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Method for rapidly detecting content of aflatoxin

A technology of aflatoxin and content, which is applied in the direction of material analysis, fluorescence/phosphorescence, and chemical reaction of materials by observing the effect on chemical indicators, which can solve the problems of high detection cost and complex detection steps. , to achieve the effect of overcoming hazards and pollution, reducing detection limit and improving sensitivity

Inactive Publication Date: 2011-01-19
云南健牛环境监测有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this type of kit still has problems such as high detection cost and complicated detection steps.

Method used

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  • Method for rapidly detecting content of aflatoxin
  • Method for rapidly detecting content of aflatoxin
  • Method for rapidly detecting content of aflatoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Detect whether the aflatoxin B1 in the piglet's compound feed exceeds the standard. According to GB13078-2001 "Feed Hygienic Standards", the compound feed and concentrated feed for meat piglets should be less than or equal to 10 μg / kg. Sample Mg calculation formula: Mg=(0.04 μg / ml×1 ml) / 10 μg / kg, Mg=4g. Take 4g of the sample to be tested and grind it through a 20-mesh sieve into a 100ml beaker, add 10ml of chloroform solution, shake for 2min, and filter with qualitative filter paper. Heat the filtrate in a water bath at 70°C to concentrate, volatilize to about 0.5ml, and dilute to 1ml with chloroform (referred to as the test sample). On a thin-layer silica gel plate with a length of 10 cm, add 30 μl each of the test sample and aflatoxin B1 standard substance (5 μl each time, 6 times in total), and then add oxalic acid with a concentration of 1.5% by weight and volume. 10 μl was used as derivatization reagent. After evaporating to dryness, put it into a chromatography...

Embodiment 2

[0030] Detect whether the aflatoxin B1 in the compound feed of 212 growing chickens exceeds the standard. According to GB13078-2001 "Feed Hygienic Standards", the compound feed and concentrated feed for broiler chicks, growing chickens, and laying hens are ≤20μg / kg. Sample Mg calculation formula: Mg=(0.20μg / ml×1ml) / 20μg / kg, Mg=10g. Take 10 g of the sample to be tested and grind it through a 20-mesh sieve into a 100 ml beaker, add 20 ml of dichloromethane solution, shake for 5 minutes, and filter with quantitative filter paper. Heat the filtrate in a water bath at 65°C to concentrate, volatilize to about 1ml, and dilute to 2ml with dichloromethane (referred to as the test sample). Add 40 μl each of the test sample and aflatoxin standard B1 on a thin-layer silica gel plate with a length and width of 12 cm (5 μl each time, 8 times in total), and then add 3% dilute hydrochloric acid as a derivative reagent 15 μl. After evaporating to dryness, put it into a chromatographic cylin...

Embodiment 3

[0032] Detect whether aflatoxin B1 in corn exceeds the standard. According to GB2715-2005 "Food Hygienic Standards", the maximum allowable content of aflatoxin B1 in corn is ≤20μg / kg. Sample Mg calculation formula: Mg=(0.20μg / ml×1ml) / 20μg / kg, Mg=10g. Take 10 g of the sample to be tested and grind it through a 20-mesh sieve, put it in a 100 ml beaker, add 30 ml of isopropanol solution and ultrasonicate for 5 minutes, and filter with qualitative filter paper. Heat the filtrate in a water bath at 80°C to concentrate, volatilize to about 2ml, and dilute to 3ml with isopropanol (referred to as the test sample). Spot 24 μl each of the test sample ① and standard B1 on a thin-layer silica gel plate with a length of 15 cm (6 μl for each spotting, 4 times in total), and then add 5 μl of 2% derivative reagent sulfuric acid. After evaporating to dryness, put it into a hexane chromatography tank filled with solvent for a first development, the solvent front is developed for 10 cm, and th...

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Abstract

The invention discloses a method for rapidly detecting the content of aflatoxin. The method comprises the following steps of: after smashing a sample to be detected, putting the sample powder into a beaker, and adding an extractant for shaking or performing ultrasonic extraction; filtering, performing water bath evaporation on a filtrate, and metering the volume to obtain a test sample; respectively pointwise adding the test sample and an aflatoxin B1 standard substance which are commensurate on a thin-layer silica gel plate, pointwise adding derivative reagents into sampling points, and respectively developing by developers; and comparing the fluorescence intensity of the test sample and the aflatoxin B1 standard substance under an ultraviolet lamp to judge whether the content of the aflatoxin in the test sample exceeds the standard. The method for rapidly detecting the content of the aflatoxin has the advantages of small solvent amount, simple sample treatment and short measurement time; the method does not need a professional, only needs small apparatus, and is particularly suitable for rapid field detection; and the method effectively avoids the harm and the pollution of trifluoroacetic acid to operators and the environment, improves the method sensitivity, and has the lowest detectable amount of 0.00032 mu g and the lowest detection limit of 3 mu g / kg.

Description

technical field [0001] The invention belongs to the technical field of food safety inspection, and in particular relates to a method for quickly detecting the residual content of aflatoxin in food and feed. Background technique [0002] Aflatoxins are metabolites produced by Aspergillus flavus and Aspergillus parasiticus. When grains are not dried in time or stored improperly, they are often easily contaminated by Aspergillus flavus or Aspergillus parasiticus and produce such toxins. The incidence of aflatoxins in food and feed in hot and humid regions is high. Aflatoxins mainly contaminate feed, grain and oil foods, animal and plant foods, etc.; such as feed, peanuts, corn, rice, wheat, beans, nuts, peppers, etc. will all be contaminated with aflatoxins. Among them, feed, chili, peanut and corn are the most polluted. And when people eat food contaminated by aflatoxin by mistake, it will cause harm to their health. At present, it is still relatively difficult to prevent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/77G01N21/78G01N21/64
Inventor 杨亚玲吕金玲李冉
Owner 云南健牛环境监测有限公司
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