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Drug for inducing bone marrow mesenchymal stem cell (MSC) differentiation to cardiomyocyte and detection method thereof

A cardiomyocyte-like cell and stem cell differentiation technology, applied in bone/connective tissue cells, biochemical equipment and methods, animal cells, etc., can solve the problems of low conversion rate of MSCs, poor effect, and few types of drugs, so as to improve the success rate , the effect of reducing mortality

Pending Publication Date: 2019-04-26
ZHEJIANG CANCER HOSPITAL
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Problems solved by technology

[0009] (1) The conversion rate of MSCs is low during the transplantation process of using MSCs autologous transplantation to treat myocardial injury
[0010] (2) Existing drugs that induce MSCs to differentiate into CM are few in variety, poor in effect, and highly toxic

Method used

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  • Drug for inducing bone marrow mesenchymal stem cell (MSC) differentiation to cardiomyocyte and detection method thereof
  • Drug for inducing bone marrow mesenchymal stem cell (MSC) differentiation to cardiomyocyte and detection method thereof
  • Drug for inducing bone marrow mesenchymal stem cell (MSC) differentiation to cardiomyocyte and detection method thereof

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Embodiment Construction

[0086] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0087] Taking Zhejiang Ophiopogon japonicus as an example, the present invention provides a method for inducing heart-invigorating traditional Chinese medicine to differentiate cardiomyocyte-like cells from bone marrow mesenchymal stem cells. The present invention uses flow cytometry to identify the surface marker molecules CD73, CD90, and CD45 of rat bone marrow MSCs , CD34; Observing the effect of Zhe Mai Dong, a traditional Chinese medicine for nourishing heart yin, on the morphology and structure of cardiomyocytes induced by SD rat MSCs; detection of CTnI and CX43 protein expression; qPCR method to detect the expression...

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Abstract

The invention belongs to the technical field of traditional Chinese medicines, discloses a drug for inducing bone marrow mesenchymal stem cell (MSC) differentiation to cardiomyocyte and a detection method thereof. The method includes: preparation of drug serum of Zhejiang radix ophiopogonis; preparation of cardiomyocyte extraction solution and lysate; separation and culture of rat MSCs; identification of surface markers of rat MSCs by a flow cytometer; culture of rat MSCs; detection of the expression of cardiomyocyte-associated proteins CTnI and CX43 by immunofluorescence; and detection of theexpression of NKx2.5, GATA-4 and beta-MHC mRNA by a qPCR method. The method disclosed by the invention establishes a high-efficiency and systematic research mode for batch searching and research of traditional Chinese medicine with potential inducibility, lays a foundation for perfecting the screening and development system of traditional Chinese medicine inducer, provides a new idea for the research of traditional Chinese medicine induction, has great significance for improving the success rate of autologous transplantation of MSCs, reducing the mortality of patients, and preventing the occurrence and development of diseases such as coronary heart failure.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a medicine for inducing bone marrow mesenchymal stem cells to differentiate cardiomyocytes and a detection method. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Cardiovascular disease is one of the most important diseases that threaten human health in the world today, and its morbidity and mortality have surpassed tumor diseases and ranked first in the world. According to modern research, there are a variety of clinical cardiovascular diseases that pose a great threat to human life, such as coronary heart disease and heart failure, all caused by myocardial tissue damage and necrosis. This is due to the lack of regenerative ability of mature cardiomyocytes in myocardial tissue. After myocardial ischemia and infarction, cardiomyocytes (CM) will be damaged and die in a lar...

Claims

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Application Information

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IPC IPC(8): C12N5/077C12Q1/02C12Q1/6888G01N33/68
CPCC12N5/0657C12N2500/76C12N2506/1353C12Q1/6888C12Q2600/158G01N33/5008G01N33/68
Inventor 章红燕李清林蒋卉章锃宇李骏飞姜建伟周佳佳王琼郑小蓉
Owner ZHEJIANG CANCER HOSPITAL
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