185 results about "Milk lipid" patented technology

The invention belongs to the field of fermentation, and particularly relates to a complex microbial inoculant, original cheese, processed cheese with a blood sugar reducing function and preparation and application of the complex microbial inoculant. Wherein the processed cheese is prepared by the method comprising the following steps: mixing the components including raw cheese, sugarcane juice, corn oil, casein, concentrated milk protein, blueberry powder, mono / diglycerol fatty acid ester, sodium citrate, sodium tripolyphosphate, sodium hexametaphosphate, salt guar gum, potassium sorbate and water, and then emulsifying to obtain the processed cheese, the sugar cane juice, the corn oil, the casein, the concentrated milk protein, the blueberry powder, the mono / diglycerol fatty acid ester, the sodium citrate, the sodium tripolyphosphate, the sodium hexametaphosphate, the salt guar gum, the potassium sorbate and the water, the original cheese is prepared from a complex microbial inoculant, and the complex microbial inoculant comprises lactococcus lactis bacterial powder, lactococcus lactis subsp. Lactis bacterial powder, lactobacillus delbrueckii subsp. Lactis bacterial powder, lactobacillus plantarum bacterial powder and lactobacillus paracasei bacterial powder. The processed cheese provided by the invention has a better function of controlling blood sugar, and meanwhile, the processed cheese is fine and uniform in texture and has good smearing performance.

The invention relates to the technical field of foods, and provides a micron-sized milk-fat-ball-like food colloid and a preparation method of the micron-sized milk-fat-ball-like food colloid. According to the preparation method disclosed by the invention, aloe vera, edible oil such as wheat germ oil and water are used as raw materials to prepare the micron-sized creamy-ball-like food colloid, the limitation of components of dairy products is completely eliminated, and the prepared creamy-ball-like food colloid presents a creamy-ball-like structure of which the particle size is greater than that of milk under a 200-500-time light microscope, so that the stability of the food colloid is greatly improved. The surface of the ball is negatively charged like a cream ball. The food colloid prepared by the preparation method provided by the invention has cold and hot stability from-80 DEG C to 121 DEG C, and the shelf life exceeds 12 months. The prepared food colloid with the milk fat ball-like structure is large in development potential, milk components do not need to be simulated, proper hydrophilic and hydrophobic components can be added into a water phase and an oil phase, and the food colloid can be used as a transmission system of bioactive components and can be industrially produced.

The present technical solution discloses a method for producing fermented fish sausages and fermented fish sausages that improve the distinctive flavor of the sausage. The present invention adds 5-pyridoxal phosphate, which is a cofactor that catalyzes the transaminase reaction and can catalyze the leucine in the fish protein to produce α-ketoisocaproic acid, thereby increasing the content of 3-methylbutyraldehyde; by adding a composite fermentation agent, Lactococcus lactis subspecies cremoris contains transaminase, which promotes the catabolism of leucine and isoleucine to produce 3-methylbutyraldehyde and 2-methylbutyraldehyde; Lactobacillus sakei contains α-ketoacid decarboxylase, which promotes the catabolism of leucine to produce 3-methylbutyraldehyde. The present technical solution can improve the fermentation efficiency of naturally fermented sausages and regulate the flavor of the fermented sausages, so that the fermented fish sausages have a rich caramelized aroma, fruity flavor, and bacon flavor, and a delicate, smooth, and elastic taste.

The invention discloses an FABP3 gene SNPs marker for detecting sheep milk production traits and application of the FABP3 gene SNPs marker, and belongs to the technical field of biology. According to the invention, lactating sheep is taken as a research object, Sanger and other technologies are applied to detect a nucleotide sequence variation site of an FABP3 gene, a general linear mixing effect model is constructed, the correlation between nucleotide sequence variation and sheep lactation traits is researched, and a molecular genetic marker for regulating and controlling the sheep lactation traits is excavated; on the SNP1 site, the cream-fat percentages of AG and GG genotype individuals are respectively 0.99% and 1.01% higher than those of AA type individuals. On the site SNP2, the milk fat rates of TC and TT genotype individuals are 1.18% and 1.23% higher than those of CC type individuals respectively, and the daily lactation volumes of the TC and TT genotype individuals are 1.97% and 1.27% higher than those of the CC type individuals respectively.

