A kind of sd rat-derived bone marrow mesenchymal stem cell induction medium and induction method

Abstract

The invention discloses an SD rat-derived bone marrow mesenchymal stem cell induction medium and an induction method. The induction medium is composed of α-MEM medium and additives. In terms of final concentration, the additives are 5-100ng / ml Composition of transforming growth factor-alpha, 10-200ng / ml regulatory protein beta 1, 100-3000nM trehalose and 50-5000nM dibutyryl cyclic adenosine monophosphate. The SD rat-derived bone marrow mesenchymal stem cell induction medium and induction method of the present invention can significantly increase the secretion of BDNF, HGF, VEGF, GDNF and other factors of rat bone marrow mesenchymal stem cells, and the types of secreted factors are also relatively abundant and It can be secreted stably for a long time; the method of cytokine induction avoids the risks and uncertainties brought about by other methods such as genetic modification, and is more suitable for industrialization and clinical application.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an induction medium and an induction method for bone marrow mesenchymal stem cells derived from SD rats. Background technique [0002] With the progress of stem cell research, stem cell transplantation has brought a new dawn for the effective treatment of ischemic stroke. Among various types of stem cells, bone marrow mesenchymal stem cells have become an ideal seed cell for transplantation due to their advantages of convenient material extraction, low immunogenicity, and strong expansion ability. Multiple studies have shown that bone marrow mesenchymal stem cell transplantation can improve neurological dysfunction caused by ischemic stroke to varying degrees. Kvortsova et al. transplanted BM-MSCs into the rat model of focal cerebral ischemia through intravenous injection, and found that the transplanted bone marrow mesenchymal stem cells were mainly distributed in the ische...

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Problems solved by technology

However, the type of factor components after induction is relatively single, only the secretion of GDNF is increased, and it does not increase the secretion of BDNF related to the treatment of ischemic stroke. In addition, the treatment of umbilical cord mesenchymal cells in animal models of ischemic stroke Among them, the effect is not as obvious as that of bone marrow mesenchymal stem cells, and the level of factor secretion induced by its induction scheme is not high, and it cannot be secreted at a high level in a long-term and stable manner.

In addition, low-generation cells are generally used, generally 3-4 generation cells, so the amount of cells that can be induced per unit of primary cells is very limited

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