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Effective Fractions of Buyang Huanwu Decoction and Its Application

A technology of effective parts and supplementing yang, applied in the field of medicine, can solve the problems of complex components, multiple effects, and multiple targets of traditional Chinese medicine prescriptions, and achieve the effect of promoting the differentiation of bone marrow mesenchymal stem cells

Active Publication Date: 2020-04-03
郑景辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composition of traditional Chinese medicine prescriptions is complex, and its function has the characteristics of "multi-causes, multi-effects, and multi-targets"

Method used

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  • Effective Fractions of Buyang Huanwu Decoction and Its Application
  • Effective Fractions of Buyang Huanwu Decoction and Its Application
  • Effective Fractions of Buyang Huanwu Decoction and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] An application of effective fractions of Buyang Huanwu Decoction in inducing differentiation of bone marrow mesenchymal stem cells into nerve cells, wherein the effective fractions of Buyang Huanwu Decoction are: glycosides, total alkaloids, angelica polysaccharides, red Anthocyanin and naringenin; the preparation method steps of effective parts of Buyang Huanwu Decoction are as follows:

[0035] (1) Separation and purification of glycoside compounds and effective parts of naringenin: Take 60g of Radix Astragali, 6g of Radix Paeoniae Rubra, and 9g of Earthworm, add 5-15 times of 40%-70% ethanol, reflux extraction 1-3 times, each time 1 -2h, filter to get medicinal dregs A, and set aside; the filtrate recovers ethanol and adjusts it to a concentrated solution equivalent to 0.5-3g medicinal materials per 1ml. After the concentrated solution is absorbed by a macroporous resin column, it is washed with water, and then washed with 40-50% Elution with ethanol, collect the eth...

Embodiment 2

[0039] An application of effective fractions of Buyang Huanwu Decoction in inducing differentiation of bone marrow mesenchymal stem cells into nerve cells, wherein the effective fractions of Buyang Huanwu Decoction are: glycosides, total alkaloids, angelica polysaccharides, red Anthocyanin and naringenin; the preparation method steps of effective parts of Buyang Huanwu Decoction are as follows:

[0040] (1) Separation and purification of glycoside compounds and effective parts of naringenin: Take 60g of Radix Astragali, 6g of Radix Paeoniae Rubra, and 9g of Earthworm, add 5-15 times of 40%-70% ethanol, extract by percolation, collect the leachate, and The slag A is used for later use; ethanol is recovered from the leachate, and adjusted to a concentrated solution equivalent to 0.5-20g of medicinal materials per 1ml. % ethanol elution, eluent 100ml as a fraction, high performance liquid phase method to monitor the dynamic changes of the elution fraction components, fractions wi...

Embodiment 3

[0044] The effective part group of Buyang Huanwu Decoction promotes the differentiation of bone marrow mesenchymal stem cells into neuron-like cells in vitro, and the role analysis of MAPK in it.

[0045] Material:

[0046] Animals: 10 clean-grade male SD rats aged 6-8 weeks, body weight (130±20) g, purchased from Shanghai Slack Experimental Animal Co., Ltd., animal license number: SCXK (Shanghai) 2009-0004. The temperature of the animal room was 25°C and the humidity was 60%. The operation of animals in the rat experiment was carried out in strict accordance with the relevant regulations in the "Guiding Opinions on the Kindness of Experimental Animals" promulgated by the Ministry of Science and Technology of the People's Republic of China.

[0047] Drug: Astragalus (Latin scientific name: Leguminosae ) 60g, Paeonia lactiflora (Latin scientific name: Paeonia lactifloraPall.) 6g, Chuanxiong (Latin scientific name: Rhizoma Chuanxiong) 6g, Angelica (scientific name: Angelica s...

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Abstract

The invention belongs to the technical field of medicine and relates to uses of various traditional Chinese medicine mixed compounds, in particular to application of an effective part group of yang-tonifying recuperating decoction in inducing mesenchymal stem cells to differentiate into nerve cells.The effective part group includes effective parts extracted from medicinal materials, including glucoside compounds, total alkaloids, Angelica sinensis polysaccharide, carthamin yellow and naringenin.Researches show that this effective part group can induce in vitro rat mesenchymal stem cells to differentiate into neuron-like cells and is applicable to drugs for treating nervous system diseases.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to the use of various traditional Chinese medicine mixed compounds, in particular to a group of effective fractions of Buyang Huanwu Decoction and its application in inducing bone marrow mesenchymal stem cells to differentiate into nerve cells. Background technique [0002] Bone marrow mesenchymal stem cells (BMSCs) are non-hematopoietic cells derived from bone marrow tissue. They are easy to obtain, not subject to ethical restrictions, have a wide range of proliferation and differentiation capabilities, and are easy to isolate, culture, expand, and purify. , weak immunogenicity, strong immune tolerance, high gene transfection rate in vitro, and stable and efficient expression of foreign genes. Therefore, MSCs become an ideal seed cell. In recent years, studies have reported that MSCs can differentiate into neuron-like cells in vivo. In vitro, basic fibroblast factor (bFGF ), dimerc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/736A61P25/00A61K35/62
CPCA61K9/0095A61K35/62A61K36/232A61K36/236A61K36/286A61K36/481A61K36/65A61K36/71A61K36/736A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55A61K2300/00
Inventor 郑景辉
Owner 郑景辉
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