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Method for producing propenamide using film technique microbiological transformation

A technology for microbial transformation and acrylamide, applied in fermentation and other directions, can solve the problems of high biological impurity content, complicated equipment maintenance and high energy consumption in acrylamide products, improve reaction efficiency and bacterial cell utilization rate, and simplify production equipment and processes. , the effect of simplifying the ion exchange step

Inactive Publication Date: 2006-01-25
TSINGHUA UNIV +1
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Problems solved by technology

The main problems in the current technology are: the process of cell immobilization is relatively complicated, which also increases equipment investment and production costs; the enzyme activity of cells after immobilization is greatly reduced, which affects the utilization of enzyme activity; It will break, produce organic and inorganic impurities, and affect the quality of the reaction solution; therefore, the hydration reaction solution needs to be refined in multiple steps to meet the quality requirements, which makes the refining process heavier, etc.
This process needs to use a high-speed centrifuge, which requires a large investment in equipment, complicated equipment maintenance, high energy consumption, and the content of biological impurities in the obtained acrylamide product is also relatively high.

Method used

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  • Method for producing propenamide using film technique microbiological transformation
  • Method for producing propenamide using film technique microbiological transformation

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Experimental program
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Embodiment 1

[0011] Preparation of bacterial resuspension:

[0012] (1) Bacteria were cultured in a 3m3 fermenter at 28°C. The main components of the medium were: glucose 20g / L; yeast extract powder 5g / L; urea 7.5g / L; sodium glutamate 1g / L ;K 2 HPO 4 0.5g / L; KH 2 PO 4 0.5g / L; MgSO 4 ·7H 2 O0.5g / L; inducer 60ppm. The pH value was adjusted to 7.5, and after culturing for 56 hours, a total of about 1.2 tons of bacterial suspension was obtained, the wet bacterial content was 120 g / L, and the enzyme activity reached 2669 U / mL.

[0013] (2) The bacterial suspension is sent to the bacterial washing tank with a pump, and cooling brine is introduced into the cooling jacket of the washing tank, so that the temperature of the bacterial liquid in the washing process is controlled at 4-10 °C.

[0014] (3) Start the circulating pump, the speed is 2900rpm, and the flow rate is 12.5m 3 / h, the bacterial re-suspension is filtered and concentrated through the hollow fiber microfiltration me...

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Abstract

The microbial conversion process of producing acrylamide by applying membrane technology includes the following steps: microbial thallus culture, preparation of heavy thallus suspension, acrylonitile hydration to produce acrylamide with free thallus as biocatalyst, and separating reaction obtained acrylamide hydrate liquid. The present invention features the microfiltering film purification and washing of thallus in fermented liquid to prepare heavy thallus suspension and the ultrafiltering film separation of acrylamide hydrate liquid from biological impurity. The production process of acrylamide has high production efficiency, high thallus utilization, low biological impurity content in hydrate product, and high quality and purity of acrylamide product.

Description

technical field [0001] The invention relates to an application method of membrane technology in the production of acrylamide by microbial transformation, and mainly includes the application of microfiltration membrane in the process of free cell washing, the application of ultrafiltration membrane in the process of free cell catalytic hydration reaction, and the application of these two parts of the process. The entire production process flow of acrylamide microbial conversion. It belongs to the field of biochemical technology. Background technique [0002] Microbial transformation method is a new generation method for industrial production of acrylamide. The technological process is divided into eight parts: fermentation, granulation, hydration, refining, concentration, crystallization, centrifugation and drying. In its hydration section, the immobilized particles are separated from the hydration liquid by a screen. The main problems existing in the current process are: ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/02
Inventor 沈忠耀孙旭东于慧敏史悦端木勉刘正光沈瑞华顾宏军薛建菲
Owner TSINGHUA UNIV
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