embodiments, comprising:

[0015] After subjecting the Hassallia sp.P06 to hydrogen peroxide stress screening, a single strain of the target algae species was obtained;

[0016] The target algae species are cultured on a large scale to obtain Hassallia sp. P06 algae mud;

[0017] Adding water and protease to the Hassallia sp. P06 algae mud, performing enzymolysis for 2-3 hours, then ultrasonically crushing the enzymolysis solution, heating it in a water bath, centrifuging to remove the filter residue, and collecting the Hassallia sp. crude extract;

[0018] The crude extract of Hasa algae is adsorbed by macroporous adsorption resin, the crude extract of Hasa algae after adsorption is centrifuged and filtered, the decolorized Hasa algae extract is collected, and the extract is distilled under reduced pressure to obtain the anti-aging composition derived from the space-bred desert algae.

[0019] In an optional embodiment, the concentration of hydrogen peroxide stress screening is 6×10 -3 ~10×10 -3 mol / L.

[0020] In an optional embodiment, the method for large-scale cultivation includes: placing a single strain of the target algae species in a culture medium to prepare a suspension, inoculating the suspension into water, illuminating with an incandescent lamp with an intensity of 1000 Lux-2000 Lux, culturing with 12 h light / 12 h dark alternation, controlling the temperature between 25° C. and 30° C. during the cultivation process, shaking the culture barrel at regular intervals twice a day, culturing continuously for 14 days, and collecting and cleaning the algae mud of Hassallia sp. P06 when the Hassallia sp. P06 grows to a dry weight of 0.1 g-1 g / L;

[0021] Preferably, the components of the culture medium include 1.5 g / L NaNO3, 0.04 g / L K2HPO4, 0.075 g / LMgSO4·7H2O, 0.0284 g / L CaCl2, 0.006 g / L citric acid, 0.006 g / L ammonium ferric citrate, 0.001 g / L EDTA-Na2, 0.02 g / L Na2CO3 and 1 mL / L A5 solution;

[0022] Preferably, the dry-wet matter specific gravity of the Hassallia sp. P06 algae mud is 60 g to 200 g / L.

[0023] In an optional embodiment, the solid-to-liquid ratio of the Hassallia sp. P06 algae mud to the water is 1 g: 3-5 mL.

[0024] In an optional embodiment, the protease is at least one of alkaline protease, acid protease, neutral protease, flavor protease, trypsin, bromelain, papain and subtilisin;

[0025] Preferably, the power of the ultrasonic crushing is 500-700W, and the time of the ultrasonic crushing is 20-60min;

[0026] Preferably, the water bath heating temperature is 80-85°C, and the water bath heating time is 2-4h. In an optional embodiment, the macroporous adsorption resin is HZ803;

[0027] Preferably, the adsorption comprises adding the enzymatic solution to the macroporous adsorption resin, shaking and mixing for 8-10 hours, and performing static adsorption.

[0028] In a third aspect, the present invention provides an anti-aging composition derived from space-bred desert algae as described in any of the aforementioned embodiments, or an anti-aging composition derived from space-bred desert algae prepared by the method for preparing an anti-aging composition derived from space-bred desert algae as described in any of the aforementioned embodiments, in the preparation of a skin anti-aging agent;

[0029] Preferably, the skin anti-aging agent has at least one of the following uses:

[0030] (1) Hydration and moisturizing;

[0031] (2) Antioxidant properties;

[0032] (3) Improve skin firmness;

[0033] (4) Promote the proliferation of fibroblasts.

[0034] In a fourth aspect, the present invention provides an anti-aging composition derived from space-bred desert algae as described in any of the aforementioned embodiments, or an anti-aging composition derived from space-bred desert algae prepared by the method for preparing an anti-aging composition derived from space-bred desert algae as described in any of the aforementioned embodiments, in the preparation of health products, foods, nutritional enhancers, animal feed or daily chemical products;

[0035] Preferably, the daily chemical products include cosmetics.

[0036] The present invention has the following beneficial effects:

[0037] The present invention provides an anti-aging composition derived from space-bred desert algae, which is derived from an extract of Hassallia sp. P06 bred in space, has a very strong ability to replenish water and retain moisture, can promote the proliferation of skin fibroblasts, significantly improve skin firmness, and has a soothing effect and antioxidant properties. It can be applied to multiple fields such as health products, food, animal feed, cosmetics, and daily chemical products, especially in cosmetics; it can be applied to skin care products or cosmetics as a firming agent or anti-aging agent to improve skin aging symptoms. The preparation method of the anti-aging composition derived from space-bred desert algae is simple. After the Hassallia sp. P06 strain is subjected to hydrogen peroxide stress screening, large-scale culture, water extraction, enzymatic hydrolysis and adsorption, the extract obtained has a high content of effective ingredients. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0039] Figure 1 Schematic diagram of the experimental results of scavenging DPPH free radicals by the extract of Hasa algae;

[0040] Figure 2 This is a schematic diagram of the test results of the extract of Hasa algae promoting the proliferation of fibroblasts;

[0041] Figure 3 This is a schematic diagram of the test results of the effect of the extract of Hasa algae on the shrinkage rate of zebrafish tail length;

[0042] Figure 4 This is a schematic diagram of the test results of the effect of the extract of Hasa algae on the relative expression of the Eln1 gene in zebrafish;

[0043] Figure 5This is a comparison chart of the inhibition rate of elastase in the Hasa algae extract / blank group;

[0044] Figure 6 Schematic diagram of the test results of the effect of Hasa algae extract on the secretion of trypsin in zebrafish larvae. DETAILED DESCRIPTION

[0045] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0046] In a first aspect, the present invention provides an anti-aging composition derived from space-bred desert algae. The anti-aging composition derived from space-bred desert algae is an extract of Hassallia sp. P06 bred in space, and the preservation number of Hassallia sp. P06 is CCTCC M 20242054. The components of the anti-aging composition derived from space-bred desert algae include polypeptides, free amino acids, polysaccharides and cyclic adenosine monophosphate. Based on the total mass of the anti-aging composition derived from space-bred desert algae, the content of polypeptides is 10-63%, the content of free amino acids is 3-20%, the content of polysaccharides is 3-20%, and the content of cyclic adenosine monophosphate is 0.001-0.4%.

