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Magnetic fluorescent composite nanoparticle, as well as preparation and use thereof

A composite nanoparticle and nanoparticle technology, which is applied in the field of biomedicine and nanomaterials, can solve the problems of difficult control of composite particle size and easy aggregation of magnetic particles, etc., and achieve the reduction of cell magnetic resonance signal, long-lasting fluorescence intensity, and reduced dosage Effect

Inactive Publication Date: 2012-09-26
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, the magnetic particles synthesized in the aqueous phase are easy to agglomerate, and when the quantum dots are embedded in silica at the same time, the particle size of the composite particles is not easy to control, and the near-infrared fluorescent quantum dots coated are mainly limited to thermal therapy applications.

Method used

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  • Magnetic fluorescent composite nanoparticle, as well as preparation and use thereof
  • Magnetic fluorescent composite nanoparticle, as well as preparation and use thereof
  • Magnetic fluorescent composite nanoparticle, as well as preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Hydrophobic Fe 3 o 4 Preparation of nanoparticles: Under nitrogen protection, quickly mix the mixture of 0.5mmol iron acetylacetonate, 2.5mmol 1,2-hexadecanediol, 1.5mmol oleic acid, 1.5mmol oleylamine and 5ml benzyl ether, at 200°C After keeping warm for 2 hours, continue to heat to 300°C, reflux for 1 hour, and cool to room temperature. The product is precipitated with absolute ethanol, centrifuged at 10000rpm / min for 5 minutes, and the precipitate is dissolved in a solution containing 0.013ml oleic acid and 0.013ml oleylamine In 10ml of n-hexane, centrifuge at 6000rpm / min for 10 minutes, and disperse the precipitate in 2ml of n-hexane to obtain 6nm primary hydrophobic Fe 3 o 4 Nanoparticles; Next, the hydrophobic Fe of 10nm is prepared according to the following steps 3 o 4 Nanoparticles: A, containing 20mg 6nm primary hydrophobic Fe 3 o 4 Add 0.5mmol iron acetylacetonate, 2.5mmol 1,2-hexadecane diol, 0.5mmol oleic acid, 0.5mmol oleylamine and 5ml benzyl et...

Embodiment 2

[0040] (1) Hydrophobic Fe 3 o 4 Preparation of nanoparticles: prepare 6nm hydrophobic Fe in the same way as in Example 1 step (1) 3 o 4 particle.

[0041] (2) Hydrophilic Fe 3 o 4 The preparation of nanoparticle: prepare 6nm hydrophilic Fe by the same method of embodiment 1 step (2) 3 o 4 particle.

[0042] (3) Preparation of CdTe fluorescent quantum dots: prepared by the same method as in step (3) of Example 1, the difference being that the reflux time was 10 minutes, and the aqueous dispersion of CdTe fluorescent quantum dots with a fluorescence emission peak of 521nm was obtained.

[0043] (4) Fe 3 o 4 / Preparation of CdTe nanoparticles: take 6nm hydrophilic Fe 3 o 4 Nanoparticles and the quantum dot of fluorescence emission wavelength are 521nm, prepare Fe by the same method of embodiment 1 step (4) 3 o 4 / CdTe nanoparticles.

[0044] (5) Magnetic fluorescent composite nanoparticles Fe 3 o 4 / CdTe / SiO 2 Preparation: Prepared by the same method as in Examp...

Embodiment 3

[0046] (1) Hydrophobic Fe 3 o 4 Preparation of nanoparticles: prepare 8nm hydrophobic Fe in the same way as in Example 1 step (1) 3 o 4 particle.

[0047] (2) Hydrophilic Fe 3 o 4 The preparation of nanoparticle: prepare 8nm hydrophilic Fe by the same method of embodiment 1 step (2) 3 o 4 particle.

[0048] (3) Preparation of CdTe fluorescent quantum dots: prepared by the same method as in Example 1 step (3), the difference being that the reflux time was 1 hour to obtain a CdTe fluorescent quantum dot aqueous dispersion with a fluorescence emission peak of 560 nm.

[0049] (4) Fe 3 o 4 / Preparation of CdTe nanoparticles: take 8nm hydrophilic Fe 3 o 4 The CdTe fluorescent quantum dot of nanoparticle and fluorescence emission wavelength is 560nm, prepares Fe by the same method of embodiment 1 step (4) 3 o 4 / CdTe nanoparticles.

[0050] (5) Magnetic fluorescent composite nanoparticles Fe 3 o 4 / CdTe / SiO 2 Preparation of: Fe 3 o 4 Add 3 ml of ethanol and 50 μl...

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Abstract

The invention discloses a magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 and a preparation method thereof. The method for preparing the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 comprises the steps of: firstly preparing hydrophobic monodisperse Fe3O4 nano-particles by adopting a chemical oil-phase high-temperature method, and modifying the surfaces of the hydrophobic Fe3O4 nano-particles to ensure that the hydrophobic Fe3O4 nano-particles are dispersed in a water phase; preparing luminescent CdTe quantum dots of which the surfaces are provided with carboxyl groups, and precipitating the luminescent CdTe quantum dots on the surfaces of the magnetic Fe3O4 nano-particles through the co-precipitation; then utilizing ligand exchange to modify a silane coupling agent on the surfaces of the luminescent CdTe quantum dots; and finally forming an outermost SiO2 coating layer through silane or silicon ester hydrolysis. The diameter of the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 is between 30 and 50nm; the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2has double functions of magnetism and fluorescence at the same time, has strong and durable fluorescence intensity after labeling rat bone marrow-derived mesenchymal stem cells, and apparently reduces cellular magnetic resonance signals. The particle has broad application prospect in the fields such as biological labeling, bioseparation and the like.

Description

technical field [0001] The invention belongs to the technical fields of nanomaterials and biomedicine, in particular to a magnetic fluorescent composite nanoparticle Fe 3 o 4 / CdTe / SiO 2 And its preparation method and application. Background technique [0002] In molecular imaging, nanoparticles with superparamagnetic iron oxide as the core have become one of the main technical means for magnetic resonance molecular imaging research. Magnetic resonance imaging (MRI) has the advantages of no damage, high spatial resolution, and multi-dimensional observation. Stem cell research, which has developed rapidly in recent years, is also widely mediated by various transfection reagents (such as polylysine (PLL), protamine sulfate, cationic liposomes, etc.) or ligand-receptor, antigen-antibody reactions. Iron oxide nanoparticles label stem cells for in vitro or in vivo imaging purposes. A number of studies have proved that iron oxide nanoparticles can successfully label stem cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/88
Inventor 聂立波张黎明颜荣华单鸿
Owner SUN YAT SEN UNIV
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