Disease-resistant growth promoter for aquatic animals and preparation method thereof

A disease-resistant growth-promoting agent, aquatic animal technology, applied in animal feed, animal feed, application, etc., can solve problems such as residues, hidden dangers of food safety, drug resistance, etc., to achieve no drug resistance, improve immunity, quick results

Active Publication Date: 2011-11-09
BEIJING KEEPYOUNG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a disease-resistant and growth-promoting agent for aquatic animals with stable, reliable and quick effects, whic

Method used

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  • Disease-resistant growth promoter for aquatic animals and preparation method thereof
  • Disease-resistant growth promoter for aquatic animals and preparation method thereof
  • Disease-resistant growth promoter for aquatic animals and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Chinese medicine: 6 parts of dandelion, 3 parts of licorice, 3 parts of Sophora flavescens, 4 parts of three needles

[0032] Preparation:

[0033] (1) Inoculate Bacillus subtilis into a common nutrient broth medium, culture at 37℃, 180rpm shaker to produce first-grade seed liquid, when the bacterial content reaches 10 7-8 At about CFU / ml, inoculate the first-level seed liquid at 5% of the inoculum amount into the second-level seed fermenter containing nutrient broth for expansion, the culture temperature is 32℃, the rotation speed is 180rpm, when the bacterial content reaches 10 9 When CFU / ml is about, stop fermentation and reserve;

[0034] (2) Inoculate Candida utilis into the wort medium, culture at 28°C and 180rpm in a shaker to produce first-level seed liquid, when the bacterial content reaches 10 7-8 At about CFU / ml, inoculate the first-level seed liquid with the inoculum amount of 5% into the second-level seed fermenter containing nutrient broth to expand the culture, t...

Embodiment 2

[0038] Traditional Chinese medicine: 5 parts of dandelion, 3 parts of licorice, 1 part of Sophora flavescens, 2 parts of three needles, 1 part of Scutellaria baicalensis Georgi, 1 part of Artemisia annua

[0039] Preparation:

[0040] (1) Inoculate Bacillus licheniformis into a common nutrient broth medium, culture at 37℃, 180rpm shaker to produce first-grade seed liquid, when the bacterial content reaches 10 7-8 When CFU / ml is about, the first-level seed solution is inoculated into the second-level seed fermentor with the inoculum amount of 3%, and the culture temperature is 37℃ and the rotation speed is 180rpm. When the bacterial content reaches 10 9 When CFU / ml is about, stop fermentation and reserve;

[0041] (2) Inoculate Lactobacillus acidophilus into MRS medium, culture at 37℃, 180rpm shaker to produce first-grade seed liquid, when the bacterial content reaches 10 7-8 At about CFU / ml, inoculate the first-level seed liquid at 5% of the inoculum amount into the second-level seed ...

Embodiment 3

[0045] Traditional Chinese medicine: 3 parts of dandelion, 3 parts of licorice, 1 part of Sophora flavescens, 2 parts of three needles, 1 part of Scutellaria baicalensis Georgi, 1 part of Artemisia annua, 1 part of wild chrysanthemum, 2 parts of Chinese neem, 1 part of rhubarb

[0046] Preparation:

[0047] (1) Inoculate Bacillus licheniformis and Bacillus Laterosporus into common nutrient broth, culture at 37℃, 180rpm shaker to produce first-grade seed liquid, when the bacterial content reaches 10 7-8 When CFU / ml is about, the first-level seed liquid is inoculated into the second-level seed fermentor with an inoculum amount of 3%, and the culture temperature is 32℃ and the rotation speed is 180rpm. When the spore content reaches 10 9 When CFU / ml is about, stop fermentation and reserve;

[0048] (2) Inoculate Candida utilis into the wort medium, culture at 28°C and 180rpm in a shaker to produce first-grade seed liquid, when the bacterial content reaches 10 7-8 When CFU / ml is about, th...

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Abstract

The invention discloses a disease-resistant growth promoter for aquatic animals and a preparation method thereof. The disease-resistant growth promoter is characterized in that a novel environmentally-friendly feed additive is prepared by combining the traditional Chinese medicine with a modern microbial transformation technology. The preparation method comprises the following steps: mixing dandelion, licorice root, lightyellow sophora root, barberry root and the like after crushing and screening; adding bran, soybean meal, corn flour and water as a fermentation culture medium, regulating thepH value, sterilizing, cooling, adding a strain, and fermenting; and drying after the fermentation is finished. The disease-resistant growth promoter for the aquatic animals is formed by virtue of beneficial combination of the traditional Chinese medicine and probiotics, can be used for replacing antibiotics and has the effects of resisting bacteria, preventing verminosis, resisting virus, improving the immunity, promoting the growth of the aquatic animals and the like; no toxicity, no side effects, no pollution, no residue and no drug resistance are produced during the long-term use of the disease-resistant growth promoter; and the effects are stable and reliable, and the requirements of people on safe production of aquatic products can be met. The disease-resistant growth promoter for the aquatic animals can also be applied as a veterinary drug by improving the using dose.

Description

Technical field [0001] The invention belongs to the field of feed additives, and relates to a disease-resistant growth-promoting agent for aquatic animals and a preparation method thereof. Background technique [0002] In recent years, with the improvement of people's living standards, the demand for aquatic products is also expanding, which to a large extent drives the rapid development of the aquaculture industry, and the scale of intensive industrial aquaculture is increasing. At the same time, diseases of aquatic animals occur frequently, and there are more than 100 kinds of fish diseases alone, of which more than 10 are serious. For example, viral outbreaks of shrimp in the early 1990s, and current outbreaks of scallops, river crabs, and soft-shelled turtles have brought huge losses to the aquaculture industry. Antibiotic treatment is currently the most important method of disease prevention and treatment. However, due to the widespread or improper use of antibiotics in pro...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/14A23K1/18A23K10/12A23K10/30A23K50/80
CPCY02A40/818
Inventor 肖传明刘春辉黄远荣
Owner BEIJING KEEPYOUNG TECH
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