[0047] Wherein, based on the total mass of the anti-aging composition derived from space-bred desert algae, the content of polypeptides is 18-50%, the content of free amino acids is 4-15%, the content of polysaccharides is 4-18%, and the content of cyclic adenosine monophosphate is 0.008-0.3%;

[0048] Preferably, based on the total mass of the anti-aging composition derived from space-bred desert algae, the content of polypeptides is 22-36%, the content of free amino acids is 8-12%, the content of polysaccharides is 5-12%, and the content of cyclic adenosine monophosphate is 0.01-0.1%.

[0049] It should be understood that the present invention only limits the active ingredients in the anti-aging composition derived from space-bred desert algae to the above-mentioned polypeptides, free amino acids, polysaccharides and cyclic adenosine monophosphate, and limits their contents, but other ingredients therein are not limited in the present invention, for example, they may be flavonoids, polyphenols, nucleotides and water.

[0050] The present invention provides a method for preparing the anti-aging composition derived from the above-mentioned space-bred desert algae, which comprises the following steps:

[0051] S1. After screening Hassallia sp.P06 under hydrogen peroxide stress, a single strain of the target algae species was obtained.

[0052] In the present invention, Hassallia sp.P06 refers to the Hassallia species collected from the desert of Dalate Banner, Ordos, Inner Mongolia, and sent to the Chinese space station for breeding by the Shenzhou XVI spacecraft on May 30, 2023. It will return to the ground on October 31, 2023, and expand the culture and screening after receiving the sample on November 21, 2023. After 21 generations of continuous culture and screening, the final excellent single strain was obtained, that is, the Hassallia sp.P06 strain of Hassallia in the application number: 202411381158.8. The Hassallia sp.P06 strain of the present invention was deposited in the "China Center for Type Culture Collection" on September 30, 2024, address: Luojia Mountain, Wuchang, Wuhan City, Hubei Province; strain collection number: CCTCC M 20242054. The classification of the Hassalliasp.P06 strain is named Hassalliasp.CODE2.0.

[0053] In the present invention, the above-mentioned Hassallia sp. P06 is further subjected to hydrogen peroxide stress screening, and hydrogen peroxide stress can improve the stress resistance of the Hassallia sp. P06 strain, thereby achieving the antioxidant effect of the algae. The concentration of hydrogen peroxide stress screening is 6×10 -3 ~10×10 -3 mol / L.

[0054] S2, large-scale cultivation of single strains of the target algae species to obtain Hassalliasp.P06 algae mud;

[0055] The method for large-scale cultivation includes: placing a single strain of the target algae species in a culture medium to prepare a suspension, inoculating the suspension into water, illuminating with an incandescent light intensity of 1000 Lux-2000 Lux, culturing with 12 hours of light / 12 hours of darkness, controlling the temperature between 25° C. and 30° C. during the cultivation process, shaking the culture bucket at regular intervals twice a day, culturing continuously for 14 days, and collecting and washing the algae mud of Hassallia sp. P06 when the Hassallia sp. P06 grows to a dry weight of 0.1 g-1 g / L;

[0056] Preferably, the components of the culture medium include 1.5 g / L NaNO3, 0.04 g / L K2HPO4, 0.075 g / LMgSO4·7H2O, 0.0284 g / L CaCl2, 0.006 g / L citric acid, 0.006 g / L ammonium ferric citrate, 0.001 g / L EDTA-Na2, 0.02 g / LNa2CO3 and 1 mL / L A5 solution.

[0057] In an optional embodiment, the dry-wet matter specific gravity of Hassalliasp.P06 algae mud is 60g to 200g / L.

[0058] S3, adding water and protease to the Hassallia sp. P06 algae mud, performing enzymolysis for 2-3 hours, then ultrasonically crushing the enzymolysis solution, heating it in a water bath, centrifuging to remove the filter residue, and collecting the crude extract of Hassallia sp.;

[0059] Specifically, water and protease are added to the Hassallia sp. P06 algae mud according to the solid-liquid ratio of Hassallia sp. P06 algae mud to water of 1g:3-5mL, the protease includes but is not limited to at least one of alkaline protease, acidic protease, neutral protease, flavor protease, trypsin, bromelain, papain and subtilisin, enzymolysis is performed for 2-3h, and then the enzymolysis solution is ultrasonically crushed at a power of 500-700W for 20-60min; heated in a water bath at a temperature of 80-85℃ for 2-4h, and then centrifuged at 4000-5000rpm / min for 10-20min, the filter residue is removed, and the crude extract of Hassallia sp.

[0060] S4. Adsorbing the crude extract of Hasa algae with a macroporous adsorption resin, centrifuging and filtering the adsorbed crude extract of Hasa algae, collecting the decolorized Hasa algae extract, and performing reduced pressure distillation to obtain an anti-aging composition derived from space-bred desert algae.

[0061] Specifically, the macroporous adsorption resin used in the present invention is HZ803. When used, the macroporous adsorption resin is first pre-treated and activated, and then the macroporous adsorption resin is washed with pure water after activation, and the pure water is filtered out and the extract of Hasa algae is added, and the mixture is shaken and mixed for 8-10 hours, and then the mixture is fully subjected to static adsorption. Subsequently, the mixture is centrifuged at 4000-5000rpm / min for 10-20min, the filter residue is removed, the decolorized extract of Hasa algae is collected, and the macroporous adsorption resin is recovered; the anti-aging composition derived from desert algae bred in space is obtained by vacuum distillation and collection.

[0062] The activation method includes: taking a certain amount of macroporous adsorption resin HZ803 and soaking it in anhydrous ethanol for 24 hours, then washing it with pure water until the pH value is neutral; then soaking it in 0.1mol / L NaOH solution with a volume of 2-3 times the volume of the macroporous adsorption resin for 4-5 hours, then washing it with pure water until it is neutral; then soaking it in 0.1mol / L HCl solution with a volume of 2-3 times the volume of the macroporous adsorption resin for 4-5 hours, and finally washing it with pure water until it is neutral. Soak the activated macroporous resin in anhydrous ethanol for standby use. When using it, wash the macroporous resin with pure water.