The invention discloses a preparation method of a low-mineralization whole casein recombinant micelle and a stable emulsion thereof, and belongs to the technical field of dairy product processing. The preparation method comprises the following steps: aiming at animal latex bundle-state casein ingredients, carrying out decalcification treatment by adopting ion exchange resin to obtain decalcified micelle-state casein; and regulating and controlling recombination environments such as casein concentration, ion species and addition concentration by regulating and controlling recombination modes such as ion addition sequence, addition batch, addition time interval, recombination pH and temperature, and further screening the decalcification rate of the micelle-state casein, thereby preparing the low-mineralization full-casein recombinant micelle. And the mineralization degree and the micelle structure are close to those of human casein micelles. Afterwards, anhydrous milk fat is added into the whole casein recombinant micelle solution, and emulsion with stable low-mineralization whole casein recombinant micelles is prepared; the improvement of protein and lipid digestibility and calcium bioavailability is realized, the fatty acid content of calcium soap is reduced, and the full utilization of animal milk casein is realized.

The invention discloses an SNP molecular marker for increasing the milk yield of Holstein cattle and application of the SNP molecular marker, the SNP molecular marker is located at a mutation site of a Holstein cattle genome Leptin gene, the gene number of the Leptin gene in an NCBI database is NC037333.1, the SNP molecular marker is located at the 1761th basic group of a cDNA sequence of the Leptin gene, and the mutation basic group is A or G. When the basic group of the SNP molecular marker is A, the genotype is AA or AG, and the milk fat rate and the milk protein rate of Holstein cow milk production are high; when the basic group of the SNP molecular marker is G, the genotype is GG, the milk yield of Holstein cattle in 305 days is high, and the milk quality is not obviously reduced. The marker is applied to genetic improvement of dairy cows, and the frequency of dominant alleles can be improved generation by generation by selecting individuals carrying the dominant alleles, so that the lactation performance of the dairy cows is effectively improved, the breeding efficiency is optimized, and the competitive advantage in the field of dairy cow breeding is enhanced.

The invention discloses a nanopore filtering and separating medium as well as a preparation method and application thereof, a polymer-based nanopore medium with accurate pore size distribution is constructed through a template-induced self-assembly technology, and efficient interception of milk fat components and selective permeation of whey protein are realized in combination with a surface functionalization modification strategy. Based on a size screening and electrostatic repulsion synergistic mechanism, milk fat enrichment and milk protein purification are synchronously completed under mild operation conditions, and energy consumption and component loss in a traditional process are remarkably reduced. And the long-term operation stability is maintained by regulating and controlling the structure parameters and surface chemical characteristics of the nano-pore channel. The technology provides an innovative solution for developing high-purity buffalo milk fat products and defatted buffalo milk products.

The invention provides a DGAT1 gene SNPs marker for detecting sheep milk production traits and application thereof.Nucleotide sequence variation sites of the DGAT1 gene are detected with the first-filial generation of Dongfudrisheng sheep male * Hu sheep female as an object, a general linear mixing effect model is constructed, the correlation between nucleotide sequence variation and sheep milk production traits is researched, and the DGAT1 gene SNPs marker for detecting the sheep milk production traits is obtained. And digging a molecular genetic marker for regulating and controlling the lactation character of the sheep. Three SNPs (single nucleotide polymorphisms) sites are found in total; wherein two variation sites in the first intron are named as c.191 + 411 Cgt, and the second intron is named as c.191 + 411 Cgt; t (SNP1) and c.192-440 Cgt, and c. 292-440 Cgt; t (SNP2) and a variation site in the 17th exon are named as c.1461 Cgt; t (SNP3). For the SNP1 site, the average daily milk yield of the CT genotype ewe is increased by 8.2% and 17.04% compared with that of the CC genotype ewe and that of the TT genotype ewe respectively; for the SNP3 site, the milk fat percentage of the CC genotype ewe is improved by 0.314% compared with that of the CT genotype ewe. A new molecular marker can be provided for selection and improvement of lactation traits of the milk sheep, and the molecular marker has important application prospects in sheep screening or breeding.

