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Method for preparing natural medicines by coupling glycosidase catalysis and salting-out extraction

A technology of natural medicine and glycosidase, which is applied in the field of bioengineering, can solve problems such as no literature reports, achieve the effects of reducing production costs, simplifying production processes, and improving production efficiency

Inactive Publication Date: 2011-06-15
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Coupling extraction with fermentation or enzyme-catalyzed reaction to achieve separation while reacting is a research hotspot in recent years, but there is no literature report on the hydrolysis reaction catalyzed by glycosidase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Cellulase-catalyzed conversion of resveratrol glycosides coupled with aqueous two-phase extraction

[0021] Dissolve 5g of ammonium sulfate in 10.8g of water to form ammonium sulfate solution. The above inorganic salt solution was mixed with 4.2 g of ethanol dissolved in resveratrol glycosides, stirred evenly, and allowed to stand for 10 minutes to separate phases. Slowly add cellulase to the ethanol phase, and stir the upper phase to react for 10 h. The reaction temperature was 25° C., and the stirring speed was 70 rpm. The results of the analysis showed that 100% of the resveratrol glycosides were distributed in the upper phase and completely converted to resveratrol within 10 hours. Compared with the enzyme-catalyzed reaction in the water phase, it takes 12 hours for the conversion rate of resveratrol to reach 100% under the same conditions, and the existence of the two-water phase significantly shortens the reaction time.

Embodiment 2

[0022] Example 2: Coupling of helicase-catalyzed transformation of Dioscorea scutellaria and aqueous two-phase extraction

[0023] Dissolving crude saponin in 1,4-butanediol to prepare 1,4-butanediol saponin liquid. Get 3.36g of ammonium sulfate and 11.04g of water to make ammonium sulfate solution. The above inorganic salt solution was mixed with 3.6g of 1,4-butanediol saponin solution, stirred and allowed to stand for 10 minutes for phase separation. Analysis of the distribution of various saponins showed that 20% of diosgenin was distributed in the upper phase (1,4-butanediol phase), and 80% of diosgenin was distributed in the lower phase (ammonium sulfate phase), while scutelin, The distribution of diosgenin, glucotriglycoside, glucobioside and trillinoside in the upper and lower phases is equal to half. The helicase solution was slowly added to the upper phase, the reaction temperature was 50° C., the stirring speed was 70 rpm, and the reaction was carried out for 48 ho...

Embodiment 3

[0024] Example 3: Coupling of helicase-catalyzed transformation of diosgenin from scutellum leaf and three-liquid phase extraction

[0025] Dissolving crude saponin in 1,4-butanediol to prepare 1,4-butanediol saponin liquid. Get 3.36g of ammonium sulfate and 11.04g of water to make ammonium sulfate solution. The above inorganic salt solution was mixed with 3.6g of 1,4-butanediol saponin solution and 2.0g of petroleum ether, stirred and left standing for 10min to form three liquid phases. Analysis of the distribution of various saponins shows that 95% of diosgenin is distributed in the upper phase (petroleum ether phase), 5% of diosgenin is distributed in the middle phase (1,4-butanediol phase), and 80% of diosgenin is distributed in the upper phase (petroleum ether phase). Glycosides, glucobiosides, and trillidin were distributed in the middle phase (1,4-butanediol phase), and the remaining 20% ​​were distributed in the lower phase (ammonium sulfate phase). The helicase solu...

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PUM

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Abstract

The invention discloses a method for preparing natural medicines by coupling glycosidase catalysis and salting-out extraction, and belongs to the technical field of bioengineering. The method is characterized in that enzymatic hydrolysis reaction is coupled with salting-out extraction of a product. Glycosidase and the natural product are added into a salting-out extraction system, and the glycosidase is utilized to convert the natural product containing glycosyl into an active ingredient of which the glycosyl is removed; meanwhile, the product and a substrate are separated through the extracting capacity of the system. The invention has the advantages of developing a novel reaction and separation coupled method easy for industrial production, and improving production efficiency.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for preparing medicine or a medicine intermediate by coupling salting-out extraction and glycosidase hydrolysis reaction. Background technique [0002] Natural products have gradually become an important source of medicine, food and feed, and health food and medicine based on natural products currently have a considerable market. Glycoside natural products occupy an important position in many natural products, and a series of glycosides with biological activity and medicinal value have been put into use one after another, such as gastroside, arbutin, digitalisin, platycodon saponin, ginsenoside, etc. The activity of glycosides is often related to the type and quantity of sugar groups. Changes in sugar groups can cause changes in activity. Many aglycones have stronger biological activities than glycosides. Therefore, research on glycoside hydrolysis has attracted wid...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12P19/20C12P19/16
Inventor 修志龙魏夺董悦生韩松孙亚琴
Owner DALIAN UNIV OF TECH
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