[0063] The anti-aging composition derived from space-bred desert algae can be widely used in the preparation of skin anti-aging agents; the skin anti-aging agent has at least one of the following uses: (1) moisturizing; (2) having antioxidant properties; (3) improving skin firmness; and (4) promoting the proliferation of fibroblasts.

[0064] The anti-aging composition derived from the above-mentioned space-bred desert algae can also be widely used in the preparation of health products, foods, nutritional enhancers, animal feed or daily chemical products; preferably, daily chemical products include cosmetics. Cosmetics include but are not limited to water-based, emulsion-based, and cream-based. Water-based skin care products include: moisturizing water, repairing water, lotion, makeup remover, etc. Emulsion-based skin care products include: moisturizing lotion, skin care essence, eye essence, facial mask, cleanser, sunscreen, isolation milk, etc.; cream-based skin care products include skin care cream, sunscreen, isolation cream, eye cream, etc.

[0065] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0066] Example 1

[0067] This embodiment provides an anti-aging composition derived from space-bred desert algae, wherein the content of polypeptides, free amino acids, polysaccharides, and cyclic adenosine monophosphate is 18%, 15%, and 0.03%, respectively.

[0068] The present invention is a Hassallia species collected from the desert of Dalate Banner, Ordos, Inner Mongolia, and sent to the Chinese space station for breeding by the Shenzhou XVI spacecraft on May 30, 2023. It will return to the ground on October 31, 2023, and expand culture and screening will be carried out after receiving the sample on November 21, 2023. After 21 generations of continuous culture and screening, the final excellent single strain was obtained, that is, the Hassallia sp.P06 strain of Hassallia in the application number: 202411381158.8. The Hassallia sp.P06 strain of the present invention was preserved in the "China Center for Type Culture Collection" on September 30, 2024, address: Luojia Mountain, Wuchang, Wuhan City, Hubei Province; strain preservation number: CCTCC M 20242054. The classification of the Hassallia sp.P06 strain of Hassallia is named Hassallia CODE2.0.

[0069] The anti-aging composition derived from space-bred desert algae provided in this embodiment is an extract of Hassalliasp.P06 strain, and the extraction method thereof comprises:

[0070] (1) The Hassallia sp. P06 strain was screened under hydrogen peroxide stress. The concentration of hydrogen peroxide was 8×10 -3 mol / L, the culture conditions for stress screening are incandescent light intensity of 1000Lux-2000 Lux, 12h light / 12h dark alternating culture, and the temperature is controlled between 25℃-30℃ during the culture process. The algae species with strong vitality, fast recovery and good growth after stress are selected, and the screening steps are repeated for secondary screening. The target algae species are obtained.

[0071] (2) Cultivation.

[0072] The selected excellent target algae species are cultured on a large scale in culture medium to finally obtain Hasa algae mud.

[0073] The composition of the BG11 culture medium of Hassallia sp.P06 is the same as that of the desert algae culture medium in application number: 202411381158.8, mainly 1.5g / L NaNO3, 0.04g / L K2HPO4, 0.075g / LMgSO4·7H2O, 0.0284g / LCaCl2, 0.006g / L citric acid, 0.006g / L ammonium ferric citrate, 0.001g / L EDTA Na2, 0.02g / L Na2CO3, and 1mL / LA5 solution.

[0074] Use a transparent plastic bucket to fill 50L of distilled water, inoculate 500mL of Hassallia sp.P06 suspension, light intensity of incandescent light is 1000Lux-2000 Lux, and culture is carried out with 12h light / 12h dark alternation. During the culture process, the temperature is controlled between 25℃-30℃. During this period, the culture bucket is shaken twice a day to ensure that Hassallia sp.P06 is evenly distributed in the bucket. After continuous culture for 14 days, when Hassallia sp.P06 grows to 0.1g 1g (dry weight) / L, the Hassallia sp.P06 algae mud is collected and cleaned.

[0075] (3) Extraction and enzymatic hydrolysis

[0076] Pure water and alkaline protease were added at a solid-liquid ratio of 1:4 (g / mL), and the enzymatic hydrolysis was carried out at an appropriate temperature for 3 hours. The enzymatic hydrolyzate was then subjected to 600W ultrasonic crushing for 30 minutes, and then heated in a water bath at 80-85°C for 3 hours; centrifuged at 4500rpm / min for 15 minutes, and the filter residue was removed; and the crude extract of Hasa algae was collected;

[0077] (4) Decolorization and concentration

[0078] Take a certain amount of macroporous adsorption resin HZ803 and soak it in anhydrous ethanol for 24 hours, then wash it with pure water until the pH value is neutral; then soak it in 0.1mol / L NaOH solution with a volume of 3 times the volume of the macroporous adsorption resin for 5 hours, then wash it with pure water until it is neutral; then soak it in 0.1mol / LHCl solution with a volume of 3 times the volume of the macroporous adsorption resin for 5 hours, and finally wash it with pure water until it is neutral. Soak the activated macroporous resin in anhydrous ethanol for standby use. When using, wash the macroporous resin with pure water. Wash the macroporous resin with pure water, filter out the pure water, add the crude extract of Hasa algae, shake and mix for 8 hours, and then fully perform static adsorption; centrifuge at 4500rpm / min for 15 minutes, filter and collect the decolorized Hasa algae extract, and recover the macroporous adsorption resin; vacuum distill and collect the Hasa algae extract, that is, the anti-aging composition.

[0079] Embodiment 2-8

[0080] Examples 2-8 are substantially the same as Example 1, except that different proteases are used in the enzymolysis:

[0081] The protease used in Example 2 is an acidic protease;

[0082] The protease used in Example 3 is a neutral protease;

[0083] The protease used in Example 4 is flavor protease;

[0084] The protease used in Example 5 is trypsin;

[0085] The protease used in Example 6 is bromelain;

[0086] The protease used in Example 7 is papain;

[0087] The protease used in Example 8 was subtilisin.

