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Food grade carrier of lactic galactococcus

Lactococcus lactis, food-grade technology, applied in the field of lactic acid bacteria carriers

Inactive Publication Date: 2007-11-21
ICDC CHINA CDC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Select thyA gene as a selection marker to construct a food-grade carrier system for Lactococcus lactis, but there is no report in this regard at home and abroad

Method used

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  • Food grade carrier of lactic galactococcus
  • Food grade carrier of lactic galactococcus
  • Food grade carrier of lactic galactococcus

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Embodiment Construction

[0052] In recent years, the development of genetic engineering vaccines has achieved rapid development, but there are still some problems, that is, the expression receptors of recombinant bacterial protective antigens are mainly live, attenuated pathogenic bacteria, and their immune effect depends on the use of the receptors. The reproductive ability, invasion ability, survival time and the expression level of the corresponding antigen gene of the somatic bacteria, the immune protection of the vaccine is often proportional to the residual virulence of the strain. Therefore, people have been trying to find a strain that can express exogenous antigen genes well and is safe. The ideal microbial strains that can be directly eaten should have: 1. It will not cause diseases to humans and animals, and will not produce hybrids with pathogenic microorganisms; 2. It is easy to reproduce in vivo and in vitro, and the reproduction speed is fast in vitro; 3. At low pH 4. It can produce lac...

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Abstract

The present invention provides a kind of food grade carrier of lactic galactococcus preserved in the preservation number of CGMCC No. 1263. That is constituted food grade carrier pSH91 containing lactic galactococcus MG1363 thyA gene selecting label, lactic galactococcus cream subspecies Wg2 recessive plasmid pWV01 replicon and pUC18 plasmid polyclonal site. The present invention lays foundation for the cloning and expression of foreign gene in lactic galactococcus.

Description

1. Technical field [0001] The invention relates to a lactic acid bacteria carrier, in particular to a food-grade carrier pSH91 of a Lactococcus lactis ΔthyA mutant strain. 2. Background technology [0002] Lactic acid bacteria (LAB) is a general term for a class of bacteria that can use fermentable sugars to produce a large amount of lactic acid. It includes more than 20 genera such as Lactococcus, Lactobacillus, Bifidobacterium, and Enterococcus. Lactic acid bacteria are the most widely used important industrial strains in food. They have long been used by humans to make kimchi, soy sauce, bread, cheese, yogurt and other milk products; lactic acid bacteria also exist in the intestines of animals including humans, so it is recognized It is a safe food-grade microorganism (generally regarded as safe, GRAS). More and more studies have shown that certain strains of lactic acid bacteria have important promotional effects on human health, including biological barriers, synthesis...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/74C12R1/01C12N1/21
Inventor 熊衍文徐建国
Owner ICDC CHINA CDC
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