The present invention relates to the technical field of raw materials for toiletries, and specifically discloses a composite extract for preventing hair loss and controlling oil, a preparation method thereof, and an application thereof. The preparation method of the composite extract for preventing hair loss and controlling oil comprises the following steps: step 1), putting the composite plant raw material into a solvent, heating and extracting, and obtaining a pre-extract solution; step 2), cooling the pre-extract solution to ≤36°C, and then inoculating the composite bacteria into the pre-extract solution for fermentation to obtain a fermentation solution; step 3), sterilizing and concentrating to obtain a composite extract for preventing hair loss and controlling oil; the composite plant raw material is a compound of ginseng root, Platycladus orientalis, Polygonum multiflorum, Ligustrum lucidum, Morinda officinalis, Eucommia ulmoides, Milk thistle, and Taxus cuspidatus; the composite bacteria is a compound of Jardine Seberlindner's yeast, Phanerochaete chrysosporium, Hansen's yeast with grape juice, and Lactococcus lactis subspecies creamy. The present invention has the advantage of further improving the anti-hair loss effect.

The present invention discloses a targeted knockout bovine FGFR1 The application of the sgRNA of the gene in constructing a bovine mammary epithelial cell line, wherein the sgRNA nucleotide sequence is shown in SEQ ID NO: 1. The mammary epithelial cell line obtained by constructing the sgRNA of the present invention through CRISPR / Cas9 technology, FGFR1 Protein expression was significantly downregulated, and knockout FGFR1 The gene expression had no significant effect on the viability and proliferation of mammary epithelial cells. PCNA 、 Cyclin D1 、 Cyclin E1 There was no significant change in the mRNA expression of the genes related to milk protein synthesis (such as CSN1S1 、 CSN2 、 CSN3 、 EFL5 ), lactose synthesis-related genes ( GLUT1 and HKⅡ ) and milk fat synthesis-related genes ( SCD‑1 ) were significantly decreased, providing a basis for in-depth analysis FGFR1 It provides key tools for the regulatory mechanisms of mammary epithelial cell proliferation, lactation synthesis and other biological behaviors.

The invention belongs to the field of functional feeds, and discloses a preparation method of a coarse cereal feed for sheep, which comprises the following steps: crushing one or two coarse cereals of buckwheat, broom corn millet, millet and sorghum, and mixing the crushed coarse cereals with raw materials such as enzymolysis soybean meal and alfalfa meal; adding a compound bacteria solution containing lactobacillus plantarum, lactobacillus acidophilus and saccharomyces boulardii for anaerobic fermentation; the nano zinc oxide and the yucca extract are further strengthened to improve the oxidation resistance and ammonia reduction capacity of the feed; and finally, granulating and spraying a sodium alginate-chitosan coating. Through coarse cereal combination optimization, soybean meal enzymolysis pretreatment and composite flora fermentation synergistic effect, the feed protein utilization rate, the fiber degradation rate and the animal production performance are remarkably improved. Experiments show that the milk fat content of the saanen milk goats fed with the feed is increased by 15-23%, the daily gain of the Alba cashmere goats is increased by 25-35%, the feed conversion ratio is optimized to 11.2-12.3: 1, and compared with the prior art, the feed has outstanding nutrition balance and application pertinence.

