Method for enhancing mitsuba seed germination rate
A kind of clover, germination rate technology, applied in the field of agriculture and forestry
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Embodiment 1
[0021] Prepare saline with a mass concentration of 15%, benomyl at 500 times liquid, gibberellin solution at 250 mg / L, and naphthaleneacetic acid solution at 45 mg / L. Soak the celery seeds in 15% salt water for 10 minutes, and remove the floating sundries and deflated seeds, then wash the seeds with clean water, then put the washed celery seeds in 500 times liquid benomyl Soak in (benomyl weight unit 1: water weight unit 500) for 5 hours, remove and drain excess water, and then put the drained celery seeds in a concentration of 250mg / L at 25°C±2°C Soak in the gibberellin solution for 12±0.5 hours, wrap the celery seeds soaked in the gibberellin with gauze to prevent the seeds from scattering, and soak in warm water whose water temperature gradually drops from 55°C to 35°C for 6 hours. hours (at this time, the cooling rate of warm water is 3-3.6°C / hour). After soaking at variable temperature, spread the seeds evenly on the surface of the sponge block (germination requires ligh...
Embodiment 2
[0023] The difference between this example and Example 1 is that saline with a mass concentration of 18%, benomyl with 1000 times liquid, 300 mg / L gibberellin solution, and 20 mg / L indole acetic acid solution were prepared. After the clover seeds were soaked in saline with a mass concentration of 18% for 8 minutes, the clover seeds were soaked in 1000 times liquid benomyl (benomyl weight unit 1: water weight unit 1000) for 10 hours, and then Soak in 300mg / L gibberellin solution at 25±2°C for 11.5 hours, place the seeds in a breeding tray and add 20mg / L indole acetic acid to the breeding tray, accelerate germination under variable temperature conditions, and then observe And record the germination rate, germination potential and germination index of clover seeds in 7 days, and the fresh weight per plant, bud length and lateral root number of seedlings, and record it as treatment 2, the results are shown in Table 1.
Embodiment 3
[0025] The difference between this example and Example 1 is that saline with a mass concentration of 16%, benomyl with 700 times liquid, and 200 mg / L gibberellin solution were prepared. After soaking the clover seeds in saline with a mass concentration of 16% for 12 minutes, soak the clover seeds in 700 times liquid benomyl for 7 hours, and then soak them in 25±2°C at a concentration of 200mg / Soak in the gibberellin solution of L for 12.5 hours, seed is placed in the breeding dish and adds the gibberellin of 200mg / L to the breeding dish, vernalizes under variable temperature conditions, then observes and records the germination rate of celery seed in 7 days, The germination potential and germination index, as well as the fresh weight per plant, bud length and lateral root number of the seedlings were recorded as treatment 3, and the results are shown in Table 1.
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