[0088] Example 9

[0089] The anti-aging composition derived from space-bred desert algae provided in this embodiment is an extract of Hassalliasp.P06 strain, and the extraction method thereof comprises:

[0090] (1) The Hassallia sp. P06 strain was screened under hydrogen peroxide stress. The concentration of hydrogen peroxide was 6×10 -3 mol / L, the culture conditions for stress screening are incandescent light intensity of 1000Lux-2000 Lux, 12h light / 12h dark alternating culture, and the temperature is controlled between 25℃-30℃ during the culture process. The algae species with strong vitality, fast recovery and good growth after stress are selected, and the screening steps are repeated for secondary screening. The target algae species are obtained.

[0091] (2) Same as Example 1.

[0092] (3) Extraction and enzymatic hydrolysis

[0093] Pure water and alkaline protease were added at a material-liquid ratio of 1:3 (g / mL), and the enzymatic hydrolysis was carried out at an appropriate temperature for 2 hours. The enzymatic hydrolyzate was then subjected to 500W ultrasonic crushing for 60 minutes, and then heated in a water bath at 80-85°C for 2 hours; centrifuged at 4000rpm / min for 20 minutes, and the filter residue was removed; and the crude extract of Hasa algae was collected;

[0094] (4) Decolorization and concentration

[0095] Take a certain amount of macroporous adsorption resin HZ803 and soak it in anhydrous ethanol for 24 hours, then wash it with pure water until the pH value is neutral; then soak it in 0.1mol / L NaOH solution with a volume of 3 times the volume of the macroporous adsorption resin for 4 hours, then wash it with pure water until it is neutral; then soak it in 0.1mol / LHCl solution with a volume of 3 times the volume of the macroporous adsorption resin for 4 hours, and finally wash it with pure water until it is neutral. Soak the activated macroporous resin in anhydrous ethanol for standby use. When using, wash the macroporous resin with pure water. Wash the macroporous resin with pure water, filter out the pure water, add the crude extract of Hasa algae, shake and mix for 9 hours, and then fully perform static adsorption; centrifuge at 4000rpm / min for 20 minutes, filter and collect the decolorized Hasa algae extract, and recover the macroporous adsorption resin; vacuum distill and collect the Hasa algae extract, that is, the anti-aging composition.

[0096] Example 10

[0097] The anti-aging composition derived from space-bred desert algae provided in this embodiment is an extract of Hassalliasp.P06 strain, and the extraction method thereof comprises:

[0098] (1) The Hassallia sp. P06 strain was screened under hydrogen peroxide stress. The concentration of hydrogen peroxide was 10×10 -3 mol / L, the culture conditions for stress screening are incandescent light intensity of 1000Lux-2000 Lux, 12h light / 12h dark alternating culture, and the temperature is controlled between 25℃-30℃ during the culture process. The algae species with strong vitality, fast recovery and good growth after stress are selected, and the screening steps are repeated for secondary screening. The target algae species are obtained.

[0099] (2) Same as Example 1.

[0100] (3) Extraction and enzymatic hydrolysis

[0101] Pure water and alkaline protease were added at a solid-liquid ratio of 1:5 (g / mL), and the enzymatic hydrolysis was carried out at an appropriate temperature for 2.5 hours. The enzymatic hydrolyzate was then subjected to 700W ultrasonic crushing for 20 minutes, and then heated in a water bath at 80-85°C for 3 hours; centrifuged at 5000rpm / min for 10 minutes to remove the filter residue; and the crude extract of Hasa algae was collected;

[0102] (4) Decolorization and concentration

[0103] Take a certain amount of macroporous adsorption resin HZ803 and soak it in anhydrous ethanol for 24 hours, then wash it with pure water until the pH value is neutral; then soak it in 0.1mol / L NaOH solution with a volume of 3 times the volume of the macroporous adsorption resin for 5 hours, then wash it with pure water until it is neutral; then soak it in 0.1mol / LHCl solution with a volume of 3 times the volume of the macroporous adsorption resin for 5 hours, and finally wash it with pure water until it is neutral. Soak the activated macroporous resin in anhydrous ethanol for standby use. When using, wash the macroporous resin with pure water. Wash the macroporous resin with pure water, filter out the pure water, add the crude extract of Hasa algae, shake and mix for 10 hours, and then fully perform static adsorption; centrifuge at 5000rpm / min for 10 minutes, filter and collect the decolorized Hasa algae extract, and recover the macroporous adsorption resin; vacuum distill and collect the Hasa algae extract, that is, the anti-aging composition.

[0104] Comparative Example 1

[0105] This comparative example is substantially the same as Example 1, except that the Hassallia sp. P06 in Example 1 is replaced by wild-type Hassallia sp. (commercially available from the Freshwater Algae Seed Bank of the Chinese Academy of Sciences) in this comparative example.

[0106] Comparative Example 2

[0107] This comparative example is substantially the same as Example 1, except that the Hassallia sp. P06 in Example 1 is replaced with slender lamella (commercially available from the Freshwater Algae Seed Bank of the Chinese Academy of Sciences) in this comparative example.

[0108] Comparative Example 3

[0109] This comparative example provides a standard product composition, which includes polysaccharides (standard product: glucose), polypeptides (standard product: casein), free amino acids (standard product: leucine) and cyclic adenosine monophosphate (standard product: cyclic adenosine monophosphate) mixed in a certain proportion.

[0110] Comparative Example 4

[0111] This comparative example is basically the same as Example 1, except that the hydrogen peroxide stress in step (1) is omitted in this comparative example, and the Hassallias p.P06 strain is directly cultured on a large scale.

[0112] Comparative Example 5

[0113] This comparative example is basically the same as Example 1, except that the enzymatic hydrolysis step in step (3) is omitted in this comparative example.

[0114] Comparative Example 6

[0115] This comparative example is basically the same as Example 1, except that the ultrasonic extraction step in step (3) is omitted in this comparative example.

