Apparatus and method for butanol production through dual bacteria immobilization anaerobic fermentation

A technology for anaerobic fermentation and butanol production, which is applied in the field of bioengineering, can solve the problems of low butanol yield, yield and ABE yield and yield, so as to shorten the fermentation cycle, improve the utilization rate, and improve the output per unit time. Effect

Active Publication Date: 2013-03-06
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method butanol yield, productive rate and A

Method used

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  • Apparatus and method for butanol production through dual bacteria immobilization anaerobic fermentation
  • Apparatus and method for butanol production through dual bacteria immobilization anaerobic fermentation
  • Apparatus and method for butanol production through dual bacteria immobilization anaerobic fermentation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1

[0032] Bacterial species: Clostridium tyrobutyricum ATCC 25755 (Clostridium tyrobutyricum) is the butyric acid-producing bacteria, and Clostridium beijerinckii ATCC 55025 (Clostridium beijerinckii) is the butanol-producing bacteria.

[0033] Seed Medium:

[0034] Seed medium for Clostridium beijerinckii Ⅰ (CM 149) ( / L): Yeast extract 3g, beef extract 10g, peptone 10g, glucose 5g, soluble starch 1g, NaCl 5g, sodium acetate 3g, cysteine ​​hydrochloride 0.5 g, carbon source and nitrogen source are sterilized separately, pH 6.8±0.2.

[0035] Clostridium tyrobutyricum seed medium II (RCM) ( / L): mineral 1 solution 40mL; mineral 2 solution 40mL, trace metal element solution 10mL, vitamin solution 10mL, 0.005%NiCl 6H 2 O10mL, 0.2%FeSO 4 ·7H 2 O 2mL, glucose 30g, peptone 5g, yeast extract 5g, NaCl 6g, cysteine-HCl 0.3g, 0.1% resazurin 0.5mL, boil in a boiling water bath to remove oxygen, carbon source and nitrogen source are sterilized separately , pH 6.0.

[0...

Example Embodiment

[0057] Example 2

[0058] Bacterial species: Clostridium tyrobutyricum ATCC 25755 (Clostridium tyrobutyricum) is the butyric acid-producing bacterium, and Clostridium beijerinckiiATCC 55025 (Clostridium beijerinckii) is the butyric acid-producing bacterium.

[0059] Seed medium:

[0060] Seed medium for Clostridium beijerinckii Ⅰ (CM 149) ( / L): Yeast extract 3g, beef extract 10g, peptone 10g, glucose 5g, soluble starch 1g, NaCl 5g, sodium acetate 3g, cysteine ​​hydrochloride 0.5 g, carbon source and nitrogen source are sterilized separately, pH 6.8±0.2.

[0061] Clostridium tyrobutyricum seed medium II (RCM) ( / L): mineral 1 solution 40mL; mineral 2 solution 40mL, trace metal element solution 10mL, vitamin solution 10mL, 0.005%NiCl 6H 2 O10mL, 0.2%FeSO 4 ·7H 2 O 2mL, glucose 30g, peptone 5g, yeast extract 5g, NaCl 6g, cysteine-HCl 0.3g, 0.1% resazurin 0.5mL, boil in a boiling water bath to remove oxygen, carbon source and nitrogen source are sterilized separately , pH 6.0....

Example Embodiment

[0078] Example 3

[0079] Bacterial species: Clostridium tyrobutyricum ATCC 25755 (Clostridium tyrobutyricum) is the butyric acid-producing bacterium, and Clostridium tyrobutyricum ATCC 55025 (Clostridium beijerinckii) is the butanol-producing bacterium.

[0080] Seed medium:

[0081] Seed medium for Clostridium beijerinckii Ⅰ (CM 149) ( / L): Yeast extract 3g, beef extract 10g, peptone 10g, glucose 5g, soluble starch 1g, NaCl 5g, sodium acetate 3g, cysteine ​​hydrochloride 0.5 g, carbon source and nitrogen source are sterilized separately, pH 6.8±0.2.

[0082] Clostridium tyrobutyricum seed medium II (RCM) ( / L): mineral 1 solution 40mL; mineral 2 solution 40mL, trace metal element solution 10mL, vitamin solution 10mL, 0.005%NiCl 6H 2 O10mL, 0.2%FeSO 4 ·7H 2 O 2mL, glucose 30g, peptone 5g, yeast extract 5g, NaCl 6g, cysteine-HCl 0.3g, 0.1% resazurin 0.5mL, boil in a boiling water bath to remove oxygen, carbon source and nitrogen source are sterilized separately , pH 6.0.

...

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Abstract

The present invention discloses an apparatus and a method for butanol production through dual bacteria immobilization anaerobic fermentation, wherein the apparatus comprises a fermentation culture medium storage tank, a butyric acid production immobilization reactor A, a butanol production immobilization reactor C, and a product collection tank, wherein the devices are sequentially connected in series through pipelines. According to the present invention, butyric acid production anaerobic bacteria and butanol production anaerobic bacteria are respectively immobilized in the butyric acid production immobilization reactor A and the butanol production immobilization reactor C to carry out dual bacteria immobilization, and a fermentation culture medium is controlled to flow from the fermentation culture medium storage tank, sequentially flows through the butyric acid production immobilization reactor A and the butanol production immobilization reactor C to carry out continuous anaerobic fermentation, and finally flows into the product collection tank, wherein the butyric acid production anaerobic bacteria adopts a substrate to produce butyric acid, and the butanol production anaerobic bacteria adopts the butyric acid as a substrate to produce butanol. With the method, a fermentation period can be substantially shortened, and substrate conversion rate and yield of the butanol can be increased.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a production device and method of butanol, in particular to a device and a method for producing butanol by immobilized double bacteria anaerobic fermentation. Background technique [0002] Butanol is a four-carbon saturated alcohol, also known as n-butanol, with the molecular formula C 4 h 9 OH, colorless, with a special smell, completely miscible with organic solvents, and partially miscible with water. [0003] N-butanol is a good solvent for the production of antibiotics, vitamins and hormones. It is also used in the manufacture of n-butyl plasticizers of aliphatic dibasic acids, phthalic acid and phosphoric acid. In addition, it is also used in the manufacture of butyl acetate, acrylic acid Butyl ester, ethylene glycol butyl ether, as an intermediate in organic synthesis and in the manufacture of surfactants, etc. Butanol is also an excellent diluent in brake fluid formulations...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12P7/16C12R1/145
Inventor 王菊芳李林李爽于平儒
Owner SOUTH CHINA UNIV OF TECH
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