The invention discloses application of thiamine in preparation of a product for treating subclinical ketosis of dairy cows, and belongs to the field of biological medicines. Tests prove that compared with dairy cows suffering from subclinical ketosis (SCK), by adding thiamine, the content of beta-hydroxybutyric acid (BHBA), the number of bacteria in milk and the number of somatic cells can be remarkably reduced, and the milk yield, the milk fat content, the acetic acid content and the A / P ratio are remarkably increased; the microbial diversity of rumen and excrement of the dairy cow can be adjusted; a metabonomics result shows that metabolite up-regulated after the thiamine is added mainly focuses on pyruvic acid metabolism, glycolysis or gluconeogenesis, tyrosine metabolism, glycerophospholipid metabolism and biosynthetic pathways of phenylalanine, tyrosine and tryptophan. The conclusion is that when thiamine is added into the SCK dairy cow, synthesis and catabolism of BHBA of an organism can be effectively regulated and controlled by adjusting the content of succinyl-coenzyme A, then subclinical ketosis is relieved, rumen fermentation is promoted, and therefore the milk yield and the milk quality are improved.

A starter culture for the fermentation of a plant-based milk alternative is provided, which is particularly suitable for the production of a plant-based yogurt alternative. The starter culture comprises lactic acid bacteria isolated from legume plant material, especially pea plant material. Preferably, the starter culture comprises lactic acid bacteria of the species Lactobacillus rhamnosus and / or Lactococcus lactis subsp. cremoris.

The invention discloses a tricholoma matsutake milk tablet for preventing tumors and a preparation method thereof, and belongs to the technical field of medicine and health food. The tablet comprises dry milk powder, dry tricholoma matsutake extract powder, dry fructus broussonetiae powder and dry garlic powder. The tricholoma matsutake extract dry powder is prepared through the steps of enzymolysis pretreatment, ultrasonic-assisted extraction, decoloration purification and the like. The dry garlic powder is prepared by enzymolysis pretreatment, spray drying, microencapsulation treatment and coating of a protective layer. By optimizing the component proportion and innovating the preparation process, the problems of low bioavailability, insufficient synergistic effect, gastric mucosa irritation and the like of natural active components are solved. High-quality protein in the dry milk powder and the tricholoma matsutake polysaccharide form a nanoscale complex, so that the bioavailability of the polysaccharide is improved; the multi-layer protection mechanism of the dried garlic powder can retain the activity of allicin and reduce the stimulation of gastric mucosa; the fructus broussonetiae component and the tricholoma matsutake polysaccharide synergistically enhance immune response and relieve eye discomfort possibly caused by allicin. Milk lipid is used as a natural adhesive, so that the safety and taste of the tablet are improved.

The invention relates to the technical field of dairy product processing, in particular to a double-fat double-protein synergistic dairy product and a preparation method thereof. According to the preparation method, a ''whey protein (WP)-casein phosphopeptide (CPP)'' double-protein system and a ''milk fat (MF)-medium chain triglyceride (MCT)'' double-fat system are constructed, and a four-step synergistic process of ''pretreatment, composite emulsification, gradient assembly and low-temperature curing'' is adopted, so that stable combination of a chemical interface of protein and fat is realized, high-temperature stability breakthrough is realized, and the preparation method has the advantages of high-temperature stability, high-temperature stability and high-temperature stability. Meanwhile, high-pressure processing tolerance is improved, fat globule flocculation caused by high pressure is avoided, and long-term storage stability, texture and taste are guaranteed.

The present invention provides a method of feeding a ruminant animal, preferably a pregnant ruminant animal, using a phytogenic composition. By feeding the composition pre-partum, the colostrum yield may be increased and colostrum quality is improved. Moreover, milk yield and milk fat percentage may be increased during lactation phase of the ruminant animal.

