Drug for inducing bone marrow mesenchymal stem cell (MSC) differentiation to cardiomyocyte and detection method thereof

A cardiomyocyte-like cell and stem cell differentiation technology, applied in bone/connective tissue cells, biochemical equipment and methods, animal cells, etc., can solve the problems of low conversion rate of MSCs, poor effect, and few types of drugs, so as to improve the success rate , the effect of reducing mortality
CN109679897APending Publication Date: 2019-04-26ZHEJIANG CANCER HOSPITAL
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
ZHEJIANG CANCER HOSPITAL
Publication Date
2019-04-26

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Abstract

The invention belongs to the technical field of traditional Chinese medicines, discloses a drug for inducing bone marrow mesenchymal stem cell (MSC) differentiation to cardiomyocyte and a detection method thereof. The method includes: preparation of drug serum of Zhejiang radix ophiopogonis; preparation of cardiomyocyte extraction solution and lysate; separation and culture of rat MSCs; identification of surface markers of rat MSCs by a flow cytometer; culture of rat MSCs; detection of the expression of cardiomyocyte-associated proteins CTnI and CX43 by immunofluorescence; and detection of theexpression of NKx2.5, GATA-4 and beta-MHC mRNA by a qPCR method. The method disclosed by the invention establishes a high-efficiency and systematic research mode for batch searching and research of traditional Chinese medicine with potential inducibility, lays a foundation for perfecting the screening and development system of traditional Chinese medicine inducer, provides a new idea for the research of traditional Chinese medicine induction, has great significance for improving the success rate of autologous transplantation of MSCs, reducing the mortality of patients, and preventing the occurrence and development of diseases such as coronary heart failure.
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Description

technical field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a medicine for inducing bone marrow mesenchymal stem cells to differentiate cardiomyocytes and a detection method. Background technique

[0002] At present, the existing technologies commonly used in the industry are as follows:

[0003] Cardiovascular disease is one of the most important diseases that threaten human health in the world today, and its morbidity and mortality have surpassed tumor diseases and ranked first in the world. According to modern research, there are a variety of clinical cardiovascular diseases that pose a great threat to human life, such as coronary heart disease and heart failure, all caused by myocardial tissue damage and necrosis. This is due to the lack of regenerative ability of mature cardiomyocytes in myocardial tissue. After myocardial ischemia and infarction, cardiomyocytes (CM) will be damaged and die in a lar...

Claims

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