Immortalized rat marrow stroma cell system and preparation method thereof

A bone marrow stromal cell, immortalization technology, applied in biochemical equipment and methods, botanical equipment and methods, cells modified by introducing foreign genetic material, etc. Achieve strong proliferation ability, cell line stability, in vitro and in vivo induction and differentiation promotion effects
CN101255407AInactive Publication Date: 2008-09-03CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
Publication Date
2008-09-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a immortal stromal cell systme of rat's marrow and preparation thereof. Immortal stromal cell of rat's marrow can be obtained in short time by the inventive method. The inventive hTERT immortal stromal cell systme of rat's marrow is stable, can be sequentially passed through generations and keep diploid karyotype and characteristics of normal stromal cell of rat's marrow, has extremely strong proliferation ability, and realize immortalization. Immortal stromal cell system of rat's marrow established by the inventive method can conveniently and quickly obtain large amount of stromal cell of rat's marrow with diploid karyotype. This no doubt has great promotion to research of introduction and bone marrow stromal cell in vitro and in vivo.
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Description

technical field

[0001] The invention relates to an immortalized rat bone marrow stromal cell line and a preparation method thereof. Background technique

[0002] Bone marrow stromal cells (bMSCs) are a kind of stem cell population with multilineage differentiation potential existing in bone marrow. Under certain conditions, bMSCs can differentiate into bone cells, chondrocytes, cardiomyocytes, hepatocytes, neurons and glial cells in vitro or in vivo. Bone marrow stromal cells have the advantages of rich sources, convenient collection, weak immunogenicity, low bacterial contamination rate, and no ethical issues, which provide a new method for cell transplantation or replacement therapy of Parkinson's disease (PD) and other degenerative diseases. A new source of seed cells. It is precisely because the cells have multi-directional differentiation potential, and can realize autologous materials and autologous transplantation, thereby avoiding immune rejection, so bMSCs have be...

Claims

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