The invention discloses a nutritional composition for short-time internal inflammation balance after medium-high-strength resistance training and application of the nutritional composition, and belongs to the technical field of food processing. The composition comprises an inflammation control component composed of a plant inflammation control component and a milk source inflammation control component, the inflammation control component is composed of a plant source component and a milk source component and specifically comprises omega-3 unsaturated fatty acid, curcumin, iron unsaturated lactoferrin and a milk fat globule membrane according to a specific weight ratio, the weight ratio of EPA to DHA in the omega-3 unsaturated fatty acid is not lower than 1.5: 1, and the weight ratio of the curcumin to the iron unsaturated lactoferrin to the milk fat globule membrane is not lower than 1.5: 1. The iron saturation of the iron unsaturated lactoferrin is less than or equal to 30%, and the phospholipid accounts for 4-10% of the milk fat ball membrane. Experimental results show that the composition can effectively regulate and control the level of inflammatory factors within 6 hours after training, and accurate regulation and control of inflammatory response within an inflammatory regulation window period within 6 hours after training are achieved. The invention also relates to application of the composition in preparation of functional food and dairy products.

The utility model relates to the technical field of whey separation, in particular to a fat and whey separator in milk, which is characterized in that when the fat and whey separator is used, milk is stirred by a stirring component, milk fat particles are more uniformly distributed in the milk by stirring, subsequent separation is facilitated, and then a liquid conveying component is started; therefore, the stirred milk is filtered through one station of the double-station filtering assembly and cleaned through the other station, continuous separation of fat and whey of the milk is kept while filtering and cleaning are achieved, and the separation efficiency is improved; comprising a bottom plate and a disc type centrifugal machine, the disc type centrifugal machine is installed at the top end of the bottom plate, the device further comprises a stirring assembly, a liquid conveying assembly and a double-station filtering assembly, the liquid conveying assembly and the stirring assembly are arranged at the top end of the bottom plate, and the input end of the liquid conveying assembly communicates with the output end of the stirring assembly; the output end of the liquid conveying assembly communicates with the input end of the double-station filtering assembly, and the output end of the double-station filtering assembly communicates with the input end of the disc type centrifugal machine.

The invention belongs to the technical field of intestinal health care, and relates to a composition containing a milk fat globular membrane and application of the composition. The composition comprises the following components in parts by weight: 0.1 to 0.4 part of a milk fat ball membrane, 0.4 to 2.4 parts of fructo-oligosaccharide, 0.1 to 1 part of xylooligosaccharide, 0.1 to 0.5 part of stachyose and 0.1 to 0.5 part of metabiotics. The composition can increase beneficial bacteria, inhibit growth of harmful bacteria and increase intestinal peristalsis; the intestinal permeability is regulated; meanwhile, the level of tight junction protein and mucoprotein in the intestinal tract is adjusted, and the intestinal tract barrier is enhanced; the intestinal inflammation level can also be regulated. The composition improves intestinal health through multiple targets, and has a remarkable effect on improving intestinal problems such as constipation and inflammatory bowel diseases; meanwhile, depression and anxiety mood caused by intestinal problems can be relieved, the life quality is improved, and the traditional Chinese medicine composition is more beneficial to overall health of organisms and can be taken for a long time.

The invention relates to the field of molecular biology, and discloses a haplotype marker for dairy cow milk production butter-fat percentage character breeding and application of the haplotype marker, the haplotype marker is composed of three SNP sites in a strong linkage imbalance state, and the three SNP sites are located in a Block1 haplotype block region of a 10 # chromosome NC037337.1 of a dairy cow ARS-UCD1.2 genome; the three SNP sites are as follows: SNP1: g.59680573 A > C, SNP2: g.59743173 G > C, and SNP3: g.59743414 C > T; the dominant haplotype of the haplotype marker is H2, and the allele combination of the haplotype marker is SNP1-A, SNP2-G and SNP3-C; the homozygous H2H2 of the dominant haplotype H2 is significantly related to the high milk fat percentage character of milk production of dairy cows. The bottleneck of the traditional technology is solved, and the breeding accuracy is remarkably improved; the screening detection technical scheme is stable and reliable and has a wide application range; the milk production quality of groups is improved, and the industrial economic value is enhanced.

