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Method for preparing ginseng rare anti-cancer saponin Compound K from composite bacteria

A technology of rare ginseng saponins and compound bacteria, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficulty in guaranteeing the safety of microorganisms, the products cannot be directly eaten, and the conversion rate needs to be improved, so as to achieve production Low cost, better flavor and taste, better nutritional value

Active Publication Date: 2017-03-15
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In summary, the current microbial transformation method to prepare rare ginseng saponin CK has problems such as the safety of microorganisms is difficult to guarantee, the process is cumbersome, the obtained product needs to be separated and purified and cannot be eaten directly, the production cycle is long, and the conversion rate needs to be improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Compound microbial transformation Rb1 is Compound-K

[0035] Candida NRRL Y-6888 and Saccharomyces NRRL Y-2388 were cultured with 4-degree wort plus 2% lactose at 30°C for 2-3 days, plant lactic acid bacteria NRRL-4496 and ATCC14917 were cultured with MRS, milk bifidum B94,, Bifidobacterium breve ATCC15700 use medium (glucose 15.85g / L, fructo-oligosaccharide 15.85g / L, tryptone 12.04g / L, beef extract 7.23g / L, yeast powder 9.63g / L, ammonium citrate 2.75g / L, K 2 HPO 4 ·3H 2 O 2.75g / L, sodium acetate 6.875g / L, Tween-801.0mL, MgSO 4 ·7H 2 O 0.2g / L, MnSO 4 ·H 2 O 0.05g / L, L-cysteine ​​hydrochloride 0.5g / L), cultured at 37°C for 2 days. Collect the cells by centrifugation, mix the cells, and dilute with 25mmol / L acetate buffer (pH6.0) to a cell concentration of 10 9 / mL, add an equal amount of 1mg / mL Rb1, shake the flask at 37°C for 5 days, add an equal amount of n-butanol for extraction, evaporate the upper n-butanol phase to dryness under reduced press...

Embodiment 2

[0036] Embodiment 2: Composite microbial solid fermented red ginseng powder produces Compound-K

[0037] Candida NRRL Y-6888 and Saccharomyces NRRL Y-2388 were cultured with 4-degree wort plus 2% lactose at 30°C for 2-3 days, plant lactic acid bacteria NRRL-4496 and ATCC14917, and Lactobacillus casei ATCC 393 were cultured with MRS , milk bifido B94, Bifidobacterium breve ATCC 15700 use medium (glucose 15.85g / L, fructooligosaccharide 15.85g / L, tryptone 12.04g / L, beef extract 7.23g / L, yeast powder 9.63g / L, ammonium citrate 2.75g / L, K 2 HPO 4 ·3H 2 O 2.75g / L, sodium acetate 6.875g / L, Tween-801.0mL, MgSO 4 ·7H 2 O 0.2g / L, MnSO 4 ·H 2 O 0.05g / L, L-cysteine ​​hydrochloride 0.5g / L), cultured at 37°C for 2 days. Obtain yeast, lactic acid bacteria (that is, type A bacteria) and bifidobacteria (that is, type B bacteria) strains, and the ratio of yeast, lactic acid bacteria and bifidobacteria is listed as 1:1:2, and the inoculation amount is 15% to inoculate red ginseng In the...

Embodiment 3

[0038] Embodiment 3: Composite microbial solid fermented red ginseng powder produces Compound-K

[0039] Candida NRRL Y-6888 and Saccharomyces NRRL Y-2388 were cultured with 4-degree wort plus 2% lactose at 30°C for 2-3 days, plant lactic acid bacteria NRRL-4496, plant lactic acid bacteria ATCC14917, Lactobacillus casei ATCC 393, Lactobacillus rhamnosus NRRL 442 was cultured with MRS, Bifidobacterium lactis and Bifidobacterium breve were cultured with medium (glucose 15.85g / L, fructo-oligosaccharide 15.85g / L, tryptone 12.04g / L, beef extract 7.23g / L , yeast powder 9.63g / L, ammonium citrate 2.75g / L, K 2 HPO 4 ·3H 2 O 2.75g / L, sodium acetate 6.875g / L, Tween-801.0mL, MgSO 4 ·7H 2 O 0.2g / L, MnSO 4 ·H 2 (00.05g / L, L-cysteine ​​hydrochloride 0.5g / L), cultivated for 2 days at 37°C. Obtain yeast, lactic acid bacteria (i.e. type A bacteria) and bifidobacteria (i.e. type B bacteria) strains, the ratio of yeast, lactic acid bacteria and bifidobacteria is listed as 1:1:2, and the in...

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PUM

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Abstract

The invention discloses a method for preparing ginseng rare anti-cancer saponin Compound K from composite bacteria, and belongs to the fields of functional food and pharmacy. According to the method, RB1 or ginseng, American ginseng and panax notoginseng serve as raw materials, 1 to 5 percent of sugar and milk powder are added, preferable saccharomycetes for producing beta-glucosaccharase, lactic acid bacteria and bifidobacterium are adopted, fermentation is conducted at 30 to 37 DEG C for 5 to 14 days, microbial transformation of the compound bacteria is conducted, and the rare ginseng containing saponin C-K is obtained. The obtained fermented ginseng powder and panax notoginseng powder have strong and attractive fragrance and good taste, and the health-care effect and the nutritional value of ginseng and panax notoginseng are greatly improved.

Description

technical field [0001] The invention relates to a method for preparing ginseng rare anticancer saponin Compound K by compound bacteria, belonging to the fields of functional food and pharmacy. Background technique [0002] Ginseng is a traditional precious medicinal material in my country, which has the functions of invigorating vitality, nourishing the spleen and lungs, promoting body fluid, calming the nerves and improving intelligence. Ginsenoside is the main active ingredient of ginseng. Scientists from all over the world have discovered that ginseng contains 128 kinds of monomeric saponins, and 40 kinds of them can be extracted by modern high-tech technology and have their clinical efficacy. Ginsenosides can be divided into 3 types, one is oleanane type pentacyclic triterpenoid saponins, and its saponin is oleanolic acid; the other two types are ginsengdiol type saponins (such as Rb1, Rb2, Rc, Rd, F2, Rg3, Rh2, etc.) and panaxatriol-type saponins (such as Re, Rg1, Rg2,...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P33/20C12R1/72C12R1/645C12R1/01C12R1/25C12R1/245
CPCC12P33/20C12P39/00
Inventor 余晓斌顾秋亚曹效成方磊
Owner JIANGNAN UNIV
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