[0116] Experimental Example 1: Detection of the content of active substances in the anti-aging composition

[0117] A. Detection methods of active substances

[0118] (1) Determination of polypeptide content: Protein and Cu in alkaline solution 2+ A purple-red complex is formed, the depth of its color is proportional to the protein content, but has nothing to do with the relative molecular mass and amino acid composition of the protein, so it can be used to determine the protein content.

[0119] Preparation of reagents:

[0120] Standard casein solution (10 mg / mL): The casein protein nitrogen content should be determined in advance by the micro-Kjeldahl method, and the standard solution should be weighed according to its purity. Prepare with 0.05 mol / L NaOH solution.

[0121] Biuret reagent: Take 1.5g CuSO4·5H2O and 6g NaKC4H4O6·4H2O, dissolve in 500mL water, add 300mL 10% NaOH solution under stirring, dilute to 1000mL with water, and store in a plastic bottle (or a bottle with paraffin wax on the inner wall). This reagent can be stored for a long time. If black precipitate appears in the storage bottle, it needs to be re-prepared.

[0122] Preparation of standard curve: Take 6 test tubes, add 0, 0.2, 0.4, 0.6, 0.8, 1mL of standard casein solution respectively, make up to 1mL with water, then add 4mL of biuret reagent to each, shake well, place at room temperature for 30min, and perform colorimetric determination at 540nm. Draw a standard curve with casein content as the horizontal axis and light absorption value as the vertical axis as the basis for quantification.

[0123] Determination of polypeptide content: The same method was used to determine the extract of Hasa algae, and finally the polypeptide content of the sample was calculated.

[0124] (2) Determination of free amino acid content: The free amino group of free amino acids can react with ninhydrin to produce a blue-purple compound, diketoindene-diketoindeneamine. The color depth of the product is proportional to the free amino acid content. The content is measured by a spectrophotometer at 570nm. Because the free amino acids in proteins will also produce the same reaction, they must be removed with a protein precipitant before determination.

[0125] Preparation of reagents:

[0126] Hydrated indoletricopper: Weigh 0.6 g of crystallized indoletricopper, put it into a beaker, add 15 mL of n-propanol to dissolve it, add 30 mL of n-butanol, 60 mL of ethylene glycol, 9 mL of acetic acid-sodium acetate buffer (pH=5.4), mix well, and store in a brown bottle in the refrigerator. It is valid within 10 days.

[0127] Acetic acid-sodium acetate buffer (pH 5.4): Weigh 54.4 g of chemically pure sodium acetate, add 100 mL of ammonia-free distilled water, heat to boiling on an electric furnace to reduce its volume by half, add 30 mL of glacial acetic acid after cooling, and add distilled water to make up to 100 mL.

[0128] Amino acid standard solution: accurately weigh 0.0234 g of leucine dried to constant weight at 80°C and dissolve it in 10% isopropanol and dilute to 50 mL. Take 5 mL of this solution and dilute to 50 mL with distilled water to obtain 5 μg / mL amino acid standard solution.

[0129] 0.1% ascorbic acid: weigh 0.050 g ascorbic acid and dissolve it in 50 mL distilled water. Prepare and use immediately.

[0130] Preparation of standard curve: Take 6 test tubes, add 0, 0.2, 0.4, 0.6, 0.8, 1mL of amino acid standard solution respectively, make up to 2mL with distilled water, then add 3mL of hydrated indene tricopper and 1mL of 0.1% ascorbic acid to each test tube, shake well, seal with stopper and heat in boiling water for 15min, take out and cool quickly with cold water and shake from time to time so that the red color formed during heating is gradually oxidized and faded by air, when it turns blue-purple, use 60% ethanol to make up to 20mL, shake well and measure its absorbance at a wavelength of 570nm. Draw the standard curve with absorbance as the ordinate and amino nitrogen μg as the abscissa.

[0131] Determination of free amino acid content: The same method was used to determine the extract of Hasa algae, and finally the free amino acid content of the sample was calculated. Calculation formula: Amino acid content (mg / g dry sample) = {amino acid μg number × (total volume of extract / measurement volume)} / (sample g number × 1000)

[0132] (3) Determination of polysaccharide content: Polysaccharides are hydrolyzed into monosaccharides under the action of concentrated sulfuric acid, and rapidly dehydrated to form aldehyde derivatives, which are then condensed with phenol to form orange-yellow compounds with stable color. At a wavelength of 488 nm and within a certain concentration range, its absorbance is linearly proportional to the polysaccharide content, so its absorbance can be measured using a spectrophotometer.

[0133] Establishment of standard curve (phenol-sulfuric acid method): Accurately weigh 10 mg of anhydrous glucose reference substance, place it in a 100 mL volumetric flask, add appropriate amount of distilled water to dissolve, dilute to the scale, and shake well. At this time, the glucose concentration is 0.1 mg / mL.

[0134] Take 0, 100, 200, 400, 600, 800, 1000 μL of the above-mentioned reference solution, respectively place them in test tubes, dilute to 1 mL, continue to add 1 mL of 5% phenol solution respectively, shake well, quickly add 5 mL of concentrated sulfuric acid (add slowly), shake well, keep warm in a boiling water bath for 10-15 min, take out, cool to room temperature, use the corresponding reagent as a blank control, measure the absorbance at a wavelength of 488 nm according to the UV-Vis spectrophotometry, and draw a standard curve with the absorbance value as the ordinate and the glucose concentration as the abscissa.

[0135] Determination of polysaccharide content: The same method was used to determine the extract of Hasa algae, and finally the polysaccharide content in the sample was calculated.

[0136] (4) Determination of cyclic adenosine monophosphate content: The cyclic adenosine monophosphate in the sample was extracted with acetonitrile aqueous solution, and the fat was removed by water-saturated n-hexane. The sample was centrifuged and filtered through a membrane. The cyclic adenosine monophosphate was determined by high performance liquid chromatography (HPLC). The retention time was used for qualitative analysis and the peak area was used for quantitative analysis.