The present application relates to a kind of fat powder for reducing weaning piglet dead rate and its preparation method and application, and the composite lipid in the preparation raw material of fat powder includes 1,3-di-oleic acid-2-palmitic acid triglyceride, 24 degrees palm oil, rice bran oil, coconut oil, linseed oil, caprylic triglyceride, capric acid triglyceride, alpha-lauric acid monoglyceride and tributyrin.The present application develops the fat acid composition close to pig milk fat structure, let piglet continue lactation period in weaning period to enable approach mainly with lipid, meet the compound energy demand of fast, medium and slow, avoid falling into energy potential difference;And the specific combination of medium and short chain fatty acids, both can improve the energy efficiency of weaning piglet, also have bacteriostatic disease characteristics, can improve the immunity of piglet, reduce the diarrhea rate.This composite lipid overcomes the problem such as piglet energy deficiency and indigestion caused by single raw material oil due to unsuitable lipid structure, can significantly reduce weaning stress, reduce the diarrhea rate and dead rate.

This invention discloses a *Lactococcus faecium* strain, YS-IM01. This strain can efficiently hydrolyze sheep colostrum proteins and specifically release a large amount of leucine. This invention also provides a polypeptide composition comprising multiple active peptides with potential for muscle synthesis, immunomodulation, and antioxidant activity. This invention further provides a fermented sheep colostrum product obtained by fermenting sheep colostrum with the aforementioned *Lactococcus faecium*. This invention also provides a method for preparing the fermented sheep colostrum product, comprising steps including fermentation, low-temperature vacuum concentration, and vacuum freeze-drying. Simultaneously, this invention also provides a method for preparing a drug using *Lactococcus faecium* and the polypeptide composition to prevent and / or improve age-related muscle loss.

The application discloses a kind of fermented whipped cream and method prepared by using two-phase oil gel, belong to fermentation milk processing technical field.The application is prepared oil phase gel with enzymolysis coconut oil, rapeseed protein as raw material, and prepared water phase gel with selenized carrageenan and oxidized konjac glucomannan, then two-phase oil gel is formed by processing;Cream raw material is pre-fermented by saccharomyces cerevisiae, and cream microspheres are obtained by high-pressure homogenization, then fermented whipped cream is obtained by mixing two-phase oil gel and compound lactic acid bacteria.The application constructs stable crosslinking network by cysteine, gives two-phase oil gel good elasticity and stability, cream microspheres can avoid sedimentation and aggregation, and compound lactic acid bacteria fermentation not only promotes the system to form uniform gel network, but also retains characteristic flavor.The fermented whipped cream prepared by the application has uniform texture and rich and lasting flavor, has nutrition and taste advantage, can effectively solve the problem of loose structure and easy flavor loss of traditional fermented whipped cream, and has good industrial application prospect and market value.

The invention discloses a construction method of a cheese product with high mouth melting property. The construction method comprises the following steps: determining a basic formula model of the cheese product; starch and dextrin raw materials with different sources are selected, cheese prepared from whole milk fat is used as a benchmarking product, and products with different mouth melting properties are prepared; constructing a sensory evaluation method; establishing the association between the raw materials and the mouth melting property of the cheese from the gel melting time and the molecular weight interval; determining the texture, viscosity, low-field nuclear magnetism, SFC and rheological result indexes of different cheese samples, and establishing the correlation between the mouth melting property of the cheese and the related detection indexes; comparing sensory evaluation values; the directions of raw materials need to be selected for obtaining products with different mouth melting properties through derivation, and cheese products with high mouth melting properties are obtained. By means of the construction method, in the formula design stage, the product formula can be designed according to the molecular weight condition, and the mouth feel of the product is ensured; and meanwhile, objective and quantitative mouth melting property detection and evaluation can be carried out on the mouth melting property of the finished cheese.

