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Method for producing 1,3-dioleoyl-2-palmitoyl triglyceride

A technology of palmitic acid triglyceride and production method, which is applied in the direction of fatty acid esterification, fat production, edible oil/fat, etc., and can solve problems such as increased cholesterol content, increased risk of cardiovascular disease, and unfavorable health

Inactive Publication Date: 2011-11-02
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although cholesterol is an indispensable nutrient for the human body, it is not only a component of cell membranes, but also a raw material for the synthesis of many important substances such as steroid hormones and bile acids, but excessive cholesterol in triglycerides or long-term intake of cholesterol High fat will increase the cholesterol content in serum, which is not conducive to health and increases the risk of cardiovascular disease

Method used

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  • Method for producing 1,3-dioleoyl-2-palmitoyl triglyceride
  • Method for producing 1,3-dioleoyl-2-palmitoyl triglyceride
  • Method for producing 1,3-dioleoyl-2-palmitoyl triglyceride

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1 combined figure 1 illustrate

[0038] Step 1 Lard raw material is obtained by boiling fat and suet from commercially available healthy pigs over medium heat; heat the boiled lard to about 32°C, add 2% (w / w) β-cyclodextrin, Then add 40% (w / v) ethanol aqueous solution (80%), vigorously shake and mix, then let stand for 30 minutes, discard the water phase in layers, and extract the oil phase twice according to the above process, the results are as follows:

[0039]

[0040] Step 2 Add 2 mol / L NaOH-ethanol solution to commercially available olive oil, reflux at 80°C for 4 hours, extract twice with n-hexane to remove unsaponifiable matter; separate the n-hexane phase, add 1 mol / L hydrochloric acid to the water phase to acidify to pH 1 -2, add n-hexane to extract the organic phase for 3 times, discard the water phase, add activated clay to the n-hexane phase for decolorization, remove the activated clay by filtration, remove the solvent, dehydrate and dry to ob...

Embodiment 2

[0049] Example 2 combined figure 1 Description, step 1-2 is the same as embodiment 1

[0050] Step 3-4 Put the lard decholesterolated in the above step 1 and the fatty acid refined in the step 2 into the tank 1 at a ratio of 0.5:1, heat to 42°C, dissolve and mix well, and then pump into the above reactor 3, Heat to 80°C, add RMIM lipase with 8% total weight of lard and fatty acid, react at constant temperature for 40min, then cool down to 37°C at a rate of 5°C / min, keep constant temperature for 60min, then turn on the vacuum, remove lipase by centrifugal filtration, The product was molecularly distilled (6mmHg, 150°C) to remove free fatty acids. The product goes into 4. HPLC-ELSD analysis of OPO content, GC analysis of fatty acid composition, as follows:

[0051]

[0052] The content of OPO increased from 13.75% to 35.48% of the raw material of pig fat oil, and the change rate of palmitic acid content in the composition of sn-2 fatty acid was 3.9%.

[0053] Step 5 Add t...

Embodiment 3

[0055] Step 1-2 is basically the same as embodiment 1

[0056] Step 3-4 Put the lard decholesterolated in the above step 1 and the fatty acid refined in the step 2 into the tank 1 at a ratio of 0.8:1, heat to 42°C, dissolve and mix well, and then pump into the above reactor 3, Heat to 80°C, add RMIM lipase with 3% total weight of lard and fatty acid, react at constant temperature for 40min, then cool down to 37°C at a rate of 3°C / min, keep constant temperature for 60min, then turn on the vacuum, and remove lipase by centrifugal filtration. The product was molecularly distilled (6mmHg, 150°C) to remove free fatty acids. The product goes into 4. OPO content was analyzed by HPLC-ELSD, and fatty acid composition was analyzed by GC. OPO content increased from 13.75% to 45.48% of the lard oil raw material, and the change rate of palmitic acid content in the sn-2 fatty acid composition was 4.3%.

[0057] Step 5 Add the triglyceride obtained above into a container with a stirring de...

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Abstract

The invention discloses a method for producing 1,3-dioleoyl-2-palmitoyl triglyceride. The invention belongs to the field of human milk substitutes and particularly relates to a method for producing triglyceride, which comprises: 1, removing cholesterol from lard by an ethanol extraction process till the cholesterol content reduces to below 500mg / kg; 2, reacting the lard from which cholesterol is removed with refined fatty acid in the presence of 1-position and 3-position selective lipase serving as a catalyst; 3, filtering to remove lipase from the mixed reactants; 4, removing the fatty acid from the product, and obtaining triglyceride; and 5, crystallizing to refine triglyceride with high OPO content. In the invention, a cholesterol removal process is introduced for the lard raw materialused in preparation, the cholesterol removal rate reaches over 92 percent, and the cholesterol content in the lard raw material from which cholesterol is removed is lowered to less than 500mg / kg. Theaddition of the refining process improves the OPO content in the triglyceride reaches over 53 percent. The difference between the palmitic acid content before reaction in sn-2 fatty acid and the palmitic acid content after the reaction in the sn-2 fatty acid is less than 10 percent.

Description

technical field [0001] The invention belongs to the field of human milk substitutes, in particular to a method for producing triglycerides. Background technique [0002] Triglycerides are important products that have been widely used in the food industry. Some triglycerides have important nutritional value, such as 1,3-dioleic acid-2-palmitic acid triglyceride (hereinafter referred to as OPO), which is a commercialized human milk fat substitute product such as betapol45 TM and Infat TM The Ministry of Health announced on No. 13 in 2008 that it is clear that OPO can be added to infant dairy products as a nutritional fortifier. [0003] The positional distribution of fatty acids in triglycerides has a significant impact on the absorption of fatty acids and minerals (such as calcium). Freeman et al. (J.Dairy Sci., 1965, p.853) reported that most of the palmitic acid in human milk fat is distributed in the sn-2 position, while stearic acid and oleic acid are distributed in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11C3/08C11B3/00C11B3/12C12P7/64A23D9/02
Inventor 邓利叶贤春李新倩王芳谭天伟
Owner BEIJING UNIV OF CHEM TECH
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