[0137] Cyclic adenosine monophosphate standard stock solution: Accurately weigh 0.01 g (accurate to 0.1 mg) of cyclic adenosine monophosphate standard, dissolve it in water and make up to 10 mL, prepare a standard stock solution with a concentration of 1 mg / mL, and store it at 1°C to 4°C. The shelf life is 3 months.

[0138] Determination of cyclic adenosine monophosphate content:

[0139] Weigh 1.0 g (accurate to 0.001 g) of the uniformly mixed sample and place it in a 50 mL stoppered centrifuge tube. Accurately add 25 mL of acetonitrile aqueous solution, vortex mix evenly, perform ultrasonic extraction for 25 min, centrifuge at 6000 r / min for 5 min, take the supernatant and filter it through a microporous membrane before measuring it on the machine.

[0140] Liquid chromatography reference conditions

[0141] 1) Chromatographic column: C18 column, 4.6×250mm, 5μm, or other equivalent chromatographic columns.

[0142] 2) Mobile phase: acetonitrile + formic acid aqueous solution = 5 + 95 (volume ratio).

[0143] 3) Flow rate: 1.0 mL / min.

[0144] 4) Column temperature: 35°C.

[0145] 5) Injection volume: 10 μL.

[0146] 6) Detection wavelength: 254nm.

[0147] Prepare standard curve: dilute the standard stock solution with water to prepare a series of standard solutions with concentrations of 0.5μg / mL, 1μg / mL, 10μg / mL, 20μg / mL, 50μg / mL, and 100μg / mL, which are prepared just before use. Measure according to chromatographic conditions, and draw a standard curve with the peak area of ​​the chromatographic peak as the ordinate and the corresponding solution concentration as the abscissa.

[0148] Determination of samples: The products provided in the examples and comparative examples were measured according to chromatographic conditions, and the retention time was used for qualitative analysis. The concentration of the substance to be measured in the sample solution was obtained based on the measured peak area and the standard curve.

[0149] B. Test results of active substances

[0150] The products provided in the examples and comparative examples were tested according to the above test method to determine the content of components in different examples and comparative examples. The test results are detailed in Table 1.

[0151] Table 1. Component content in different examples

[0152]

[0153]

[0154] As can be seen from the above table, the anti-aging composition derived from space-bred desert algae provided by the present invention is extracted from Hassalliasp.P06 bred in space, and the contents of polypeptides, free amino acids, polysaccharides and cyclic adenosine monophosphate obtained are within the scope of the present invention, and the enzymatic hydrolysis and ultrasound synergistically enhance the efficiency during the extraction process, which can extract the active substances to the maximum extent. The lack of any step cannot achieve the expected effect.

[0155] Experimental Example 2: Efficacy test of anti-aging composition

[0156] (1) Free radical scavenging ability test

[0157] This experimental example uses the DPPH method to determine the free radical elimination ability and antioxidant capacity of the anti-aging composition. The specific test steps are as follows:

[0158] Referring to Table 2, a sample tube (T), sample background (T0), DPPH tube (C) and solvent background (C0) were set up using 10 mL test tubes, and each sample was repeated 3 times. Vitamin E was used as a positive control.

[0159] Table 2 Requirements for adding liquid to test tubes

[0160]

[0161] After adding the reagents according to Table 2, shake gently and react at room temperature in the dark for 30 minutes. Transfer each reaction solution into a 96-well plate and measure the absorbance at 517nm. Figure 1 .

[0162] from Figure 1 It can be seen that the free radical scavenging ability of the Hasa algae extract provided by the present invention is significantly better than that of the wild-type Hasa algae extract, the slender phytoplankton, the standard composition and the positive control group.

[0163] (2) Cell proliferation test

[0164] A. Prepare cell suspension: Digest human immortalized keratinocytes, HaCat, with 2 mL 0.05% trypsin for 2 min, then add 4 mL fresh MEM complete medium to terminate the enzymatic hydrolysis. Centrifuge, remove the supernatant, then add 1 mL fresh MEM complete medium, resuspend the cells, and finally count them using a hemocytometer.

[0165] B. Inoculate into 96-well plates: According to the appropriate number of cells to be plated (about 1×10 4 Each well was filled with about 100 μL of cell suspension, and 6 replicates were set for each concentration.

[0166] C. Culture in a 37°C incubator: After cell inoculation, culture until the confluence reaches 70-80% and then use it for cytotoxicity testing.

[0167] D. After removing the culture medium from the cells, the cells were washed once with phosphate buffer. The concentrations of the Hasa algae extract were first set to 0.01%, 0.1%, 1%, 10% and 100% in sequence, and then different concentrations of the Hasa algae extract were added to a 96-well plate, with 6 replicates for each concentration.

[0168] E. Cultivate in a 37° C. incubator: add the extract of Hasa algae in Example 1 and the extract in the comparative example and culture for 24 hours.

[0169] F. Add MTT solution: Add 10 μL of 5 mg / mL MTT solution to each well and continue incubation for 4 hours. Remove the supernatant and add 100 μL of DMSO to each well. Incubate in a 37°C incubator for 15 minutes to allow the crystals to fully dissolve.

[0170] G. Use an enzyme-linked immunosorbent assay (ELISA) to measure the absorbance of each well at 490 nm.

[0171] H. Analyze data.

[0172] The test results are detailed in Figure 2 ,from Figure 2 It can be seen that with the increase of the concentration of the Hasa algae extract, the cell survival rate first increased, then decreased, and then increased again. In this experimental example, the concentration corresponding to the highest cell survival rate was 0.001%, and the concentration of the subsequent comparative example added was based on the optimal concentration. It can be seen that the effect of the Hasa algae extract provided by the present invention on the proliferation of fibroblasts is significantly better than that of the comparative example.