The present application belongs to the technical field of shrimp seafood food processing, and particularly relates to a kind of astaxanthin-containing shrimp food and a preparation method thereof.The composition includes the following components: astaxanthin embedding, sea white shrimp meat, rhizoma dioscoreae nigrae, carotene, sodium citrate, water, coconut milk, low-ester pectin, calcium chloride, cassava starch, trehalose, sodium alginate, chitosan, tea polyphenol, vitamin C and glycerol.The present application utilizes the complementary cooperation of fat-soluble astaxanthin-carotene and water-soluble tea polyphenol-vitamin C, the metal chelation of sodium citrate, and the multiple protection of coconut milk lipid membrane, trehalose and pectin-starch network in reducing water activity and oxygen permeation, to realize the synergistic innovation of nutritional stability, texture optimization, sensory innovation and safe preservation.

The present invention relates to bovine milk fat comprising, based on total fatty acid content, 8.9-29.0 wt % linoleic acid (C18:2 cis, 9,12), 0.9-2.4 wt % alpha-linolenic acid (C18:3 cis 9,12,15), and 3-5 wt % butyric acid (C4:0). The present invention further relates to a bovine milk comprising said milk fat and to a method for producing said milk by feeding a lactating bovine mammal with a rumen-protected linoleic acid source and a rumen-protected alpha-linolenic acid source, followed by milking the lactating bovine animal.

The invention discloses a molecular marker for detecting the milk production character of cattle and an identification method, and belongs to the technical field of biology. The SNP A provided by the invention is g.8044024C / T, the SNP B provided by the invention is g.8081101 A / G, and the SNP C provided by the invention is g.8081205C / G. The molecular marker g.8044024C / T, g.8081101 A / G or / and g.8081205C / G is associated with the milk yield, the milk fat amount and the milk protein amount at a remarkable or extremely remarkable level (P = 0.02-2.00 E-16). By utilizing the SNP provided by the invention, early screening can be realized in the dairy cow breeding process, the breeding cost can be reduced, and the SNP has an important application prospect in dairy cow breeding.

The invention relates to the technical field of microorganisms, and in particular relates to a prevotella bruceli B14 capable of improving the milk yield and the milk-fat percentage of ruminants and application of the prevotella bruceli B14. The invention discloses a strain of prevotella bruceli B14, and the Latin name of the prevotella bruceli B14 is prevotella bruceli B14. The preservation number of the strain is CCTCC NO: M 20242730. The prevotella bruceli B14 provided by the invention can increase the milk yield and the milk fat percentage of ruminants by increasing the concentration of total volatile fatty acid, acetic acid, propionic acid and valeric acid in the rumen of the ruminants. In addition, the compound also has the function of synthesizing nicotinic acid, and can activate an mTORC1 signal channel in the mammary gland of the ruminant in a GPR109A-dependent manner and promote the expression of lipid synthesis genes so as to promote milk fat synthesis. In addition, the prevotella buchneri B14 also has the function of reducing the abundance of methanogenic archaea in rumen, so that the prevotella buchneri B14 is expected to improve the energy utilization efficiency by reducing the methane emission of ruminants.

A fat formulation can include triacylglycerides that can be made of glycerol esterified with straight-chain saturated fatty acids, where a solid fat content as a function of temperature of the triacylglycerides differs from a solid fat content of butterfat by at most 0.1 at any temperature between 0° C. and 40° C. A fat formulation can include triglycerides that include straight-chain, saturated fatty acids selected from a distribution of fatty acids with between 4 and 24 carbon atoms, where the distribution comprises a non-monotonic change in relative fatty acid content between the fatty acids with between 4 and 24 carbon atoms.

Fat blend comprising (i) 20-65 wt % of a vegetable lipid source and (ii) 35-80 wt % of a milk fat composition comprising at least one bovine milk fat fraction, wherein the milk fat composition comprises, relative to total fatty acid moieties, 37-48 mol % of long chain saturated fatty acid moieties with 12-18 carbon atoms (LCSFA C12:0-C18:0) and wherein 28-43 mol % of all fatty acid moieties positioned on the sn-1,3 position of the glycerol backbone in said milk fat composition are long chain saturated fatty acid moieties with 12-18 carbon atoms (LCSFA C12:0-C18:0).