Aflatoxin nano antibody gene pool, construction method and application of aflatoxin nano antibody gene pool as well as aflatoxin B1 nano antibody 2014AFB-G15

A 2014AFB-G15, aflatoxin technology, applied in genetic engineering, plant genetic improvement, chemical instruments and methods, etc., to achieve good stability, reduce production costs, and reduce interference effects
CN103866401AActive Publication Date: 2014-06-18INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Publication Date
2014-06-18
Patent Text Reader

Abstract

The invention relates to an aflatoxin nano antibody gene pool, a construction method and application of the aflatoxin nano antibody gene pool as well as an aflatoxin B1 nano antibody 2014AFB-G15. The aflatoxin nano antibody gene pool is prepared by extracting RNA (ribonucleic acid) in alpaca blood after immunization of an aflatoxin B1 antigen, performing specific amplification on a variable region gene of an alpaca heavy chain antibody by adopting an RT-PCR (reverse transcription-polymerase chain reaction) method to obtain an aflatoxin nano antibody VHH gene, and then performing transformation after connecting with a pCANTAB5E (his) vector. The aflatoxin B1 nano antibody 2014AFB-G15 obtained by screening, disclosed by the invention, has the characteristics of organic reagent resistance, high temperature resistance and the like, and is good in stability; the IC50 (half maximal inhibitory concentration) of the aflatoxin B1 nano antibody 2014AFB-G15 to the aflatoxin B1 is 0.66ng / mL, and the cross reactivity of the aflatoxin B1 nano antibody 2014AFB-G15 to the aflatoxins B2, G1,G2 and M1 is 22.6%, 0.95%, 32.1% and 26% respectively.
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Description

technical field

[0001] The invention relates to aflatoxin nanobody gene library, construction method, application and aflatoxin B1 nanobody 2014AFB-G15. Background technique

[0002] Aflatoxins are mainly secondary metabolites secreted by Aspergillus flavus and Aspergillus parasiticus, and are natural toxic compounds that can cause various damages to humans and animals. More than 20 kinds of aflatoxins have been found so far, among which aflatoxin B1 is the most toxic, its toxicity is 10 times that of potassium cyanide and 68 times that of arsenic. As early as 1993, aflatoxin B1 was designated as one of the strongest known carcinogenic chemicals by the Cancer Research Institute of the World Health Organization, that is, Class I carcinogens. my country is an area with heavy aflatoxin pollution, and aflatoxin pollution is likely to exist in various foods and agricultural products, especially corn, peanuts and their products. Therefore, it is of great significance to strength...

Claims

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