Bacillus licheniformis agent for preventing and treating black shank and preparation method thereof

A technology for Bacillus licheniformis and tobacco black shank, which can be applied to botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., and can solve problems such as poor control effect, increased control difficulty, and environmental pollution. , to achieve the effect of reducing the amount of use, reducing the harm of pesticides, and effective inoculants

Active Publication Date: 2009-03-18
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical control not only has high cost and poor control effect, but also pollutes the environment and affects the quality, and some pathogenic fungi show resistance to metalaxyl commonly used in production, which makes the control effect worse and the control difficulty is increased

Method used

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  • Bacillus licheniformis agent for preventing and treating black shank and preparation method thereof
  • Bacillus licheniformis agent for preventing and treating black shank and preparation method thereof
  • Bacillus licheniformis agent for preventing and treating black shank and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] ——Preparation Method 1 of Liquid Fermentation Inoculant

[0037] (1) Strain preparation

[0038] Move Bacillusl licheniformis GP13 to test tube slant medium (3g of beef extract, 5g of soybean peptone, 5g of glucose, 17-20g of agar, 1000ml of distilled water, pH6.8-7.2), and incubate at 30°C for 1-2 days to obtain test tube species.

[0039] (2) Liquid fermentation

[0040] Add 10ml of sterile water to the slant of each test tube, scrape off the bacterial lawn with an inoculation loop, and shake up to form a bacterial suspension, insert sterilized and cooled to 30°C with 0.5% volume ratio, and 200ml culture solution (soybean Peptone 10g, sucrose 5g, yeast extract 2g, potassium dihydrogen phosphate 1g, ferrous sulfate 0.02g, zinc sulfate 0.02g, manganese sulfate 0.02g, magnesium sulfate 0.3g, tap water 1000ml, peanut oil 0.1%, defoamer 0.01% , pH7.0-7.2) in a 500ml Erlenmeyer flask, cultured at 30°C for 36 hours at a constant temperature of 150-200 rev / min with shaking...

Embodiment 2

[0044] ——Preparation method of liquid fermentation agent 2

[0045] (1) Strain preparation

[0046] Move Bacillusl licheniformis GP13 to a 150ml eggplant-shaped flask slant medium (beef extract 3g, soybean peptone 5g, glucose 5g, agar 17-20g, distilled water 1000ml, pH 6.8-7.2), and culture at a constant temperature of 30°C In 1-2 days, the slant strains were obtained.

[0047] (2) Liquid fermentation

[0048] Add 100ml of sterile water to the slope of each eggplant-shaped bottle, scrape off the bacterial lawn with an inoculation loop, and shake up to form a bacterial suspension, and insert the sterilized liquid culture solution (soybean) cooled to 30°C with a volume ratio of 0.5%. Peptone 10g, sucrose 5g, yeast extract 2g, potassium dihydrogen phosphate 1g, ferrous sulfate 0.02g, zinc sulfate 0.02g, manganese sulfate 0.02g, magnesium sulfate 0.3g, tap water 1000ml, pH 7.0-7.2) in a fermentation tank , according to the liquid fermentation conditions of the present invention...

Embodiment 3

[0052] ——Preparation method of solid microbial agent 1

[0053] The liquid bacterial agent obtained by the liquid fermentation bacterial agent preparation method 1 is added 1:1 diatomite and light calcium carbonate as a filler according to 50% of the liquid, vacuum-filtered with No. 7 filter cloth, ventilated and dried in the shade, and pulverized by a high-speed universal pulverizer , 100g / bag into plastic or aluminum plastic bags. Its packaging should undergo strict inspection and meet the packaging standards for wettable powder pesticides.

[0054] Determination of finished product quality indicators: the determination of bacteria content, water content, suspension rate and fineness are all carried out according to the national enterprise standard (Q / KWL02-2003), the bacteria content is 20 billion / g, and other indicators meet the standards.

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Abstract

The invention discloses a microbial inoculum for preventing the black shank and a preparation method thereof. The classification of production strain of the microbial inoculum is named as bacillus licheniformis GP13, and the preservation number is CGMCCNo.1699. The formulations of the solid medium and the liquid medium of the microbial inoculum are as follows: (1) the formulation of the medium of test tube slant is as follows: 3g of beef extract, 5g of soy peptone, 5g of glucose, 17g to 20g of agar and 1,000ml of distilled water are needed, and the pH is 6.8 to 7.2; (2) the formulation of the medium of liquid fermentation is as follows: 20g of soy peptone, 5g of sucrose, 2g of yeast extract paste, 1g of monopotassium phosphate, 0.02g of ferrous sulfate, 0.02g of zinc sulfate, 0.02g of manganese sulfate, 0.02g of magnesium sulfate, 1,000ml of water, 0.1 percent of peanut oil and 0.01 percent of an antifoaming agent, and the pH is 7.0 to 7.2. The result of the test proves that the invention has good application prospect for preventing the black shack.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a bacterial agent for preventing and treating tobacco black shank and a preparation method of the bacterial agent. Background technique [0002] Tobacco is an important economic crop in my country, and it is also one of the crops with the most serious disease damage. From sowing to harvesting, the whole process can be infected by pathogens. Tobacco blackshank (tobacco blackshank) is one of the most serious diseases in the world's tobacco production, especially in temperate, subtropical and tropical regions. It is the main disease of tobacco in my country. In my country, except for a few colder regions, the major tobacco-producing provinces have different degrees of occurrence. Among them, Anhui, Henan, and Shandong are the severe disease areas in history; Yunnan, Guizhou, Fujian, Guangdong, Hunan, Sichuan and other smoking areas are also quite common, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P3/00C12N1/20C12R1/10
Inventor 方敦煌邓建华邓云龙宋春满
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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