Transfer factor solution applied in livestock and poultry, its preparation method and application thereof

A transfer factor and solution technology, applied in the field of bioengineering, can solve the problems of cumbersome procedures, inconvenient use and transportation, etc., and achieve the effects of releasing immunosuppression, making up for immune blanks, and improving extraction rate.

Inactive Publication Date: 2006-11-22
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing transfer factor technology is usually to remove the fat and large blood vessels in the spleen of animals and then extract them. certain inconvenience

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0011] The preparation method of the transfer factor solution applied to livestock and poultry of the present invention is to wash 500 g of frozen chicken spleen with distilled water and cut it into small pieces, add 1000 ml of pyrogen-free distilled water, break the cells with a tissue masher, and mix them evenly Made into a homogenate. Place at -30°C and freeze-thaw repeatedly 8 times, and the melting temperature should not exceed 37°C. The frozen-thawed homogenate was centrifuged at 4°C to remove the precipitate, and the supernatant was reserved for later use. The supernatant was filtered with a 0.45 μm microporous membrane, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5000 Daltons to obtain the stock solution. The original solution was prepared after removing the virus according to the national drug management method. During preparation, 2 ml of nicotinamide with a concentration range of 1.25%-2.5% is added as a stabilizer, an...

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Abstract

Disclosed is a transfer factor solution for animals, wherein its preparing process consists of washing freeze chicken spleens and chopping, charging distilled water and comminuting cells to homogenate, freezing eight times at minus 30 deg C, centrifuging the homogenated liquid, separating deposition and filtering supernatant fluid with microporous filtering film, carrying out hyperfiltration with ultrafiltration membrane to obtain stock solution, removing viruses, charging niacinamide as stabilizer, filtering and degerming through microporous filtering film, charging glycerine and split charging directly.

Description

technical field [0001] The invention belongs to a transfer factor used to improve the immunity of livestock and poultry in the technical field of bioengineering, and particularly relates to a transfer factor solution applied to livestock and poultry, a preparation method thereof and an application to livestock and poultry. Background technique [0002] With the continuous improvement of the scale and intensification of modern farming, viral diseases have become a major problem for farmers. The existing common solution is immunization. However, due to various reasons, sometimes it is inevitable that immunization fails and atypical infectious diseases break out, which brings certain difficulties to diagnosis and treatment. After immune failure, antibiotic treatment is often relied on, resulting in the increase of drug-resistant strains and the generation of cross-infection. As a cellular immune response enhancer, transfer factor can play an important role in the prevention an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/02A61P37/04
Inventor 李旭东鲍恩东苏建东
Owner TIANJIN RINGPU BIO TECH
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