[0173] (3) Zebrafish Moisture Test

[0174] Wild-type zebrafish with normal development at 72 hpf (72 hours after fertilization) were randomly selected, and a blank control group (no treatment), a model control group (treated with sodium chloride solution), a positive control group (sodium hyaluronate) and a Hasa algae extract (10% of the Hasa algae extract of Example 1, i.e., the Hasa algae extract of Example 1 was diluted 10 times), with 10 fish in each group. First, the zebrafish in each group were placed flat on a glass slide, and then photographed and recorded under a stereo microscope. Subsequently, each group of zebrafish was transferred to a 96-well cell culture plate, 1 per well, and after the water in the well was sucked dry, 200 μL of the corresponding solution of each group was added to each well, and placed in a 28°C constant temperature incubator for incubation for 20 minutes. After incubation for 20 minutes, each group of zebrafish was placed flat on a glass slide again, and photographed and recorded under a stereo microscope. Then, the zebrafish tail length (yolk sac end to caudal vertebrae end) was measured using ImageJ and the zebrafish tail length contraction rate (%) of each group was calculated. The zebrafish tail length shrinkage rate (%) refers to the percentage of the difference in zebrafish tail length before and after dehydration of the test object to the ratio of the tail length before dehydration. The calculation formula is as follows:

[0175] Tail length shrinkage (%) = (Length before dehydration - Length after dehydration) / Length before dehydration × 100%

[0176] Analyze the data and test results. Figure 3 ,from Figure 3 It can be seen that the tail length contraction rate of zebrafish treated with 10% Hassalli asp. extract was 3.86±0.74%, which was 7.33% less than that of the model control group (11.19±0.92%) (p<0.001). Under this experimental condition, the Hassalli asp. extract had an inhibitory effect on the contraction rate of zebrafish tail length (very significant) and had a moisturizing effect.

[0177] (4) Zebrafish firmness test

[0178] Wild-type zebrafish larvae at 4 dpf were collected and placed in a 6-well plate, with 25 larvae in each well. The sample group was treated with 5 mL of sample solution of corresponding concentration, and the last well of 25 larvae was treated with standard dilution water as a blank control group. After treatment, all larvae were collected and washed twice with sterile double distilled water, and then transferred to labeled sterile 15 mL EP tubes, and zebrafish RNA was extracted, and then reverse transcribed into cDNA, and the changes in the mRNA level of the Eln1 gene were detected on a fluorescent quantitative PCR instrument. Data analysis.

[0179] The test results are detailed in Figure 4 ,from Figure 4It can be seen that after treating zebrafish with 2.5% Hassalli asp. extract for 72 hours, the relative expression level of EIn1 gene was 486.82+17.77%, which was 386.82% (<0.001) higher than that of the blank control group, indicating that Hassalli asp. extract has the effect of promoting the expression of EIn1 gene (very significant). It shows that under this experimental condition, Hassalli asp. extract has a firming effect.

[0180] (5) Elastase inhibition test

[0181] A. Add sample solution of the same concentration to the sample tube and sample background. Add pH 8.8 boric acid buffer to the sample background, pH 8.8 boric acid buffer to the enzyme reaction tube, and pH 8.8 boric acid buffer to the solvent background.

[0182] B. Add AAPAN solution, elastase and colorimetric reagent to the sample tube and enzyme reaction tube respectively. Mix the mixture thoroughly and place it in a constant temperature water bath shaker for 20 minutes.

[0183] C. Add pH 6.0 phosphate buffer to the sample tube, sample background, enzyme reaction tube and solvent background respectively, and then use an equal amount of a mixed solution of pH 8.8 boric acid buffer and pH 6.0 phosphate buffer to make the volume 5 mL; centrifuge for 5 minutes and take 3 mL of the supernatant.

[0184] D. Transfer each reaction solution into a 1 cm cuvette and measure the absorbance at 590 nm.

[0185] E. Analyze data.

[0186] The test results are detailed in Figure 5 ,from Figure 5 It can be seen that when the sample concentration is the original solution, the inhibition rate of elastase is 34.18%. Compared with the blank group, the inhibition rate of elastase in the sample group is better than that in the blank group, and there is a significant difference (P<0.05).

[0187] (6) Test of the secretion of trypsin in zebrafish larvae

[0188] Under a stereomicroscope, normally developed 96 hpf (96 hours after fertilization) wild-type zebrafish larvae were randomly selected and placed in a 96-well cell culture plate, 15 per well, and a blank control group (no treatment), a model control group (treated with sodium dodecyl sulfate solution), a positive control group (dipotassium glycyrrhizinate solution) and a test sample group were set up, with 5 parallel groups in each group.

[0189] The soothing water in the 96-well plate was drained, 100 μL of standard dilution water was added to each well of the blank control group, 100 μL of Compound 48 / 80 solution was added to each well of the model control group, 100 μL of sodium cromoglycate solution was added to each well of the positive control group, and 100 μL of sample solution provided in Example 1 of different concentrations was added to each well of the sample group. At the same time, 3 groups of corresponding solutions were set as zero-adjustment control groups and placed in a 28°C constant temperature incubator for incubation for 30 minutes. After incubation for 30 minutes, 50 μL of supernatant was taken in a 96-well plate, 50 μL of BAPNA solution was added to each well, and incubated in a 37°C constant temperature incubator for 1 hour. Five replicate wells were paralleled in each group. After incubation for 1 hour, the absorbance value was measured at a wavelength of 405 nm under the microplate reader and the relative expression level of tryptase was calculated. Analyze the data.

[0190] The test results are detailed in Figure 6 ,from Figure 6 It can be seen that after the zebrafish larvae with allergic reactions induced by Compound 48 / 80 were treated with 1%, 10% and 25% Hassalli asp. extracts, the relative expression levels of tryptase were 7.78±0.18%, -14.00±0.22% and -10.37±0.59%, respectively, which were reduced by 92.22% (p<0.001), 114.00% (p<0.001) and 110.37% (p<0.001) respectively compared with the model control group, indicating that 1%, 10% and 25% Hassalli asp. extracts all have the effect of inhibiting the secretion of tryptase (very significant). It shows that under the conditions of this experiment, 1%, 10% and 25% Hassalli asp. extracts all have a soothing effect.

