Adsorption separation coupling fermentation process with high nisin yield,

A nisin, adsorption and separation technology, applied in the field of microorganisms, to achieve the effects of reducing production costs, good decolorization, and easy process control

Active Publication Date: 2014-12-17
西藏天虹科技股份有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the production of nisin is mainly by microbial fermentation. However, in the process of microbial fermentation to produce nisin, nisin has a significant inhibitory effect on cell growth, especially product synthesis, thereby inhibiting the production of nisin. In addition, the level of nisin-producing strains used for fermentation also directly restricts the yield of nisin, so the inhibition of nisin on cell growth and product synthesis is removed, and high-yield and excellent strains are used , will greatly increase the fermentation level of nisin, reduce production costs, and improve the industrial production efficiency of nisin

Method used

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  • Adsorption separation coupling fermentation process with high nisin yield,

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Effect test

Embodiment 1

[0035] Pick a ring of bacteria from the plate medium and inoculate it in the liquid seed culture medium, activate it at 30℃ for 8 hours, then insert it into another liquid seed culture medium at 1% of the inoculum, and cultivate it at 30℃ for 8 hours as the fermentation medium. Seed culture solution.

[0036] Fermentation medium: sucrose 2.9%, peptone 1.0%, yeast extract 0.4%, Na 2 HPO 4 1.8%, MgSO 4 0.02%, pH 7.5. The seed culture solution was inoculated into the fermentation medium, the inoculum amount was 5%, the culture temperature was 30°C, the pH value was 6.1, the rotation speed was 200 rpm, and the fermentation time was 5.5 hours by adding 2% D113 resin. The fermentation time was 24 hours. The fermentation concentration of nisin is 11325U / mL.

Embodiment 2

[0038] Pick a ring of bacteria from the plate medium and inoculate it in the liquid seed culture medium, activate it at 30℃ for 8 hours, then insert it into another liquid seed culture medium at 1% of the inoculum, and cultivate it at 30℃ for 8 hours as the fermentation medium. Seed culture solution.

[0039] Fermentation medium: sucrose 2.5%, peptone 0.8%, yeast extract 0.4%, Na 2 HPO 4 1.8%, MgSO 4 0.04%, pH 7.5. The seed culture solution was inoculated into the fermentation medium, the inoculum amount was 6%, the culture temperature was 30°C, the pH value was 6.2, the rotation speed was 300 rpm, and the fermentation time was 5.5 hours by adding 3% D113 resin, and the fermentation time was 24 hours. The fermentation concentration of nisin is 12085U / mL.

Embodiment 3

[0041] Pick a loop from the plate medium and connect it to the liquid seed culture medium, activate it at 30°C for 8 hours, then insert it into another liquid seed culture medium with 1% inoculum, and cultivate it at 30°C for 8 hours as the fermented seed culture liquid.

[0042] Fermentation medium: sucrose 3.0%, peptone 1.2%, yeast extract 0.5%, Na 2 HPO 4 2.0%, MgSO 4 0.03%, pH 7.5. The seed culture solution was inoculated into the fermentation medium, the inoculum amount was 5%, the culture temperature was 30°C, the pH value was 6.3, the rotation speed was 200 rpm, and the fermentation time was 5.5 hours with 5% NKA-II resin added. The fermentation time was 24 hours. At the end, the fermentation concentration of nisin was 11450 U / mL.

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Abstract

The invention discloses an adsorption separation coupling fermentation process with high nisin yield. A certain amount of resin is added in the logarithmic growth phase to adsorb the nisinin fermentation on the resin, thus on the one hand reducing concentration of nisin in the fermentation liquid to relieve the inhibitory effect on cell growth and product synthesis and on the other hand effectively improving the fermentation concentration of nisin. The nisin concentration in the invention reaches above 10000U / mL, which is significantly higher than the concentration of nisin in a general fermentation method. In addition, Lactococcus lactis CQ0422 with the preservation number of CGMCC NO.8090 is used as a fermentation strain, which has the yield of synthesized nisin at least 4.5 times of that of the general Lactococcus lactis, so as to play an important role in improving the nisin yield. The invention has the advantages of simple process, low production cost, concentration and high yield of nisin, etc.

Description

Technical field [0001] The invention relates to the technical field of microorganisms, in particular to a high-yield nisin adsorption separation coupled fermentation process. Background technique [0002] Nisin is both Nisin, also known as nisin or nisin. It is mainly a small molecule antimicrobial peptide produced by the metabolism of certain strains of nisin. It affects many including Staphylococcus aureus, hemolytic cocci, and botulinum. A variety of Gram-positive food spoilage bacteria and pathogenic bacteria, including Clostridium, Bacillus stearothermophilus and Listeria, have strong inhibitory effects. At present, the only microbial preservatives approved by my country's Food Additives Standardization Committee are Nisin and Natamycin. In 1990, my country listed it as the national standard GB2760-86, which can be used in the production of canned food, vegetable protein food, dairy products and meat products. Nisin is stable to heat, and is quickly digested into amino aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C07K14/315C07K1/14C12R1/46
Inventor 张春颖杨旭锦
Owner 西藏天虹科技股份有限责任公司
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