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Method for coupled production of cadaverine by using microbial fermentation and microbial conversion

A microbial fermentation and microbial technology, which is applied in the field of cadaverine production, can solve problems such as complex processes, and achieve the effects of simple production process, high production efficiency, and increased output

Active Publication Date: 2016-03-30
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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Problems solved by technology

Although this method does not require the purification of lysine, it requires the production and purification of lysine decarboxylase, which is a very complicated process

Method used

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  • Method for coupled production of cadaverine by using microbial fermentation and microbial conversion
  • Method for coupled production of cadaverine by using microbial fermentation and microbial conversion
  • Method for coupled production of cadaverine by using microbial fermentation and microbial conversion

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Embodiment Construction

[0026] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0027] The methods used in the present invention, unless otherwise specified, are conventional methods in the art; the reagents used in the present invention, unless otherwise specified, are commonly used reagents in the art.

[0028] The meaning of the technical terms involved in the present invention:

[0029] "Cadridine" is 1,5-pentanediamine.

[0030] "Recombinant strains" refer to non-wild-type strains, including non-wild-type strains obtained by mutation breeding, genetic engineering breeding or any other method.

[0031] "Gene knockout" refers to the knockout or mutation of the target gene, the ribosome binding site of the target gene, the promoter of the target gene or the regulatory gene of the target gene, so that the target ge...

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Abstract

The invention relates to a method for coupled production of cadaverine by using microbial fermentation and microbial conversion. By use of the gene engineering technology, lysine decarboxylase genes with different sources are cloned in Escherichia coli or corynebacterium glutamicum with high yield of lysine for secretory expression, recombinant stains are fermented, the recombinant strains synthesize lysine at the first stage, lysine decarboxylase genes are induced for secretory expression at the second stage, and the lysine is converted into the cadaverine. The method for coupled production of cadaverine by using microbial fermentation and microbial conversion provides a new way for production of the cadaverine, has enormous economic benefit and social benefit and has broad market development prospect.

Description

technical field [0001] The invention belongs to the technical field of cadaverine production, and relates to a method for producing cadaverine by coupling lysine synthesized by microbial fermentation and lysine transformed by microorganisms, and relates to the recombination of high-yield lysine capable of secreting and expressing lysine decarboxylase There are three aspects: the construction of bacteria, the fermentation of recombinant bacteria to produce lysine and the expression of lysine decarboxylase to convert lysine by recombinant bacteria. Background technique [0002] Cadaverine (Cadaverine) is a polyamine, that is, 1,5-pentanediamine (pentamethylenediamine for short), which is produced by decarboxylation of lysine in organisms, and is a biologically active nitrogen-containing compound widely present in organisms. Alkaline, but also present in spoilage as a carnitine. Cadaverine is an important raw material for the synthesis of new materials polyamide-54 (condensed ...

Claims

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Application Information

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IPC IPC(8): C12P13/00C12R1/15C12R1/19
CPCC12N9/88C12P13/001C12Y401/01018
Inventor 黎明路福平
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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