[0191] In summary, the present invention provides an anti-aging composition derived from space-bred desert algae, which is derived from an extract of Hassallia sp. P06 after space breeding, which has a very strong ability to replenish water and retain moisture, can promote the proliferation of skin fibroblasts, significantly improve skin firmness, and has a soothing effect and antioxidant properties. It can be applied to multiple fields such as health products, food, animal feed, cosmetics, and daily chemical products, especially in cosmetics; it can be used in skin care products or cosmetics as a firming agent or anti-aging agent to improve skin aging symptoms. The preparation method of the anti-aging composition derived from space-bred desert algae is simple. After the Hassallia sp. P06 strain is subjected to hydrogen peroxide stress screening, large-scale culture, water extraction, enzymatic hydrolysis and adsorption, the extract obtained has a high content of effective ingredients.

[0192] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An anti-aging composition derived from space-bred desert algae, characterized in that: The anti-aging composition derived from space-bred desert algae is an extract of Hassalliasp.P06 bred in space, and the preservation number of Hassalliasp.P06 is CCTCC M 20242054; the components of the anti-aging composition derived from space-bred desert algae include polypeptides, free amino acids, polysaccharides and cyclic adenosine monophosphate. Based on the total mass of the anti-aging composition derived from space-bred desert algae, the content of the polypeptides is 10-63%, the content of the free amino acids is 3-20%, the content of the polysaccharides is 3-20%, and the content of the cyclic adenosine monophosphate is 0.001-0.4%.

2. The anti-aging composition derived from space-bred desert algae according to claim 1, characterized in that: Based on the total mass of the anti-aging composition derived from space-bred desert algae, the content of the polypeptide is 18-50%, the content of the free amino acid is 4-15%, the content of the polysaccharide is 4-18%, and the content of the cyclic adenosine monophosphate is 0.008-0.3%; Preferably, based on the total mass of the anti-aging composition derived from space-bred desert algae, the content of the polypeptide is 22-36%, the content of the free amino acid is 8-12%, the content of the polysaccharide is 5-12%, and the content of the cyclic adenosine monophosphate is 0.01-0.1%.

3. A method for preparing an anti-aging composition derived from space-bred desert algae as claimed in any one of claims 1 to 2, characterized in that: It includes: After subjecting the Hassalliasp.P06 to hydrogen peroxide stress screening, a single strain of the target algae species was obtained; The target algae species are cultured on a large scale to obtain Hassalliasp.P06 algae mud; Adding water and protease to the Hassalliasp.P06 algae mud, performing enzymolysis for 2-3 hours, then ultrasonically crushing the enzymolysis solution, heating it in a water bath, centrifuging to remove the filter residue, and collecting the Hassalliasp.P06 crude extract; The crude extract of Hasa algae is adsorbed by macroporous adsorption resin, the crude extract of Hasa algae after adsorption is centrifuged and filtered, the decolorized Hasa algae extract is collected, and the extract is distilled under reduced pressure to obtain the anti-aging composition derived from the space-bred desert algae.

4. The method for preparing the anti-aging composition derived from space-bred desert algae according to claim 3, characterized in that: The concentration of hydrogen peroxide stress screening was 6×10 -3 ~10×10 -3 mol / L.

5. The method for preparing the anti-aging composition derived from space-bred desert algae according to claim 3, characterized in that: The large-scale culture method comprises: placing a single strain of the target algae species in a culture medium to prepare a suspension, inoculating the suspension into water, culturing under an incandescent light with an intensity of 1000 Lux to 2000 Lux, performing 12 hours of light / 12 hours of darkness alternating, controlling the temperature between 25° C. and 30° C. during the culture process, shaking the culture bucket at regular intervals twice a day, culturing continuously for 14 days, and collecting and washing Hassalliasp.P06 algae mud when the Hassalliasp.P06 grows to a dry weight of 0.1 g to 1 g / L; Preferably, the components of the culture medium include 1.5 g / L NaNO3, 0.04 g / L K2HPO4, 0.075 g / L MgSO4·7H2O, 0.0284 g / L CaCl2, 0.006 g / L citric acid, 0.006 g / L ammonium ferric citrate, 0.001 g / L EDTA Na2, 0.02 g / L Na2CO3 and 1 mL / L A5 solution; Preferably, the dry-wet matter specific gravity of the Hassalliasp.P06 algae mud is 60g to 200g / L.

6. The method for preparing the anti-aging composition derived from space-bred desert algae according to claim 3, characterized in that: The solid-to-liquid ratio of the Hassalliasp.P06 algae mud to the water is 1g:3-5mL.

7. The method for preparing the anti-aging composition derived from space-bred desert algae according to claim 3, characterized in that: The protease is at least one of alkaline protease, acidic protease, neutral protease, flavor protease, trypsin, bromelain, papain and subtilisin; Preferably, the power of the ultrasonic crushing is 500-700W, and the time of the ultrasonic crushing is 20-60min; Preferably, the water bath heating temperature is 80-85° C., and the water bath heating time is 2-4 h.

8. The method for preparing the anti-aging composition derived from space-bred desert algae according to claim 3, characterized in that: The macroporous adsorption resin is HZ803; Preferably, the adsorption comprises adding the enzymatic solution to the macroporous adsorption resin, shaking and mixing for 8-10 hours, and performing static adsorption.

9. Use of the anti-aging composition derived from space-bred desert algae as claimed in any one of claims 1 to 2 or the anti-aging composition derived from space-bred desert algae prepared by the method for preparing the anti-aging composition derived from space-bred desert algae as claimed in any one of claims 3 to 8 in the preparation of a skin anti-aging agent; Preferably, the skin anti-aging agent has at least one of the following uses: (1) Hydration and moisturizing; (2) Antioxidant properties; (3) Improve skin firmness; (4) Promote the proliferation of fibroblasts.

10. Use of the anti-aging composition derived from space-bred desert algae as claimed in any one of claims 1 to 2 or the anti-aging composition derived from space-bred desert algae prepared by the method for preparing the anti-aging composition derived from space-bred desert algae as claimed in any one of claims 3 to 8 in the preparation of health products, foods, nutritional enhancers, animal feed or daily chemical products; Preferably, the daily chemical products include cosmetics.

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