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Food grade carrier of lactic galactococcus

A Lactococcus lactis, food-grade technology, applied in the direction of using vectors to introduce foreign genetic material, bacteria, recombinant DNA technology, etc.

Inactive Publication Date: 2005-08-03
ICDC CHINA CDC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Select thyA gene as a selection marker to construct a food-grade carrier system for Lactococcus lactis, but there is no report in this regard at home and abroad

Method used

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  • Food grade carrier of lactic galactococcus
  • Food grade carrier of lactic galactococcus
  • Food grade carrier of lactic galactococcus

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Embodiment Construction

[0052] In recent years, the development of genetically engineered vaccines has achieved rapid development, but there are still some problems, that is, the expression receptors of the recombinant bacterial protective antigens are mainly live and attenuated pathogens, and their immune effects depend on the receptors used. The reproductive ability, invasion ability, survival time and expression level of corresponding antigen genes of the bacteria, and the immune protection of the vaccine is often proportional to the residual virulence of the strain. Therefore, people have been trying to find strains that can express foreign antigen genes well and are safe. The ideal direct edible microbial strain should have: 1. It will not cause disease to humans and animals, and it will not produce hybrids with pathogenic microorganisms; 2. It is easy to reproduce in vitro and in vivo, and the reproduction speed is fast in vitro; 3. Low pH 4. It can produce lactic acid and hydrogen peroxide and...

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Abstract

The present invention provides a kind of food grade carrier of lactic galactococcus preserved in the preservation number of CGMCC No. 1263. That is constituted food grade carrier pSH91 containing lactic galactococcus MG1363 thyA gene selecting label, lactic galactococcus cream subspecies Wg2 recessive plasmid pWV01 replicon and pUC18 plasmid polyclonal site. The present invention lays foundation for the cloning and expression of foreign gene in lactic galactococcus.

Description

1. Technical field [0001] The present invention relates to a lactic acid bacteria carrier, in particular to a food-grade carrier pSH91 of a Lactococcus lactis ΔthyA mutant strain. 2. Background technology [0002] Lactic acid bacteria (LAB) is a general term for a class of bacteria that can utilize fermentable sugars to produce a large amount of lactic acid, including more than twenty genera such as Lactococcus, Lactobacillus, Bifidobacterium, and Enterococcus. Lactic acid bacteria are the most widely used important industrial strains in food, and have long been used by humans to make pickles, soy sauce, and produce bread, cheese, yogurt and other dairy products; lactic acid bacteria also exist in the intestinal tract of animals, including humans, so it is recognized Food-grade microorganisms (generally regarded as safe, GRAS). A growing number of studies have shown that certain strains of lactic acid bacteria have important promoting effects on human health, including biol...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/74
Inventor 熊衍文徐建国
Owner ICDC CHINA CDC
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