Application of rumen bypass melatonin in improvement of ruminant rumen microbial flora structure and improvement of milk quality
A technology for rumen microorganisms and ruminants, applied in applications, animal feeds, drug combinations, etc., can solve the problem of no significant effect on milk melatonin content, and achieve the goal of improving milk quality, reducing lactose content, and improving protein utilization. Effect
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[0076] Example 1 Verification of the effect of rumen melatonin in reducing somatic cells in milk and improving milk quality
[0077] 1. Feeding of melatonin over rumen
[0078] Thirty-five cows with higher somatic cells (somatic cells ranging from 300,000 / mL to 1,000,000 / mL) were selected and divided into 3 groups. There was no significant difference in the body weight of cows within and between groups, with an average body weight of 600 kg. Fifteen animals in the rumen melatonin-containing 40mg group (experimental group 1, that is, each cow was fed rumen melatonin, in which the effective dose of melatonin was 40mg), the 80mg group 15 cows (experimental group 2, that is, each cow was fed rumen melatonin, wherein the effective dose of melatonin was 80 mg), and 5 cows in the control group (not fed rumen melatonin). They were fed rumen melatonin for 21 consecutive days, and milk samples were collected every two days. Every 7 days, 5 cows in the experimental group were randomly c...
Example Embodiment
[0090] Example 2 Verification of the effect of melatonin in improving the microflora in the rumen
[0091] 1. Experimental method
[0092] (1) 0.6μg / kg~0.13mg / kg of transrumen melatonin (the values here refer to the effective dose of melatonin) were injected into the rumen fistulas of 3 cows with similar age and physiological conditions, and 6 Take it out after an hour, and record the time at which melatonin was put in as 0 o'clock. Rumen fluid was taken at 0, 6 and 6 hours after the melatonin preparation was taken (ie, 12 o'clock).
[0093] (2) The rumen microflora DNA was extracted from the rumen fluid taken at different time points, and then a total of 1,351,116 raw reads were obtained by 16S rRNA sequencing. After removing chimeras and short sequences, 1,334,733 valid reads were obtained, with an average of 1,334,733 valid reads per sample. 1,334,733 reads, ranging from 25,820 to 316,522.
[0094] 2. Experimental results
[0095] (1) OUT is divided at the level of se...
Example Embodiment
[0097] Example 3 Effects of melatonin on related hormones in cow blood and milk
[0098] 1. Over-rumen melatonin feeding
[0099] The feeding method was the same as that in Example 1. The blood samples were collected at the same time as the milk samples, and blood was collected from the tail vein of the cows.
[0100] 2. Determination of related hormone concentrations in serum and milk
[0101] The types of hormones determined in cow serum were: MT, TNF-α, IL-6, IL-10, and cortisol. The types of hormones determined in milk were: MT, TNF-α, IL-10. The assay method is enzyme-linked immunoassay (ELISA).
[0102] 3. Experimental results
[0103] (1) Increase the concentration of melatonin in serum. pass Figure 20 , the melatonin content in the serum of the 80mg group fed rumen melatonin for 7 days was significantly higher than that of the control group (P Figure 21 , the melatonin content in the serum of the 40mg and 80mg groups fed rumen melatonin for 14 days was significa...
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